Evaluation of Quality of DNA Extracted from Buccal Swabs for Microarray Based Genotyping     

Alex Livy  Sayhean Lye  Chahil K. Jagdish  Nurul Hanis  Velapasamy Sharmila  Lian Wee Ler  Bagali Pramod 《Indian journal of clinical biochemistry : IJCB》2012,27(1):28-33

Buccal cell usage has been shown by many to be a cost effective and safe method to isolate DNA for various biological experiments especially large epidemiological studies (Garcia-Closas et al. Cancer Epidemiol Biomarkers Prev 10:687–696, 2001). Non-invasive DNA collection methods are preferred over phlebotomy in order to increase study participation and compliance in research centers and for sick patients in hospital settings. There have been conflicting reports about the methodology and results obtained from using buccal DNA. It is not very clear if phlebotomy can be confidently replaced by buccal cell DNA. It is often left for the user to take an intelligent decision. To address this issue, we compared the performance of buccal and blood DNA from same subjects in a genotyping experiment and this paper reports the results. Cotton swab derived buccal cells were scraped from the inner side of cheeks from 16 subjects, and blood was also drawn from the same 16 subjects participating in a genotypic association study of a lipid disease. The DNA quality was assessed by resolving on agarose gels, checking purity (A260/A280) and finally by microarray hybridization. This study showed that DNA degradation affects the total yield and performance of the buccal DNA when compared to the blood DNA in microarray based genotyping. Genotyping results can be seriously compromised if care is not taken to check the quality and yields of such specimens.  相似文献   

企业文化中心法则构建机制研究     

马晓苗  石冠峰 《科技管理研究》2012,32(7):227-230

结合生物中心法则,提出了企业文化中心法则。企业文化的三个层次——核心价值观、制度/技术文化以及行为/形象文化分别对应于生物大分子DNA、RNA和蛋白质。企业文化三层次间也存在着信息复制、转录和转译的过程。企业文化中心法则的构建,为我们探讨企业文化层次要素间的信息传递、企业文化的运行演化规律以及构建良好的企业文化提供了新的视角和思路。  相似文献   

Questioning Race: Ancestry DNA and Dialog on Race     

《Communication monographs》2012,79(2):271-290

Human genetics and racial identity converge pointedly in the family narrative. Until recently, genetics, racial identity, and family narrative were all rather malleable concepts in the public arena. All were presented in anecdotal form for the most part, and were often based on certain social conventions. The interjection of popularly available ancestry DNA data adds an additional piece of information to the discussion of genetics, race, and narrative. Using the framework of both narrative theory and theory of social construction, this work uses quantitative and qualitative data to explore how individuals react to ancestry DNA findings and to consider if and how this information will change narratives, behaviors, and perspectives. We also explore whether one's racial identification makes a difference in initial accuracy and if there is a difference among racial groupings in terms of change in census identification based on knowledge of their DNA profile.  相似文献   

家蚕微孢子DNA的PCR检测灵敏度研究     

潘中华  贡成良  郑小坚  薛仁宇  曹广力  沈卫德 《常熟理工学院学报》2007,21(4):51-54

对纯化的家蚕微孢子的DNA抽提及PCR检测灵敏度进行了研究。结果显示:以0.1mol/L K2CO3 0.1mol/L KHCO3作为诱导剂诱导人工纯化的微孢子发芽是最佳选择,配合proteaseK法,能较好地抽提微孢子的DNA;PCR检测灵敏度研究发现,最低检测微孢子核酸浓度是3.25×10-2pg(25μl反应体系),溶液中只要有4粒微孢子即可检出。  相似文献   

乳癌细胞核DNA含量与预后     

雷杰  张振忠  李德仪 《河北北方学院学报(医学版)》1999,(3)

