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71.
血滤器的中空纤维聚砜膜的形态学观察方法的探讨和评价   总被引:1,自引:0,他引:1  
提出了2种用于构建生物人工肾小管的中空纤维膜聚砜膜表面的观察的新方法.采用免疫荧光染色(immunofluorescence staining,IF)和原子力显微镜(atomic force microscopy,AFM)对中空纤维聚砜膜内和外壁进行观察研究.实验表明免疫荧光染色方法简便快捷,图象清晰,AFM的分辨率高,图象清晰,是除扫描电镜(scanning electronic micmscope,SEM)外的两种观察生物人工肾小管的简便可靠方法.普通显微镜观察不能观察到中空纤维聚砜膜形态,AFM简便,图象清晰,分辨率较高,并且可以观察立体三维形态,实验结果显示IF和AFM是观察生物人工肾小管的中空纤维膜聚砜膜表面的观察的新方法,并且简便使用,分辨率高,能观察三维结构.  相似文献   
72.
近光学衍射场的压缩效应研究   总被引:1,自引:0,他引:1  
给出了近光学衍射场的测不准关系式,得出了该衍射场具有的压缩效应与压缩真空态下介观RLC电路中存在的量子效应是等效的结论。对近场光学扫描高分辨显微镜的研究和微型电子器件及电路的设计均具有实际指导意义。  相似文献   
73.
运动心脏重塑的发生与转归   总被引:5,自引:0,他引:5  
为了进一步探讨运动心脏结构与功能的发生、转归及其与病理心脏的本质差异,作者通过动物实验模拟运动心脏,观察了经过12周耐力训练及完全停止运动训练8周后心脏重量和超微结构的变化,并应用激光扫描共聚焦显微镜与新一代钙荧光指示剂Fluo—3/AM负载方法对心肌活细胞内具有生物活性的游离钙的动态变化进行了研究。结果表明,运动心脏超微结构和胞内钙产生了良好的适应性改变,心肌收缩时收缩结构钙可获得量增多,构成运动心脏心肌收缩性、氧化代谢及自分泌功能增强的重要细胞机制。完全停训后,运动心肌细胞内游离钙,心房特殊囊粒,心肌线粒体及毛细血管结构适应性改变的消退证实运动心脏结构与功能改变具有可恢复性,区别于病理心脏。  相似文献   
74.
In mainland China, histology and embryology (HE) are taught in one course as an essential component of medical curricula. The effectiveness of HE courses directly affects the quality of medical students. To determine the present situation and changes in HE teaching in Chinese medical schools, a nationwide survey was conducted among the HE departmental leaders. In total, 66 responses were included in the study, representing prominent Chinese mainland medical schools. The results revealed that most HE teachers have medical educational backgrounds; an increasing number of teaching staff with PhDs have joined the teaching staffs. A range of 71 to 90 HE curriculum contact hours is predominant. The ratio of theory to practice for HE contact hours is 1:1 at half of the surveyed medical schools. The numbers of students in each laboratory are less than 30 and from 31 to 60 at 23 and 36 medical schools, respectively. Virtual microscopy is employed in 40% of the surveyed medical schools. Didactic teaching is the most common strategy, although new teaching approaches are being employed gradually. During the past 20 years, both the total number of HE teachers and the number of HE teachers with medical educational backgrounds have been reduced in at least half of the surveyed schools. A total of 83.33% of the surveyed schools have reduced their HE contact hours. Almost half of the Chinese medical schools remained unchanged in both their ratio of theory to practice and the number of students in each laboratory. The data derived from this study help to understand the development of the HE discipline at Chinese medical schools.  相似文献   
75.
76.
《文物保护研究》2013,58(2):97-106
Abstract

The Danish sculptor Anne Marie Carl-Nielsen (1863–1945) often prepared three-dimensional models in wax before producing her works of art. The Carl Nielsen Museum in Odense keeps a unique collection of around 200 of the artist's fragile wax models. In 2008 the entire collection was examined, documented, X-ray radiographed, photographed, and conserved. Analyses of five figurines by attenuated total reflectance-Fourier transform infrared spectroscopy, optical microscopy, and scanning electron microscopy-energy dispersive X-ray spectroscopy showed two different kinds of wax mixtures, one containing beeswax and potato or maize starch as filler, and the other consisting of gypsum, zinc stearate, and a greasy substance. During the examination a specific deterioration phenomenon was noticed in the areas where the wax models had been strengthened with internal metal armatures. A chemical reaction between the wax mixture and the copper containing armature has caused an intense greenish colouring of the wax as well as creating a soft and almost liquid consistency and formation of copper(II) carboxylate salts. Based on an original recipe from Anne Marie Carl-Nielsen, a replica of one of the wax mixtures was produced and examined with respect to its infrared spectrum and its modelling properties.  相似文献   
77.
Wood identification is a basic information that should interest any wooden artefact. This typically involves invasive sampling, but sometimes sampling is unattainable either because of the object typology or because it is difficult to obtain authorizations. In the present study, reflected light microscopy potential as a non-invasive identification tool for wooden cultural artefacts is assessed on a number of marquetry furniture and small wood carvings. In more than one half of the 13 examined cases accurate wood identification was possible, while the remaining cases yielded information of diagnostic value, making it possible to exclude several potential candidate species. In a number of cases the use of optical filters improved the visibility of character states. Shape and orientation of surfaces influenced the visibility of microscopic characters. The study confirms that reflected light microscopy is a valuable tool for non-invasive wood identification. In many cases it is able to support accurate identification, in others it can anyway provide important information, useful to help decision about supposed species, or to limit the invasiveness of possible further analysis by addressing them on specific features.  相似文献   
78.
表界面分子自组装与调控是分子科学研究的前沿课题之一。研究表界面分子组装与调控有助于深入理解对分子吸附、分子间相互作用和吸附组装结构等科学问题,有助于发展新型纳米材料,制备新型纳米器件。本文以本课题组近年研究工作为主,介绍利用扫描隧道显微技术研究固体表面分子吸附组装以及如何调控分子组装结构的部分结果,包括分子吸附,主客体分子组装,组装结构调控等内容,并分析展望了该研究领域的发展趋势。  相似文献   
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Increasing enrollment in post‐secondary institutions across North America, along with an increase in popularity of and demand for distance education is pressuring institutions to offer a greater number and variety of courses online. A fully online laboratory course in microscopic anatomy (histology) which can be taught simultaneously with a face‐to‐face (F2F) version of the same course has been developed. This full year course was offered in the Fall/Winter (FW) terms in both F2F and online formats. To ensure that the online course was of the same quality as the F2F format, a number of performance indicators were evaluated. The same course, offered exclusively online during the summer with a compressed time frame, was also evaluated. Senior undergraduate students self‐selected which version of the course they would enroll in. Course assessment outcomes were compared while incoming grades were used as a predictor for course performance. There were no significant differences between the incoming grades for the F2F FW and Online FW courses; similarly, there were no significant differences between outcomes for these formats. There were significant differences between the incoming grades of the F2F FW and Summer Online students. However, there were no significant differences among any of the outcomes for any of the formats offered. Incoming grades were strong, significant predictors of course performance for both formats. These results indicate that an online laboratory course in microscopic anatomy is an effective format for delivering histology course content, therefore giving students greater options for course selections. Anat Sci Educ 6: 246–256. © 2013 American Association of Anatomists.  相似文献   
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