兔角膜上皮特异性角蛋白的电泳分析          下载免费PDF全文

丁运华  葛瑞昌 《惠州学院学报》1999,19(4):32-35

本文提取兔角膜角蛋白,利用SDS-PAGE以及IEF／SDS-PAGE技术对其组成进行分析研究。结果表明,兔角幕上皮存在四种含量较高的特异性角蛋白,其分子量和等电点分别为：（Mr68kd,pI7．6）;（Mr64kd,PI5．4）;（Mr58kd,PI7．4）;（Mr55kd,PI5．2）．  相似文献   

高温不育水稻育性敏感期可溶性蛋白质的含量     

舒孝顺  陈良碧 《湖南师范大学教育科学学报》1999,(2)

可溶性蛋白质的含量按文［３］的方法进行测定,本文以常规品种紫壳作对照,分别测定了紫壳、高温敏感核不育材料１３５６Ｓ、衡农Ｓ—２在花粉母细胞形成期和花粉母细胞减数分裂期不同育性条件下的叶片和幼穗花药的可溶性蛋白质的含量,并进行了统计学分析——两因素方差分析（Ｆ检验）及平均数的Ｄｕｍｃａｎ多重比较．结果表明,在育性敏感的２个时期,１３５６Ｓ和衡农Ｓ—２不育株幼穗花药的可溶性蛋白质的含量极显著低于可育株可溶性蛋白质的含量,分别是可育株的４４．０％、４５．１％、３５．８％、４２．６％,不育株幼穗花药中可溶性蛋白质含量的严重不足,必将影响花粉的一系列生命活动,最终阻碍花粉的正常发育  相似文献   

纤维素酶及其水解稻草粉产生单细胞蛋白的研究     

粟学俐  贺飞 《荆门职业技术学院学报》1999,14(3):17-20

在不同的培养基条件下,对不同种类菌种的酶活进行比较,选出几种组合方式,对它们产生单细胞蛋白的能力进行分析,发现１７３蛋白质增量最多（７．１１％）。  相似文献   

对一幅插图的补充     

蔡晶 《中学生物教学》1998,(3)

对一幅插图的补充九年义务教育三年制初级中学教科书《生物》第一册（上）Ｐ．１９图Ⅰ—６是细胞分裂过程图。笔者在教学中感到这幅图由于对细胞核的分裂未加体现,因而略显欠缺。书中原图如下：图１书中对图的说明是这样的：细胞分裂就是一个细胞分成两个细胞。简单地说...  相似文献   

亚低温有氧运动联合干预NAFLD的可能生物学机制述评     

秦智  李彩洁  肖国强 《体育学刊》2010,17(2)

综述了非酒精性脂肪肝病(NAFLD)致病机制、有氧运动诸多雏持稳态机制与低温生物学综合效应研究概况.科学猜测与述评亚低温与有氧运动联合干预NAFLD的可能生物学机制是亚低温诱导机体生成应激蛋白发挥综合生物效应与有氧运动促机体抗氧化应激、抑制线粒体凋亡、改善内质网应激与胰岛素抵抗,抗炎症作用共同实现NAFLD病理性的可逆改善.  相似文献   

大鼠间歇训练对海马c—fos蛋白表达的影响     

于芳  张安民  李楠 《体育科学研究》2010,14(2):78-81

为了观察大鼠间歇训练对海马c—fos蛋白表达的影响。将雄性SD大鼠30只,随机分为对照组（5只）、间歇训练组（25只）。建立大鼠间歇游泳训练模型,采用ABC免疫组织化学法,观察大鼠海马c—fos阳性神经元细胞表达的变化（即c-fos蛋白表达变化）,并用图像分析系统和统计学软件进行图像和数据分析。结果表明：训练后大鼠海马不同区域c-fos阳性细胞表达密度明显增高,与对照组相比均有显著性差异（P〈0．01）;CA2区c-fos阳性细胞表达迅速而明显,训练后0．5h即达峰值,而后回落;CA3区c—fos阳性细胞表达最为密集,训练后1h达到高峰,而后快速回落;CA1区c-fos阳性细胞表达相对较少,1h达到高峰,而后缓慢下降。结论：大鼠间歇训练对海马不同区域c—fos蛋白表达有影响,具有时效性;海马不同区域c—fos蛋白表达在密度、时相上存在明显差异,这可能与海马不同区域对缺血、缺氧的耐受能力有关。  相似文献   

Verification of Atellica 1500 and comparison with Iris urine analyser and urine culture     

Ana Nikler  Helena i ak  Danijela Bejuk  Vanja Radi&#x;i&#x; Biljak  Ana-Maria &#x;imundi&#x; 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2022,32(1)

IntroductionThe aims of study were to assess: 1) performance specifications of Atellica 1500, 2) comparability of Atellica 1500 and Iris, 3) the accuracy of both analysers in their ability to detect bacteria.Materials and methodsCarryover, linearity, precision, reproducibility, and limit of blank (LoB) verification were evaluated for erythrocyte and leukocyte counts. ICSH 2014 protocol was used for estimation of carryover, CLSI EP15-A3 for precision, and CLSI EP17 for LoB verification. Comparison for quantitative parameters was evaluated by Bland-Altman plot and Passing-Bablok regression. Qualitative parameters were evaluated by Weighted kappa analysis. Sixty-five urine samples were randomly selected and sent for urine culture which was used as reference method to determine the accuracy of bacteria detection by analysers.ResultsAnalytical specifications of Atellica 1500 were successfully verified. Total of 393 samples were used for qualitative comparison, while 269 for sediment urinalysis. Bland-Altman analysis showed statistically significant proportional bias for erythrocytes and leukocytes. Passing-Bablok analysis for leukocytes pointed to significant constant and minor proportional difference, while it was not performed for erythrocytes due to significant data deviation from linearity. Kappa analysis resulted in the strongest agreements for pH, ketones, glucose concentrations and leukocytes, while the poorest agreement for bacteria. The sensitivity and specificity of bacteria detection were: 91 (59-100)% and 76 (66-87)% for Atellica 1500 and 46 (17-77)% and 96 (87-100)% for Iris.ConclusionThere are large differences between Atellica 1500 and Iris analysers, due to which they are not comparable and can not be used interchangeably. While there was no difference in specificity of bacteria detection, Iris analyser had greater sensitivity.  相似文献