重金属离子对猪血SOD的活力与构象影响     

谢晓兰  潘小芳  龚丽芬  陈婷 《泉州师范学院学报》2009,27(2):87-90

以猪血为材料,以超氧化歧化酶（简称SOD,EC1．15．1．1）为研究对象,经有机溶剂沉淀法得SOD酶液,测定SOD酶中铜锌含量比例．研究Hg^2＋、Pb^2＋、Cd^2＋等不同重金属离子对SOD活力与构象影响,结果表明：猪血SOD酶中含有等量的铜与锌．Hg^2＋、Cd^2＋对酶活力均表现为低浓度激活高浓度抑制作用．而Pb^2＋在考察的浓度范围内表现激活作用,荧光光谱显示三种重金属离子的存在均引起酶构象的改变．  相似文献   

小麦SOD基因的电子克隆及生物信息学分析     

孙双燕  李东方  张团  张胜利 《河南职业技术师范学院学报》2014,(4):7-10

以NCBI中的EST数据库为主要数据来源,以一系列生物信息学软件为工具,进行了超氧化物歧化酶（SOD）基因的电子克隆及生物信息学分析。结果表明：通过电子克隆方法获得了SOD基因的编码区全长序列,该预测的SOD蛋白质氨基酸序列与数据库中同类基因的蛋白质氨基酸序列具有极高的相似性,并且含有完整的保守结构域;电子克隆到的SOD基因编码的不是分泌蛋白,也不是膜蛋白,而是胞质蛋白。通过某个基因的一个EST序列采用电子克隆的手段进行基因全长的电子拼接是可行的。  相似文献   

高压氧对急性力竭性运动后血脂质过氧化物和超氧化物歧化酶代谢的影响   总被引：4，自引：0，他引：4  

金其贯  方明  黄叔怀  孙新荣 《浙江体育科学》1996,(6)

为了探讨ＨＢＯ对力竭性运动后机体氧自由基代谢的影响，让２０名体育系男大学生在自行车功率计上运动至力竭，然后随机分成ＨＢＯ组和对照组，他们分别在ＨＢＯ和自然状态下恢复约１２０Ｍｉｎ。在运动前、运动后和运动后２小时（恢复期）测定血清ＬＰＯ含量和ＳＯＤ活力。实验结果表明：短时间的力竭性运动使机体氧自由基的产生和清除处于一种高水平状态，并不破坏其平衡；适当的ＨＢＯ可以提高机体清除氧自由基的能力，有利于运动疲劳的恢复。  相似文献   

高产SOD乳酸菌培养条件的研究   总被引：1，自引：0，他引：1  

唐乾  唐咏  郑学仿 《大连大学学报》2004,25(2):67-69

本文从最适温度、初始pH、培养时间、培养基成份及培养方式等不同的方面对产SOD的乳酸菌发酵条件进行研究 找出最适发酵条件为:28～32℃,pH7.0,24h,静止培养  相似文献   

Growth rate, catalase and superoxide dismutase activities in rock carp (Procypris rabaudi Tchang) exposed to supersaturated total dissolved gas     

Liu XQ  Li KF  Du J  Li J  Li R 《Journal of Zhejiang University. Science. B》2011,12(11):909-914

Total dissolved gas supersaturation (TDGS) appears when the pressures of gases in a solution exceed the barometric pressures. TDGS is often caused by flood discharge at dams. It may lead to gas bubble disease (GBD) for fish and biochemical responses of selected fish and other aquatic organisms. The purpose of this study was to determine the impact of long-term TDGS levels on the growth and biochemical responses of rock carp (Procypris rabaudi Tchang) dwelling in the upper reaches of the Yangtze River. Three-year-old rock carp were exposed to TDGS levels at 100%, 104%, 108%, 112%, and 116% for 42 d. Samples were taken every 7 d after the start of the trial in order to determine catalase (CAT) and superoxide dismutase (SOD) activities in gill and muscle tissues. Samples were taken at Days 0 and 42 of exposure to determine growth rate. Little effect was found on growth rate in all treatment groups. SOD and CAT activities varied in different tissues, according to time of exposure and TDGS levels. The biochemical response of fish exposed to TDGS was more obvious in gill tissue than in muscle tissue. Surveys of SOD and CAT activities in different tissues offer important information about the effect of TDGS on the rare fish in the Yangtze River, and may help evaluate the risk to the aquatic eco-environment and aquatic ecosystem in the downstream of the Yangtze River.  相似文献   

运动中自由基的检测和适应性反应     

刘保华  李世昌 《河北体育学院学报》2009,23(3):72-75

回顾了运动中或运动后即刻自由基和相关活性氧的检测方法，在此基础上提出自由基（在中等增长水平）是一种引起运动适应的细胞内的信号分子，长时间的耐力运动和极大强度的运动所产生的大量自由基则毁坏细胞抗氧化荆的保护而导致组织损伤，这种情况下需要有效的蛋白质、脂质、DNA修复系统和充分的恢复才能够产生新的运动适应。  相似文献   

