模拟3500m不同低氧训练模式对大鼠力竭运动后心肌线粒体自由基代谢及呼吸链功能的影响     

李洁  邢良美 《上海体育学院学报》2012,(1):51-55

目的:探讨4种低氧训练模式对大鼠力竭运动后心肌线粒体自由基代谢及呼吸链功能的影响。方法:将雄性Wistar大鼠随机均分为5组,即低住低训组(LoLo)、高住高练组(HiHi)、高住低训组(HiLo)、低住高练组(LoHi)及高住高练低训组(HiHiLo)。以当地环境为基点作为常氧环境,采用低压氧舱模拟低氧环境。依实验方案,各组大鼠分别在常氧或/和低氧环境中居住及递增负荷训练5周,每周训练6 d。各组大鼠在最后1次训练后,在常氧环境中恢复3 d,力竭运动后即刻取心肌样本。用差速离心法提取心肌线粒体,分别测定丙二醛含量、超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶(catalase,CAT)活性及呼吸链酶复合体I～IV(CI～IV)活性。结果:与LoLo组相比,心肌组织中HiHi组GSH-Px及CAT活性均显著升高,HiLo组CAT活性显著升高,LoHi和HiHiLo组SOD、GSH-Px及CAT活性均显著升高,4种低氧训练模式MDA含量均显著降低。与LoLo组相比,HiHiLo组心肌线粒体SOD活性显著升高,HiHi、HiLo和HiHiLo组MDA含量均显著降低。与LoLo组相比,HiHi组心肌线粒体CIV活性显著升高,HiHiLo组CI、CII和CIV活性均显著提高,HiHi、LoHi及HiHiLo组CIII活性均显著降低。结论:HiHiLo可能是较好的低氧训练模式。  相似文献   

Protection of salvianolic acid B for human endothelial cells against hydrogen peroxide-induced oxidative damage     

张俊刚  赵广荣  刘金玲  季祥武 《天津大学学报(英文版)》2009,15(6):434-439

Salvianolic acid B(Sal B) is an active component of traditional Chinese medicine Salvia miltiorrhiza and is used to treat vascular diseases. To better understand its mechanism, the antioxidant capacities of Sal B was evaluated with human endothelial cells under oxidative stress. Human endothelial cells were pretreated with Sal B for 12 h followed by hydrogen peroxide for another 12 h. Production of reactive oxygen species (ROS), activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), and concentration of glu-tathione were measured: Protective effect of Sal B on the endothelial cells from hydrogen peroxide-induced damage ' was observed, and ROS production in the cells was found significantly inhibited. Sal B remarkably enhanced the activities of antioxidant enzymes SOD, CAT and GPX. Furthermore, Sal B up-regulated the intracellular glutathione concentration. The results indicate that Sal B protected endothelial cells from oxidative stress by improving the redox status of the cells through enhancing the antioxidant enzyme activities and increasing the reductive glutathione concentration after the oxidative challenge.  相似文献   

Oxidative stress in elite athletes training at moderate altitude and at sea level     

Josefa León-López  Carmen Calderón-Soto  Matías Pérez-Sánchez  Belén Feriche  Xavier Iglesias  Diego Chaverri 《European Journal of Sport Science》2018,18(6):832-841

Using a controlled parallel group longitudinal trial design, we investigated the effects of different training interventions on the prooxidant/antioxidant status of elite athletes: living and training at moderate altitude for 3 (Hi-Hi3) and 4 weeks (Hi-Hi), and for 4 weeks too, living high and training high and low (Hi-HiLo) and living and training at sea level (Lo-Lo). From 61 swimmers, 54 completed the study. Nitrites, carbonyls, and lipid peroxidation (LPO) levels were assessed in plasma. Enzymatic antioxidants glutathione peroxidase (GPx) and glutathione reductase (GRd), and non-enzymatic antioxidants total glutathione (GST), reduced glutathione (GSH) and oxidized glutathione (GSSG) were analysed in the erythrocyte fraction. At the end of the intervention, nitrites levels were similar in all altitude groups but higher than in the Lo-Lo controls (P?=?.02). Hi-HiLo had greater GPx activity than Hi-Hi and Hi-Hi3 during most of the intervention (P?≤?.001). GRd activity was higher in Lo-Lo than in Hi-Hi at the end of the training camp (P?≤?.001). All groups showed increased levels of LPO, except Lo-Lo, and carbonyls at the end of the study (P?≤?.001). Training at altitude for 3 or 4 weeks drives oxidative stress leading to cellular damage mainly by worsening the antioxidant capacities. The GSSG/GSH ratio appears to be related to perceived exertion and fatigue. The stronger antioxidant defence showed by the Hi-HiLo group suggests an inverse relationship between redox alterations and performance. Further studies are required to investigate the role of oxidative stress in acclimatization, performance, and health.  相似文献   

Evaluation of hypoglycemic and antioxidant effect of<Emphasis Type="Italic">Ocimum sanctum</Emphasis>     

Jyoti Sethi  Sushma Sood  Shashi Seth  Anjana Talwar 《Indian journal of clinical biochemistry : IJCB》2004,19(2):152-155

Ocimum sanctum leaves have been traditionally used in treatment of diabetes mellitus. Dietary supplementation of fresh tulsi leaves in a dose of 2 gm/kg BW for 30 days led to significant lowering of blood glucose levels in test group. Intake ofOcimum sanctum also led to significant increase in levels of superoxide dismutase, reduced glutathione and total thiols, but marked reduction in peroxiodised lipid levels as compared to untreated control group. The leaves were found to possess both superoxide and hydroxyl free radical scavenging action. The present observations establish the efficacy ofOcimum sanctum leaves in lowering blood glucose levels and antioxidant property appears to be predominantly responsible for hypoglycemic effect.  相似文献   

