剑叶龙血树愈伤组织的诱导及其增殖培养研究     

杨晓虹  侯思名  和凤美  郭丽红  陈子牛 《昆明师范高等专科学校学报》2009,31(6):64-66

以西双版纳可提取药用活性成分“血竭”的剑叶龙血树为材料,进行愈伤组织的诱导及增殖培养,结果显示：外植体的消毒侧芽以75％乙醇浸泡2min＋0．1％升汞浸泡8min,花蕾以75％乙醇浸泡1．5min＋0．1％升汞浸泡8min,叶片以75％乙醇浸泡3min＋0．1％升汞浸泡12min效果较好;愈伤组织诱导以花蕾为外植体效果较好,且诱导与增殖的培养基为B5＋2．0mg/L NAA＋0．5mg／L6-BA．  相似文献   

采用低氧训练方法建立大鼠心肌组织血管新生动物模型     

邹志兵  郑澜  毛淑章 《北京体育大学学报》2010,(12)

目的:研究低氧训练对大鼠心肌组织血管生成的作用,从而构建大鼠心肌组织微血管新生的动物模型。方法:将健康雄性SD大鼠60只,按体重随机分为6组,每组10只,对照组,高住组,常练组,低住高练组,高住低练组,高住高练组。采用递增负荷跑台运动训练及低氧作为处理因素,建立大鼠低氧训练模型。采用免疫组织化学、显微图象分析对心肌组织毛细血管密度、光密度水平、表达面积进行计数和检测。结果:CD34能很好地标记心肌组织微血管,低氧训练对心肌组织微血管的生成效果理想。结论:采用低氧训练方法可以建立大鼠心肌组织血管新生的动物模型,为血管的生理性重建提供新的研究方法和途径。  相似文献   

黄褐毛忍冬组培技术研究   总被引：2，自引：0，他引：2  

白美发 《深圳职业技术学院学报》2009,8(3):57-60

选取黄褐毛忍冬的茎尖、1年生的茎段作为外植体,分别对表面灭菌的方法、取材部位、初代培养、增殖培养、生根培养等进行了研究．结果显示,在黄褐毛忍冬外植体的表面灭菌过程中,氯化汞是较好的表面灭菌剂;在外植体的取材上,应以茎尖为主;在初代培养过程中,激素6-BA在0．5—1．0mg／L,NAA在0．1—0．2mg／L或IBA在0．1mg／L,茎尖萌发都比较好;在增殖培养过程中,添加0．5mg／L 6-BA＋0．1mg／LNAA和0．5mg／L 6-BA＋0．1mg／L IBA的培养基最有利于组培苗的增殖;在生根培养时,使用IBA为1．5mg／L时的培养基更有利于幼苗生根．筛选了一个较好的黄褐毛忍冬组培快繁种苗方案,为黄褐毛忍冬产业发展提供了一种快速、优质的种苗繁殖途径．  相似文献   

脑组织移植治疗帕金森病的研究进展     

方琳 《岳阳职业技术学院学报》2009,24(3):91-94

脑组织移植后在免疫排斥、多巴胺能神经系功能的恢复及患者临床症状的减轻等方面.与移植多巴胺能细胞存活数量尤其是氨酸羟化酶细胞的数目密切相关.最新的研究成果对脑组织移植治疗帕金森病具有较强的指导意义.  相似文献   

Do habitual foot-strike patterns in running influence functional Achilles tendon properties during gait?     

Scott C. Wearing  I. S. Davis  T. Brauner  S. L. Hooper  T. Horstmann 《Journal of sports sciences》2013,31(23):2735-2743

ABSTRACT

The capacity of foot-strike running patterns to influence the functional properties of the Achilles tendon is controversial. This study used transmission-mode ultrasound to investigate the influence of habitual running foot-strike pattern on Achilles tendon properties during barefoot walking and running. Fifteen runners with rearfoot (RFS) and 10 with a forefoot (FFS) foot-strike running pattern had ultrasound transmission velocity measured in the right Achilles tendon during barefoot walking (≈1.1 ms^?1) and running (≈2.0 ms^?1). Temporospatial gait parameters, ankle kinematics and vertical ground reaction force were simultaneously recorded. Statistical comparisons between foot-strike patterns were made using repeated measure ANOVAs. FFS was characterised by a significantly shorter stance duration (?4%), greater ankle dorsiflexion (+2°), and higher peak vertical ground reaction force (+20% bodyweight) than RFS running (P < .05). Both groups adopted a RFS pattern during walking, with only the relative timing of peak dorsiflexion (3%), ground reaction force (1–2%) and peak vertical force loading rates (22–23%) differing between groups (P < .05). Peak ultrasound transmission velocity in the Achilles tendon was significantly higher in FFS during walking (≈100 ms^?1) and running (≈130 ms^?1) than RFS (P < .05). Functional Achilles tendon properties differ with habitual footfall patterns in recreational runners.  相似文献   

Do the effects of high intensity 40 km cycling upon left ventricular function and cardiac biomarker during recovery vary with time of day?     

