bmo-miR-0001 and bmo-miR-0015 down-regulate expression of <Emphasis Type="Italic">Bombyx mori</Emphasis> fibroin light chain gene in vitro     

Chen Chen  Yang-yang Fan  Xin Wang  Fei Song  Tao Jiang  Ping Qian  Shun-ming Tang  Xing-jia Shen 《Journal of Zhejiang University. Science. B》2016,17(2):127-135

Based on bioinformatic analysis, we selected two novel microRNAs (miRNAs), bmo-miR-0001 and bmomiR-0015, from high-throughput sequencing of the Bombyx mori larval posterior silk gland (PSG). Firstly, we examined the expression of bmo-miR-0001 and bmo-miR-0015 in 12 different tissues of the 5th instar Day-3 larvae of the silkworm. The results showed that the expression levels of both bmo-miR-0001 and bmo-miR-0015 were obviously higher in the PSG than in other tissues, implying there is a spatio-temporal condition for bmo-miR-0001 and bmo-miR-0015 to regulate the expression of BmFib-L. To test this hypothesis, we constructed pri-bmo-miR-0001 expressing the plasmid pcDNA3.0 [ie1-egfp-pri-bmo-miR-0001-SV40] and pri-bmo-miR-0015 expressing the plasmid pcDNA3.0 [ie1-egfp-pribmo-miR-0015-SV40]. Finally, the BmN cells were harvested and luciferase activity was detected. The results showed that luciferase activity was reduced significantly (P<0.05) in BmN cells co-transfected by pcDNA3.0 [ie1-egfp-pri-bmomiR-0001-SV40] or pcDNA3.0 [ie1-egfp-pri-bmo-miR-0015-SV40] with pGL3.0 [A3-luc-Fib-L-3′UTR-SV40], suggesting that both bmo-miR-0001 and bmo-miR-0015 can down-regulate the expression of BmFib-L in vitro.  相似文献   

试论当代高校教师的核心素质   总被引：1，自引：0，他引：1  

沈春敏  沈丽巍 《长春工业大学学报(高教研究版)》2008,29(3)

知识经济时代,经济全球化的含义已经远远超出经济范畴,国际间的经济竞争实质是高素质人才的较量与竞争。人才的培养靠教育。提高高校教育质量,教师是关键。高校教师必须加强自身核心素质的提高,以适应培养知识、能力等全面发展的复合型人才的需要。  相似文献   

启发式教学方法在电工电子实验教学中的运用     

申文达  董云凤  李可 《实验技术与管理》2012,(4):265-266

在电工电子实验教学中,教师既要注重培养学生掌握基本知识、方法和技能,又要培养学生的主动学习意识、独立思考能力和实践创新能力。采用启发式教学方法是我们教学的指导思想。该文通过一次实验课的教学实践,来说明启发式教学方法在电工电子实验教学中是如何运用的。  相似文献   

应用型人才培养模式下电工学课程改革与实践     

高有华  申永山  龚淑秋  韩颖  徐卜一 《实验技术与管理》2012,(4):272-273,277

针对应用型本科教育模式对学科基础课的要求,对电工学课程的教学方法、教学手段和实验改革等进行了深入的探索与实践,提出通过明确教学目标,改革教学方法、教学手段和实验教学内容,构建以能力培养为重心的教学体系和实践学习新模式,有效地提高教育、教学效果。  相似文献   

Effective practices for creating transformative informal science education programs grounded in Native ways of knowing     

Elizabeth Mack  Helen Augare  Linda Different Cloud-Jones  Dominique Davíd  Helene Quiver Gaddie  Rose E. Honey  Angayuqaq O. Kawagley  Melissa Little Plume-Weatherwax  Lisa Lone Fight  Gene Meier  Tachini Pete  James Rattling Leaf  Elvin Returns From Scout  Bonnie Sachatello-Sawyer  Hi’ilani Shibata  Shelly Valdez  Rachel Wippert 《Cultural Studies of Science Education》2012,7(1):49-70

