Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca<Superscript>2+</Superscript> transport proteins and the effect of taurine on myocardial protection in rabbits     

Huang?Xian-meiEmail author  Zhu?Wei-hua  Kang?Man-li 《浙江大学学报(A卷英文版)》2003,4(1):114-120

To investigate the effect of doxorubicin(DOX) on gene expression of the myocardial sarcoplasmic reticulum (SR) Ca²⁺ transport proteins and the mechanism of taurine(Tau) protecting cardiac muscle cells, 9 rabbits were injected with DOX, 8 rabbits with DOX and Tau, and 9 rabbits with normal saline. Cardiac function, concentration of calcium in cardiomycocytes (Myo [Ca²⁺] _i ), activity of SR Ca²⁺-ATPase (SERCA2a), level of SERCA2a mRNA and Ca²⁺ released channels (RYR2) mRNA were detected. The left ventricle tissues were observed by electron microscopy. The results showed that cardiac index, left ventricular systolic pressure, activity of SR Ca²⁺-ATPase and level of SERCA2a mRNA decreased, while Myo[Ca²⁺] _i increased in DOX-treated rabbits. DOX could not affect the level of RYR2 mRNA. Tau intervention could alleviate the increase of left ventricular diastolic pressure, Myo[Ca²⁺] _i and the decrease of SERCA2a mRNA induced by doxorubicin. The results suggested that downregulation of SERCA2a gene expression was an important mechanism of DOX-induced cardiomyopathy and that Tau could partially improve the heart function by reducing calcium overload and alleviating downregulation of SERCA2a mRNA.  相似文献   

BP神经网络优化算法研究     

杨丽芬  蔡之华 《教育技术导刊》2007,(5)

为解决BP神经网络收敛速度慢和易陷入局部极小值的缺点,利用遗传算法(GA)和基因表达式编程(GEP)的各自特点,基于BP算法提出了两种改进算法:其一是GA-BP算法,即利用GA优化BP神经网络的权值和阈值;其二是GEP-BP算法,即利用GEP对BP网络进行调整,包括网络结构、权值和阈值。用样本数据进行了测试并与基本BP算法进行了比较,结果表明两种改进算法具有很强的可行性和高效性。  相似文献   

Characterization of enhancer trap and gene trap harboring<Emphasis Type="Italic">Ac/Ds</Emphasis> transposon in transgenic rice     

Jin Wei-zheng  Wang Shao-min  Xu Min  Duan Rui-jun  Wu Ping 《浙江大学学报(A卷英文版)》2004,5(4):390-399

Insertion mutagenesis has become one of the most popular methods for gene functions analysis. Here we report a two-elementAc/Ds transposon system containing enhancer trap and gene trap for gene tagging in rice. The excision ofDs element was examined by PCR amplification. The excision frequency ofDs element varied from 0% to 40% among 20 F₂ populations derived from 11 differentDs parents. Southern blot analysis revealed that more than 70% of excisedDs elements reinserted into rice genome and above 70% of the reinsertedDs elements were located at different positions of the chromosome in rice. The result of histochemical GUS analysis indicated that 28% of enhancer trap and 22% of gene trap tagging plants displayed GUS activity in leaves, roots, flowers or seeds. The GUS positive lines will be useful for identifying gene function in rice. Project supported by the Hi-Tech Research and Development Program (863) of China (No. 2002AA2Z1003)  相似文献   

IGF-1基因多态性与骨骼肌性状的关系   总被引：1，自引：0，他引：1  

范刚  王志跃 《广州体育学院学报》2008,28(5)

运动员选材是竞技体育中取胜的关键之一.在后基因组时代,基因选材将成为运动选材的重要手段,而基因选材的前提是必须找到有效的基因标记.胰岛素样生长因子-1(IGF-1)是一种与肌肉、骨骼等组织的生长关系十分密切的小分子多肽,其基因上很可能存在运动能力分子标记.因此,以该基因为目标区域,进行了查找基因标记的尝试.用PCR-RFLP法检测了基因5'调控区单核苷酸多态(SNPs)情况,并用广义线性模型统计分析了多态性与肌肉性状的关系.结果显示:IGF-1基因5'调控区存在一个C-T转换突变,形成三种基因型,分别标记为:"-/-"、"-/ "、" / ".基因型与骨骼肌生长总量和肌纤维直径有着密切的关系,"-/-"基因型个体的胸部和腿部肌肉总量最高,与" / '基因型个体相比差异显著,P值分另q为0.029和0.038,而且"-/-"基因型个体的肌纤维平均直径最大,与" /-"基因型比差异显著(P＜0.05).  相似文献   

Conditional gene manipulation: Cre-ating a new biological era     

Jian Zhang  Jing Zhao  Wen-jie Jiang  Xi-wei Shan  Xiao-mei Yang  Jian-gang Gao 《Journal of Zhejiang University. Science. B》2012,13(7):511-524

To solve the problem of embryonic lethality in conventional gene knockouts,site-specific recombinase(SSR)systems(Cre-loxP,Flp-FRT,andΦC31)have been used for tissue-specific gene knockout.With the combination of an SSR system and inducible gene expression systems(tetracycline and tamoxifen),stage-specific knockout and transgenic expression can be achieved.The application of this"SSR+inducible"conditional tool to genomic manipulation can be extended in various ways.Alternatives to conditional gene targeting,such as conditional gene trapping,multipurpose conditional alleles,and conditional gene silencing,have been developed.SSR systems can also be used to construct precise disease models with point mutations and chromosomal abnormalities.With these exciting achievements,we are moving towards a new era in which the whole genome can be manipulated as we wish.  相似文献   