余甘子抗氧化成分的提取与抗氧化活性研究   总被引：1，自引：0，他引：1  

林素英  黄旭心  蔡财木  姜伟平 《莆田学院学报》2011,18(2):71-73,93

以乙醇为溶剂,超声波辅助提取余甘子果实的抗氧化成分并作用于花生油,对提取工艺及提取物对花生油的抗氧化活性进行了研究。正交实验的结果表明最佳提取工艺条件为:固液比为1:20、超声波处理60min、乙醇质量分数为50%;余甘子提取物能明显降低花生油过氧化值,常温下有良好的热稳定性与抗氧化性能。  相似文献   

抗氧化剂对鲁米诺—亚硫酸钠化学发光体系发光强度的影响     

高宏峰 《晋城职业技术学院学报》2011,4(4):47-51

亚硫酸盐对动植物的毒作用与其诱导体内产生过量自由基和活性氧有关。由鲁米诺和亚硫酸盐组成的简明而稳定的化学发光体系,在达到最佳发光强度时观察到发光强度可以被超氧化物歧化酶（SOD）、抗坏血酸（Vc）、谷胱甘肽（GSH）等拮抗超氧阴离子O2.自由基的抗氧化剂所抑制,说明在此体系中有超氧阴离子产生,鲁米诺的氧化是由这些超氧阴离子引起的,实验表明亚硫酸盐易与溶解氧反应产生O2.,而抗氧化剂对亚硫酸盐的毒作用有一定的抑制效果。  相似文献   

小叶女贞果实抗氧化活性研究   总被引：1，自引：0，他引：1  

杨再波  吴勇刚 《黔南民族师范学院学报》2011,31(3):47-49

以乙醇为溶剂提取小叶女贞果实,经减压浓缩后,依次用乙酸乙酯和正丁醇进行萃取。用DPPH自由基的清除能力来评价两个部位的抗氧化活性。结果表明,乙酸乙酯部位浓度为500μg/mL时自由基清除率大于75%,IC50=302.818μg/mL,正丁醇部位浓度为300μg/mL时自由基清除率大于75%,IC50=189.241μg/mL。正丁醇部位抗氧化能力比乙酸乙酯部位更好,是乙酸乙酯部位的1.6倍。  相似文献   

玉米花粉多糖对自由基清除作用的研究     

李云捷  陈列芹  邓家庆 《德州学院学报》2011,27(4):52-55

对羟基自由基和超氧阴离子自由基的清除试验表明,不同玉米花粉多糖级分对二者的清除作用均具有较好的量效关系,且复合一元二次方程模型;清除羟基自由基需要PPM、PMA、PMB及PMC-1的半数浓度分别为104.99μg/mL、146.98μg/mL、172.56μg/mL和149.98μg/mL;清除超氧阴离子需要PP M、PMA、PMB及PMC-1四者的半数清除浓度依次为267.65μg/mL、290.56μg/mL、307.10μg/mL和268.59μg/mL.且玉米花粉多糖中抗氧化活性最强的是PPM和PMC-1.  相似文献   

薏米多酚的优化提取及其抗氧化研究     

李玲  王益志  闫旭宇 《湘南学院学报》2015,(2)

利用乙醇提取薏米多酚类物质,研究其提取工艺条件及抗氧化性.结果表明,影响多酚类物质得率的的强弱因素依次为：料液比( D)＞乙醇浓度( B)＞提取温度( A)＞提取时间( C);最佳提取条件为：温度为40℃、乙醇浓度为70％、提取时间为2h、料液比为1：12,即最佳组合条件为A1 B3 C3 D3,此时薏米多酚提取率达到最大化,多酚得率为48.58mg/100g.同时,抗氧化性试验表明薏米中多酚类物质有较好的抗氧化性,但相对于TBHQ、维生素C抗氧化性较弱.  相似文献   

Effect of ketoacids on H<Subscript>2</Subscript>O<Subscript>2</Subscript> induced cataract     

R. M. Jain  P. M. Bulakh 《Indian journal of clinical biochemistry : IJCB》2003,18(1):91-95

Cataract is one of the leading causes of visual disability often leading to blindness in the elderly population. One of the causes is oxidation of proteins present in lens, by hydrogen peroxide (H₂O₂). In the present study 100 goat lenses were analyzed to determine the protective efficacy of ketoacids, against the oxidative insult by H₂O₂. The ketoacids used were (pyruvate, alpha ketoglutarate and oxaloacetate), that are constantly produced endogenously. The lenses were incubated as control and experimental groups in TC-199 media for 72 hrs. H₂O₂ concentration of 10mM was used to induce cataract. The biochemical parameters measured were levels of malondialdehyde (MDA), a lipid peroxidation product and activity of glutathione peroxidase (G-Px), an enzymatic antioxidant. The results showed a significant increase in the levels of MDA and significant decrease in the activity of G-Px in the cataractous lenses as compared to control. After addition of ketoacids (pyruvate (10mM), alpha ketoglutarate (20mM) and oxaloacetate (20mM)) separately, the levels of MDA decreased significantly and the activity of G-Px increased significantly. The results suggest that the ketoacids can be very promising antioxidants for the treatment of cataract. They may also be useful in treating other disabilities related to acute and chronic oxidative stress.  相似文献