Study of Oxidative Stress in Vitiligo     

Jain A  Mal J  Mehndiratta V  Chander R  Patra SK 《Indian journal of clinical biochemistry : IJCB》2011,26(1):78-81

Vitiligo is an idiopathic, acquired, circumscribed, hypomelanotic skin disorder, characterized by milky white patches of different sizes and shapes. It is due to the destruction of melanocytes resulting in the absence of pigment production of the skin and mucosal surfaces. Oxidative stress has been implicated in pathophysiology of vitiligo. To study the activity of blood Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in vitiligo patients. A case–control study was conducted in which 100 patients were enrolled after written consent. 50 cases were of active vitiligo and 50 served as control (25 healthy control and 25 with stable vitiligo). SOD—In our study, among the active vitiligo cases 90% had high level of SOD and 10% had normal level of SOD. Among the stable vitiligo controls, 92% had normal level of SOD and 8% had low levels of SOD.The difference between active vitiligo cases and stable vitiligo control as well as with healthy control was statistically significant (P value < 0.05). GPx—Among the active vitiligo cases 74% had normal GPx levels, 22% had low and only 4% had high levels of GPx. Among the stable vitiligo controls, 64% had normal GPx levels, 16% had low, and 20% had high levels of GPx. The difference between active vitiligo cases and stable vitiligo control as well as with healthy control was statistically not significant (P value > 0.05). Our study shows that oxidative stress is involved in the pathophysiology of vitiligo, as indicated by the high levels of serum superoxide dismutase activity.  相似文献   

刺五加对甲醛致运动大鼠心肌氧化损伤的保护作用实验研究     

贾文娟 《思茅师范高等专科学校学报》2013,(6):13-18

目的：通过实验观察甲醛对运动大鼠心肌组织抗氧化系统的影响及服用刺五加后的作用,探讨甲醛对运动大鼠的心肌组织毒性及刺五加的保护作用。方法：采用人为模拟不同程度甲醛染毒环境进行动式染毒,甲醛剂量分别为0.8 mg/m3,2.4 mg/m3。每天染毒30 min,每周6次,连续4周,刺五加的灌胃剂量为104.17 mg/kg,测定运动大鼠心肌组织的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、过氧化氢酶（CAT）的活力和丙二醛（MDA）的含量。结果：染毒组与对照组相比,2.4 mg/m3剂量染毒运动组大鼠心肌组织 CAT、GSH-PX、SOD活性明显低于对照组（P〈0.01）,MDA含量明显高于对照组（P〈0.01）。服药染毒运动组与染毒运动组相比,0.8mg/m3剂量染毒服药组大鼠心肌组织CAT、GSH-PX、SOD活性明显高于染毒运动组（P〈0.01）,MDA含量明显低于染毒运动组（P〈0.01）。结论：甲醛可以降低染毒大鼠心肌组织抗氧化酶的活性,造成心肌组织氧化性损伤,而灌服中药刺五加可以有效缓解其毒性。  相似文献   

自由基损伤在甘油致家兔急性肾功能衰竭中的作用     

侯亚利  赵自刚  牛春雨  姜华  张静  樊贵 《河北北方学院学报(医学版)》2004,21(2):14-16

目的:观察甘油致急性肾功能衰竭(ARF)家兔自由基的变化,探讨自由基损伤在ARF发生中的作用及其意义.方法:30只家兔随机分为两组:50%甘油(10ml/kg.bw)肌肉注射复制家兔ARF模型,等量生理盐水代替甘油作为对照组(n均=15).分别应用改良TBA微量法、黄嘌呤氧化酶法测定血清及肾脏组织匀浆的丙二醛(MDA)水平、超氧化物歧化酶(SOD)活性的变化.结果:ARF组血清及肾脏组织匀浆的MDA水平高于对照组(P＜0.01),SOD活性显著低于对照组(P＜0.01).结论:自由基损伤在甘油致家兔ARF过程中具有重要作用.  相似文献   

绿柱虫γ-内酯二萜对脑缺血的保护作用     

许实波  项辉  钟永利  苏镜娱  曾陇梅 《中山大学学报论丛》1992,(3)

绿柱虫γ-内酯二萜(简称C)显著降低脑缺血组织脂质过氧化产物丙二醛的含量,提高超氧化物歧化酶活性,从而发挥其对脑缺血再灌注损伤之脑组织的保护作用。对脑组织的保护作用还与它能显著抑制血小板聚集有关。结果表明,C对缺血脑组织的保护作用可能在于它能拮抗Ca~(2 ),从而减轻再灌注时Ca~(2 )所造成的损伤。  相似文献