There are a growing number of informal science education (ISE) programs in Native communities that engage youth in science education and that are grounded in Native ways of knowing. There is also a growing body of research focusing on the relationship between culture, traditional knowledge, and science education. However, there is little research documenting how these programs are being developed and the ways in which culture and Western science are incorporated into the activities. This study outlines effective practices for using Native ways of knowing to strengthen ISE programs. These effective practices may also be used to promote change in formal education. The authors combine an overview of current research in informal science education with personal interviews with educators engaged in ISE programs offered to youth both on and off tribal reservations as well as experts in Indigenous education. Participating individuals and programs included Native communities across the United States, including Alaska and Hawai??i. Keeping in mind that each community is unique, ISE programs that are grounded in Native ways of knowing will benefit by utilizing the effective practices outlined here as a guide for starting or strengthening existing ISE programs relevant to the needs of their communities.  相似文献   

数字图书馆推广工程标准规范体系建设规划与实践     

赵悦  申晓娟胡洁李丹 《国家图书馆学刊》2012,21(5):46-53,59

标准规范是现代图书馆发展的一个重要基础,是促进数字图书馆推广工程快速、经济和可持续发展的重要条件。推广工程自建设伊始就非常重视标准规范体系的规划与建设,并制定了数字图书馆推广工程标准规范体系建设原则及框架。今后,还将针对重点领域加快标准规范的制修订,完善标准规范开放建设机制,推进已有标准规范成果的推广与应用,进一步完善工程标准规范体系。图1。参考文献9。  相似文献   

基于QoS的认知无线电网络MAC协议(英文)   总被引：1，自引：0，他引：1  

胡静  沈连丰  宋铁成 《东南大学学报》2012,(4):375-379

为提高认知环境中次用户的吞吐量性能,提出了一种基于QoS的MAC协议. 认知节点将信道状态映射为向量,收端与发端通过"与"操作得到最终的信道映射,并获得备选信道集合. 上层应用程序传来的数据根据QoS要求被划分为2个优先级,每种级别的数据对应不同的竞争窗口,从而保证了实时数据具有较高的优先级. 使用二维离散时间马尔科夫链分析了系统的性能并推导出系统吞吐量的数学表达式. 仿真结果证实,与IEEE 802.11 DCF相比,所提协议有助于提高系统吞吐量.  相似文献   

迎接挑战——讲活电子技术课     

龚淑秋  李静  高有华  申永山 《实验技术与管理》2012,(4):234-236

根据电子技术课程的特点,将电子技术课程内容模块化,改革、继承、活化传统的教学模式,在教学方法上倡导巧妙的"四两拨千斤"。系统培养学生善于学习的能力,讲活电子技术课,以迎接课程内容多、学时少的挑战。  相似文献   

Investigating the Effect of Item Position in Computer‐Based Tests     

Feiming Li  Allan Cohen  Linjun Shen 《Journal of Educational Measurement》2012,49(4):362-379

Computer‐based tests (CBTs) often use random ordering of items in order to minimize item exposure and reduce the potential for answer copying. Little research has been done, however, to examine item position effects for these tests. In this study, different versions of a Rasch model and different response time models were examined and applied to data from a CBT administration of a medical licensure examination. The models specifically were used to investigate whether item position affected item difficulty and item intensity estimates. Results indicated that the position effect was negligible.  相似文献   

幼儿园教育科研中的普遍问题与应对     

曾莉  彭丰  申晓燕 《学前教育研究》2012,(4):64-66

当前幼儿园教育科研中普遍存在选题盲目不清、教师科研素质较低、日常教学与科研难以协调、缺乏制度保障等问题。为推进幼儿园教科研工作,幼儿园在选题上应从微观入手,切合本园实际;应建立教研制度,形成科研常态;应构建二元一体模式,回归日常教育生活研究,从而为幼儿园发展提供动力与活力。  相似文献