Antitumor effects of human interferon-alpha 2b secreted by recombinant bacillus Calmette-Guérin vaccine on bladder cancer cells     

Guo-qing Ding  Yan-lan Yu  Zhou-jun Shen  Xie-lai Zhou  Shan-wen Chen  Guo-dong Liao  Yue Zhang 《Journal of Zhejiang University. Science. B》2012,13(5):335-341

Objective:Our objective was to construct a recombinant bacillus Calmette-Guérin vaccine(rBCG) that secretes human interferon-alpha 2b(IFNα-2b) and to study its immunogenicity and in vitro antitumor activity against human bladder cancer cell lines T24 and T5637.Methods:The signal sequence BCG Ag85B and the gene IFNα-2b were amplified from the genome of BCG and human peripheral blood,respectively,by polymerase chain reaction(PCR).The two genes were cloned in Escherichia coli-BCG shuttle-vector pMV261 to obtain a new recombinant plasmid pMV261-Ag85B-IFNα-2b.BCG was transformed with the recombinant plasmid by electroporation and designated rBCG-IFNα-2b.Mononuclear cells were isolated from human peripheral blood(PBMCs) and stimulated with rBCG-IFNα-2b or wild type BCG for 3 d,and then cultured with human bladder cancer cell lines T24 and T5637.Their cytotoxicities were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay.Results:BCG was successfully transformed with the recombinant plasmid pMV261-Ag85B-IFNα-2b by electroporation and the recombinant BCG(rBCG-IFNα-2b) was capable of synthesizing and secreting cytokine IFNα-2b.PBMC proliferation was enhanced significantly by rBCG-IFNα-2b,and the cytotoxicity of PBMCs stimulated by rBCG-IFNα-2b to T24 and T5627 was significantly stronger in comparison to wild type BCG.Conclusions:A recombinant BCG,secreting human IFNα-2b(rBCG-IFNα-2b),was constructed successfully and was superior to control wild type BCG in inducing immune responses and enhancing cytotoxicity to human bladder cancer cell lines T24 and T5637.This suggests that rBCG-IFNα-2b could be a promising agent for bladder cancer patients in terms of possible reductions in both clinical dosage and side effects of BCG immunotherapy.  相似文献   

Receptor-mediated gene delivery using polyethylenimine （PEI） coupled with polypeptides targeting FGF receptors on cells surface     

Li D  Wang QQ  Tang GP  Huang HL  Shen FP  Li JZ  Yu H 《Journal of Zhejiang University. Science. B》2006,7(11):906-911

Objective： To construct a novel kind of nonviral gene delivery vector based on polyethylenimine （PEI） conjugated with polypeptides derived from ligand FGF with high transfection efficiency and according to tumor targeting ability. Methods： The synthetic polypeptides CR16 for binding FGF receptors was conjugated to PEI and the characters of the polypeptides including DNA condensing and particle size were determined. Enhanced efficiency and the targeting specificity of the synthesized vector were investigated in vitro and in vivo. Results： The polypeptides were successfully coupled to PEI. The new vectors PEI-CR16 could efficiently condense pDNA into particles with around 200 nm diameter. The PEI-CR16/pDNA polyplexes showed significantly greater transgene activity than PEI/pDNA in FGF receptors positive tumor cells in vitro and in vivo gene transfer, while no difference was observed in FGF receptors negative tumor cells. The enhanced transfection efficiency of PEI-CR 16 could be blocked by excess free polypeptides. Conclusion： The synthesized vector could improve the efficiency of gene transfer and targeting specificity in FGF receptors positive cells. The vector had good prospect for use in cancer gene therapy.  相似文献   

运动及芦荟干预对糖尿病大鼠肝脏SOD活性及基因表达的影响   总被引：4，自引：0，他引：4  

翁锡全  林文弢  黄森  陈晓彬 《广州体育学院学报》2006,26(5):92-96

探讨运动及芦荟干预对糖尿病大鼠肝脏SOD活性及其基因表达的影响,为糖尿病非药物疗法提供理论依据。方法:建立糖尿病大鼠模型并分别给予运动、芦荟及运动芦荟等干预,8wk后分别用分光光度法及RT-PCR技术检测大鼠肝脏MnSOD、CuZnSOD活性及其基因表达。结果:运动、芦荟及运动芦荟三种干预方法均能显著提高大鼠肝脏MnSOD、CuZnSOD活性及MnSOD基因表达,其中运动芦荟组对糖尿病大鼠肝脏SOD活性及基因表达最为明显。结论:说明运动结合芦荟干预更能提高糖尿病大鼠肝脏SOD的活性及基因表达水平,促进肝脏抗氧化作用效果显著。  相似文献   

构建基因专利池的利弊分析——以美国基因专利池为样本     

张羽 《太原大学学报》2014,(3):47-51

美国专利池的发展历程显示出专利池制度在众多领域有降低授权成本、减少恶性竞争、扩大产业联合的积极作用。但基因专利池同时面临着成本高、必要性低、容易造成垄断、保护无效专利等质疑。我国的基因产业亟待发展,我国的法律体系也允许基因专利的存在,对于基因专利池我们应该大胆尝试,同时吸取发达国家的教训,努力避免基因专利池的缺陷,而不应该在还没有试验的时候就畏首畏尾。  相似文献   

Adipose-derived stem cells transfected with pEGFP-OSX enhance bone formation during distraction osteogenesis     

Qing-guo LAI  Shao-long SUN  Xiao-hong ZHOU  Chen-ping ZHANG  Kui-feng YUAN  Zhong-jun YANG  Sheng-lei LUO  Xiao-peng TANG  Jiang-bo CI 《Journal of Zhejiang University. Science. B》2014,15(5):482-490

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