本文应用流式细胞术(Flow Cytometry,FCM)检测了102例石蜡包埋的乳癌组织的细胞核DNA含量,并探讨了DNA指数(DNA Index.DI)和S期细胞比例(S-phase Fraction,SPF)与临床预后因素及病人术后生存状况的关系。结果显示:16例为DNA二倍体,8例近二倍体,78例(77％)非整倍体。非整倍体癌较二倍体/近二倍体癌的SPF高。DI值与组织学分级、淋巴结状况、原发肿瘤大小和临床分期密切相关,而与病人诊断时的年龄无关。淋巴结转移阳性组较阴性组、晚期肿瘤(Ⅲ、Ⅳ期)比早期肿瘤(Ⅰ、Ⅱ期)的DI值高(P<0.05),而非整倍体率仅有降低的趋势但无显著性差异,说明DI值作为一个预后参数比倍性分析统计效率高。SPF与病人的各预后因素及生存情况无明显关系。二倍体/近二倍体癌病人较非整倍体癌病人的生存率高、无瘤生存时间长,说明流式细胞术DNA含量测定确实可为乳癌病人的预后提供有价值的信息。  相似文献   

旱莲草对老年小鼠脾淋巴细胞的作用     

冯明功  才迎陈群 《大连大学学报》1999,20(4):74-77,80

方法：观察了老年小鼠服旱莲草水煎剂后,脾淋巴细胞IL2、MAF的分泌和DNA合成的变化;并观察了旱莲草体外对脾淋巴细胞DNA合成的影响．结果：①使老年小鼠脾淋巴细胞分泌IL2逐渐减少（P＜0.001）,并呈剂量依赖关系（r=-8988）;②体内外实验表明高浓度旱莲草水煎剂抑制脾淋巴细胞在ConA激活后的DNA合成,但无ConA时DNA合成不受影响;③老年小鼠脾淋巴细胞分泌MAF能力明显低于青年小鼠（P＜0.05）,灌服旱莲草24g/kg×7d使老年小鼠脾淋巴细胞分泌MAF能力明显增强（P＜0.05）．  相似文献   

手性氨基酸对DNA链式聚合反应的影响     

卜新雅  陈露  阚玉贺  雷华军  何裕建  封禄田  吴丽 《中国科学院大学学报》2021,38(3):306-313

构成天然蛋白质的氨基酸为L构型,D构型氨基酸虽然不是构成蛋白质的基本结构单元,但许多植物、微生物甚至在人体中都有D-氨基酸的存在.DNA聚合酶链式反应广泛发生在生物体内及PCR实验中,首次探究手性氨基酸及其与金属离子协同条件下对Taq-DNA聚合酶催化下的DNA链式聚合反应的影响情况,为进一步研究手性氨基酸及金属离子对...  相似文献   

HBV核酸光敏生物素探针的制备     

李弘华 《孝感职业技术学院学报》2003,6(3):71-73

利用设计合成的特异性引物，通过PCR扩增获得乙肝病毒的S区621个bpDNA片段．该扩增产物经EcoRI与BamHI双酶切后直接克隆进入PSK载体进行表达，再通过扩增重组菌株，以获得大量的S区DNA片段，对它们进行光敏生物素标记，制成探针，对乙肝病毒进行检测灵敏度高，特异性强，其灵敏度可达到4pg水平。  相似文献   

酶定向进化的研究进展     

雷启义 《凯里学院学报》2005,23(3):25-27

酶的体外定向进化是改造生物催化剂的一种有效的新策略,主要是模拟自然进化进程,通过易错PCR等技术对编码酶的基因进行随机突变,再由DNA改组、交错延伸过程、随机引导重组、递增截短技术等进行突变基因的体外重组,最终经筛选获得所需的酶.本文综述了酶定向进化的研究进展情况.  相似文献   

计算机软件在PCR实验上的应用     

黄子锋  周厚高  王凤兰  黄玉源  王鸿昌 《实验室研究与探索》2004,23(10):45-48

对与PCR实验紧密相关的计算机软件——引物设计软件、凝胶图像处理软件、数据分析软件和文献查找软件的应用进行了概述。  相似文献