植物抗病的物质代谢基础   总被引：10，自引：0，他引：10  

夏启中  张明菊 《黄冈职业技术学院学报》2004,6(3):38-41

植物在其与痛原物相互关系长期进化过程中,通过各种代谢途径在体内可以形成各种抵抗病原物有关的物质。这包括植物凝集素、富含羟脯氨酸的糖蛋白、胼胝质、类黄酮和萜类植保素、过氧化物酶、几丁质酶(chitinease)和1,3-β-葡聚糖酶、病程相关蛋白和水杨酸等等。寄主植物细胞壁在感染病原菌后的木质化作用-木质素含量的增加是寄主植物抗性反应的一种特性,为阻止病原菌的进一步侵染提供保护圈。苯丙氨酸解氨酶(PAL),肉桂酸-4-羟化酶(CA4H)和4-香豆酸-CoA联结酶(4CL)是苯丙烷代谢途径的关键酶。这三个酶的活性在植物体内的变化与植物的抗病性存在一定的联系。水杨酸的Mr小,适宜于植物韧皮部中的长距离传输,被认为是植物诱导抗性的信号分子。  相似文献   

Mo(Ⅵ)-DBPF-PVA分光光度法测定尿蛋白     

衷明华 《韩山师范学院学报》2005,26(3):62-64

提出了一个借钼(VI)-4,5-二溴苯基荧光酮(DBPF)-聚乙烯醇(PVA)与蛋白质显色反应光度法测定微量尿蛋白的分析方法.在pH=2.0,牛血清蛋白能与Mo(VI)-DBPF- PVA形成紫红色络合物,最大吸收波长为570nm,牛血清蛋白含量0-5μg/mL范围内服从Beer定律.尿液中其它成份不干扰测定.用拟定的方法测定人尿蛋白,获得满意结果.  相似文献   

天然"豆皮"速溶饮料的研究     

谢朝兴 《Journal of Zhangzhou Technical Institute》2005,7(1):12-14,2

本文提出了一种天然"豆皮"速溶饮料生产新的技术与工艺方法.产品的理化指标及微生物学指标符合GB/T标准,含有18种氨基酸,其中9种为必需氨基酸.将其放入100℃沸水中冲泡1～3min,搅溶,即得清凉可口、保持原有豆皮的色、香、味等天然品质的"豆皮"速溶饮料.  相似文献   

适应性训练对骨骼肌肌力、骨架蛋白含量的影响   总被引：2，自引：0，他引：2  

马新东 《体育科学》2008,28(2):84-91

以雄性Wistar大鼠为研究对象,进行"3周、6周适应性离心运动训练"两种方式运动.其中3周适应性离心训练(渐进增加强度),每周5天训练,速度从20.0 m/min增加到32.5 m/min,每次20 min,-5°下坡间歇跑.6周适应性离心训练(相同强度),每周5天训练,前3周速度从20.0 m/min增加到32.5 m/min,后3周保持35.0 m/min,每次20 min,-5°下坡间歇跑.离心运动后不同时段和适应性训练后取大鼠后肢腓肠肌外侧头进行分析.6周适应性训练组进行一次性大强度离心运动,一次性离心运动实验为-16°下坡跑台跑,定量大负荷间歇性运动,跑速为26.8 m/min,运动5 min×10组,组间歇1 min.研究适应性训练后大鼠腓肠肌收缩力和拉断力和骨骼肌细胞骨架蛋白含量的变化特点;分析肌收缩力和拉断力与肌细胞骨架蛋白缺失的关系以及与延迟性骨骼肌损伤时血清酶升高的关系;通过观察大鼠经过适应性训练后,骨骼肌肌力及肌细胞基质蛋白含量是否会有适应性的变化,是否会因此提高对抗大强度离心运动所致骨骼肌损伤的能力.  相似文献   

黑糯玉米多糖的提取及脱蛋白工艺的比较     

张钟  杨丽 《安徽科技学院学报》2007,21(6):27-32

本文研究了黑糯玉米多糖的提取及两种脱蛋白分离工艺的比较.采用热水浸提、醇沉法提取黑糯玉米多糖,用两种改进的方法(氯仿-正丁醇法和三氯乙酸-正丁醇法)脱除蛋白质,以脱蛋白率为指标,通过正交实验L9(34)确定最佳脱蛋白分离工艺后,对比两种方法的最佳组合粗多糖得率及510nm处吸光值的差异.结果表明:三氯乙酸-正丁醇法比氯仿-正丁醇法的脱蛋白效果好,最佳水平脱蛋白率为70.69%,多糖得率仅为67.87%;氯仿-正丁醇法最佳水平脱蛋白率为51.39%,多糖得率为80.31%.本文建立的优化工艺可为黑糯玉米多糖的分离制备提供参考.  相似文献   