F. Chan-Dewar  W. Gregson  G. Whyte  D. Gaze  J. Waterhouse  J. Wen 《Journal of sports sciences》2013,31(4):414-423

Abstract

Twelve healthy participants performed two identical high-intensity 40 km cycling trials (morning and evening) under controlled laboratory conditions. Echocardiograms and venous blood samples were collected before and after each exercise bout. Cardiac electro-mechanical-delay (cEMD) was measured as QRS-complex onset to peak systolic (S’) and early diastolic (E’) tissue velocities. Myocardial strain and strain rates were assessed in longitudinal, circumferential and radial planes at the left ventricular apex and base. Cardiac troponin I (cTnI) and N-terminal Pro-Brain Natriuretic Peptide (NT-proBNP) were assessed as biomarkers of cardiomyocyte damage and wall stress. cEMD was lengthened after both morning (S’: 160 ± 30 vs. 193 ± 27; E’: 478 ± 60 vs. 620 ± 87, P < 0.05) and evening (S’: 155 ± 29 vs. 195 ± 31; E’: 488 ± 42 vs. 614 ± 61, P < 0.05) trials. A reduction in peak S’ (morning: 6.96 ± 1.12 vs. 6.66 ± 0.89; evening: 7.09 ± 0.94 vs. 7.02 ± 0.76) was correlated with cEMD (r = ?0.335, P < 0.05). Peak longitudinal strain was reduced, atrial strain rates were sporadically increased in both trials post-cycling. cTnI was elevated in only two participants (0.04 µg · L^?1, 0.03 µg · L^?1), whilst NT-proBNP was below the clinical cut-off point in all participants. Prolonged-cycling resulted in a lengthening of cEMD, small changes in aspects of left ventricular deformation and sporadic increases in cardiac biomarkers. None of these effects were moderated by time-of-day.  相似文献   

兔骨膜成骨细胞的分离培养   总被引：2，自引：0，他引：2  

张丽君  丁焕文 《深圳职业技术学院学报》2004,3(2):34-37

采用植块法和分批消化法相结合的方法分离得到了兔骨膜成骨细胞,鉴定后进一步研究了其生长特性。结果表明:该法可以在短时间内得到足够数量的细胞且纯度较高(8d达1.8 × 105个／ml),其最终碱性磷酸酶(ALP)分泌量为(13.12±0.18)IU／ml,骨钙素(BGP)量约为(20±0.3)ng／ml。  相似文献   

"幸香"草莓组培快繁技术研究   总被引：2，自引：0，他引：2  

张铁  阙娥 《保山师专学报》2005,24(5):61-62

以幸香草莓为试材,对草莓葡匐茎茎尖生长点进行组织培养,筛选出适宜幸香草莓诱导分化培养基为MS BA0.50mg/L NAA0.10mg/L、继代增殖培养基为MS IBA0.10 mg/L BA0.50 mg/L、生根培养基为1/2MS IBA0.04 mg/L 糖15g/L.  相似文献   

微量元素及天然有机质对甜叶菊试管苗增殖和生长的影响     

崔广荣  何克勤  胡能兵  林平  张子学  王波 《安徽科技学院学报》2012,26(4):14-20

以甜叶菊试管苗为外植体,研究了MS培养基中微量元素对甜叶菊试管苗增殖、生根、生长及移栽等影响;同时也探讨了培养基中添加马铃薯汁、椰子汁、香蕉泥和水解乳蛋白等对试管苗增殖、生根、生长及移栽的影响。结果表明,微量元素对试管苗生长、增殖、生根产生重要作用,其中硼、锌、铜、铁的影响较为明显,尤其是锌元素是培养基中不可或缺的元素,它不仅影响试管苗的生长,而且对试管苗的移栽也产生较大影响,增加MS培养基中的锌元素的浓度,可促进试管苗的增殖、改善试管苗的质量;培养基中添加马铃薯汁、椰子汁、香蕉泥对试管苗的增殖具有一定的促进作用,其中香蕉泥能明显改进试管苗的质量,试管苗茎杆粗壮、生长旺盛,但添加水解乳蛋白却抑制试管苗的增殖和生长;在试管苗生根阶段,添加有机质作用不明显。  相似文献   

植物组织培养过程中的污染及其防治方法     

刘玲玲 《甘肃高师学报》2012,17(5):21-23

污染是植物组织培养过程中的常见问题之一,它严重影响了培养材料的正常发育和生长,并给科研和生产造成了不同程度的损失.主要对植物组织培养中出现的污染原因进行了分析,并提出了控制和降低污染率的有效方法,为科研和生产提供了一定的参考价值.  相似文献