Overexpression of the steroidogenic acute regulatory protein increases the expression of ATP-binding cassette transporters in microvascular endothelial cells (bEnd.3)     

Yan-xia Ning  Shun-lin Ren  Feng-di Zhao  Lian-hua Yin 《Journal of Zhejiang University. Science. B》2010,11(5):350-356

Objective:To determine the effect of steroidogenic acute regulatory protein(StAR) overexpression on the levels of adenosine triphosphate(ATP)-binding cassette transporter A1(ABCA1) and ATP-binding cassette transporter G1(ABCG1) in an endothelial cell line(bEnd.3).Methods:The StAR gene was induced in bEnd.3 cells with adenovirus infection.The infection efficiency was detected by fluorescence activated cell sorter(FACS) and fluorescence microscopy.The expressions of StAR gene and protein levels were detected ...  相似文献   

Elevated high sensitive C-reactive protein and apelin levels after percutaneous coronary intervention and drug-eluting stent implantation     

Xin DU  Jun-ping KANG  Jia-hui WU  Qiang LV  Chao-shu TANG  Chang-sheng MA 《Journal of Zhejiang University. Science. B》2010,11(8):548-552

Objective:Percutaneous coronary intervention(PCI) triggers an acute inflammatory response,while sirolimus is known to have anti-inflammatory properties;the inflammatory system response to PCI after sirolimus-eluting stent placement remains unclear.The purpose of this study is to determine the changes in high sensitive C-reactive protein(hs-CRP) and apelin after PCI procedure and drug-eluting stent implantation in patients with and without reduced left ventricular systolic function.Methods:Forty-eight consecutive patients undergoing PCI at the Beijing Anzhen Hospital between July and September 2006 were recruited.Sirolimus-eluting stents were employed in all patients.Blood samples were drawn immediately before and 24 h after the procedure.Plasma hs-CRP and apelin levels were determined by enzyme immunoassay.Results:Paired t-test revealed a significant increase in both hs-CRP and apelin post-procedure(P=0.006 and P0.0001,respectively).Patients with reduced left ventricular ejection fraction(LVEF) had significantly lower baseline apelin levels compared to those with normal ventricular function [(46.8±10.8) vs.(72.0±8.4) pg/ml,P0.001].However,apelin increased to a level similar to the level of those with normal left ventricular systolic function 24 h after the PCI procedure [(86.7±11.6) vs.(85.1±6.1) pg/ml,P=0.72].Conclusions:hs-CRP and apelin levels increased after PCI and sirolimus-eluting stent implantation.Patients with impaired left ventricular systolic function had significantly lower baseline apelin levels,which increased significantly after PCI.  相似文献   

A study of the relationship between expression level of TRF1 protein and telomerase activity in human acute leukemia     

施继敏  黄河  陈巧芳  林茂芳 《Journal of Zhejiang University. Science. B》2006,7(2):154-158

INTRODUCTION Telomeres are distinctive DNA-protein struc-tures that cap the ends of linear chromosomes.It is very important to keep the chromosomes stabilization.Telomerase activity is closely linked to attainment of cellular immortality,a step in carcinogenesis,while lack of such activity contributes to cellular senes-cence.Telomerase is activated in more than85%ofmalignant tumors(Hiayma et al.,1997).Human te-lomeric repeat binding factor1(TRF1)is a telomere associated with proteins a…  相似文献   

Culture of Spirulina platensis in human urine for biomass production and O2 evolution     

Feng DL  Wu ZC 《Journal of Zhejiang University. Science. B》2006,7(1):34-37

Attempts were made to culture Spirulina platensis in human urine directly to achieve biomass production and O2 evolution, for potential application to nutrient regeneration and air revitalization in life support system. The culture results showed that Spirulinaplatensis grows successfully in diluted human urine, and yields maximal biomass at urine dilution ratios of 140-240. Accumulation of lipid and decreasing of protein occurred due to N deficiency. O2 release rate of Spirulina platensis in diluted human urine was higher than that in Zarrouk medium.  相似文献   

蛋白质芯片技术在自身免疫性疾病检测中的应用研究进展     

陈敏  方序 《科技通报》2012,28(5):84-88

自身免疫性疾病的检测诊断与发病机制非常复杂,蛋白质芯片技术成为研究以自身抗体为特征的自身免疫性疾病的快速、有效的工具,在定量检测和新标志物的发现中有着广泛的应用前景。蛋白质芯片是继基因芯片之后发展起来的一种后基因组研究技术,能够对样品蛋白进行高通量、高灵敏度、高特异性的分析,是蛋白质组学研究中强有力的工具。本文就蛋白质芯片及其在自身免疫系统疾病诊断与检测中的应用作一综述。  相似文献