Pro-oxidant and anti-oxidant status in patients of sickle cell anaemia     

Jyoti Titus  Suresh Chari  Madhur Gupta  Nitin Parekh 《Indian journal of clinical biochemistry : IJCB》2004,19(2):168-172

The role of oxidant damage to red cells in sickle cell anaemia has been of interest in recent years. Although, available reports suggest that sickle cell erythrocytes are susceptible to endogenous free radical mediated oxidant damage there remains discrepancy in the status of antioxidant enzymes and antioxidant vitamins in these patients. In view of this, 107 cases of sickle cell anaemia (36 ‘SS’ and 71 ‘AS’ pattern—as confirmed by haemoglobin electrophoresis) were subjected to analysis of malondialdehyde, ascorbic acid, superoxide dismutase and albumin. The results were compared with 54 age and sex matched healthy controls. The results indicate a marked increase in lipid peroxidation and superoxide dismutase levels in both ‘SS’ and ‘AS’ types of sickle cell anaemia as compared to controls. Although no difference was observed in the levels of albumin in these groups the levels of ascorbic acid were significantly depleted in sickle cell anaemia patients. The results are indicative of enhanced lipid peroxidation along with imbalance in the pro-oxidant and antioxidant status in patients of sickle cell anaemia.  相似文献   

抗氧化因子与天然抗氧化剂研究综述   总被引：12，自引：0，他引：12  

乔凤云  陈欣  余柳青 《科技通报》2006,22(3):332-336

近十几年来,抗氧化作用因在医疗、保健、食品保鲜等领域内占有越来越重要的地位而引起人们的普遍关注。本文综述了抗氧化作用的机理、生物学意义,抗氧化物质的种类及天然抗氧化剂的资源及应用现状,并对该领域作了展望。  相似文献   

红车轴草种子脂溶性成分GC-MS分析及抗氧化活性研究     

郑鹏  俞君如  白成科 《西安文理学院学报》2008,11(2):24-28

采用索氏提取法提取红车轴草种子中的脂溶性成分,进行甲酯化处理后用气相色谱-质谱联用技术（GC-MS）分离和鉴定脂溶性成分的组成和含量,同时运用N,N-二苯基三硝基苯肼自由基（DPPH）清除法和β-胡萝卜素漂白法对红车轴草种子脂溶性成分的抗氧化活性进行了初步研究.GC-MS法研究分析表明,红车轴草种子中共分离鉴定出6种脂溶性成分,包括亚油酸（29.64%）、（Z）-6-十八烯酸（14.60%）、棕榈酸（5.91%）、硬脂酸（3.24%）、三十烷酸（0.49%）和草酸葵基-丙基酯（0.17%）.抗氧化活性分析表明,红车轴草种子脂溶性成分对DPPH的自由基清除率IC50为17.97 mg/mL,0.04和0.08 mg/mL的β-胡萝卜素漂白法的抗氧化速率分别为29.9%和32.1%,均显著低于阳性对照BHT（96%）,说明红车轴草种子脂溶性成分的抗氧化活性较低.以上结果将对提取红车轴草种子脂溶性成分分析、提取分离和综合开发利用具有一定的参考价值.  相似文献   

小麦幼苗抗冻蛋白的分离及其抗氧化活性的研究     

郭丽红  仝向荣  王德斌  陈善娜 《昆明师范高等专科学校学报》2006,28(4):71-74

通过低温处理小麦幼苗来诱导抗冻蛋白的表达，采用DEAE-52和丙烯葡聚糖S300柱层析的方法分离了小麦幼苗中的抗冻蛋白，并对所分离的蛋白进行了SDS-PAGE分析，而且对其抗氧化活性也进行了初步研究，结果表明：低温处理可以诱导小麦幼苗抗冻性，从小麦幼苗中分离到一类热稳定的抗冻蛋白，其分子质量约为20～34ku，当浓度为10g／L时，测得其热滞活性为0．3℃．此类蛋白对羟基自由基活性有一定的抑制作用，表明它具有抗氧化活性．  相似文献   

双酶法水解寻氏肌蛤蛋白制备抗氧化肽的研究     

孔美兰  金榕  王佳虹 《教育教学论坛》2019,(29):106-108

文章以寻氏肌蛤为原料制备抗氧化肽,研究木瓜蛋白酶—复合蛋白酶双酶法酶解寻氏肌蛤的工艺条件。以1,1-二苯基-2-苦肼基(DPPH)自由基清除率为测定指标,通过单因素和正交试验确定双酶法制备抗氧化肽的最佳条件。结果显示:pH6.0,酶比例1∶1,[E]/[S]8%,酶解温度60℃,酶解时间6.5h为最佳酶解条件。验证试验表明,在此条件下,抗氧化肽对DPPH自由基清除率为68.70%。  相似文献   

Total antioxidant capacity and lipid peroxidation in liver transplantation     

Vaishali N. Thorat  Adinath N. Suryakar  Pradeep Naik  Bipin M. Tiwale 《Indian journal of clinical biochemistry : IJCB》2009,24(1):102-104

Liver transplantation is an accepted therapy for chronic liver disease patients. These patients generally have low levels of fat soluble vitamins, which have important antioxidant roles. Therefore, this study was undertaken to investigate whether such patients had evidence of antioxidant depletion and increased lipid peroxidation before transplant and whether the subsequent ischemia and reperfusion encountered during liver transplantation have any effect on antioxidant levels and lipid peroxidation. We assessed plasma total antioxidant capacity and serum lipid peroxide in 12 patients undergoing liver transplantation and equal numbers of healthy subjects. We found that before reperfusion, antioxidant levels were significantly decreased along with significantly elevated lipid peroxidation levels as compared with healthy controls (P<0.001). On reperfusion of the liver graft, further declined values of total antioxidant accompanied with highly elevated lipid peroxidation were seen than those of pre-reperfusion samples (P<0.001). This data shows that patients undergoing liver transplant have lowered antioxidant defenses and evidence of free radical damage, which compound the additional insult of reperfusion injury. Therefore antioxidant therapy in these patients before transplantation may ameliorate the effects of reperfusion.  相似文献   

豆豉生理功能的研究进展   总被引：1，自引：0，他引：1  

李华  冯凤琴  沈立荣  李铎 《科技通报》2009,25(4):498-502

豆豉,这种传统的发酵豆制品,一直以来都是受人们欢迎的调味品.近期的研究表明,豆豉具有多种生理功能,如抗氧化能力、抗血栓、降血糖和降血脂作用,因此,豆豉有望成为一种功能性食品.  相似文献   

运动中自由基生成: 线粒体的作用   总被引：6，自引：0，他引：6  

吉力立 《天津体育学院学报》2000,15(1):1-6

正常细胞的一生中可以产生活性氧基团（ＲＯＳ）。生理状态下,线粒体呼吸链是ＲＯＳ主要来源,其中不多于线粒体总氧耗的５％用于产生ＲＯＳ。研究已经表明,在剧烈有氧运动时骨骼肌ＲＯＳ生成增多,但是线粒性体在ＲＯＳ生成增多中的量化作用仍不十分清楚。大强度运动可引起线粒体出现各种形式的氧化损伤,如脂质过氧化、蛋白质氧化、氧化还原状态紊乱及酶的失活等。这些生物化学的修饰作用将导致以呼吸链缺损和解偶联为标志的线粒  相似文献   

Hyperlipidemia, increased lipid peroxidation and changes in antioxidant enzymes, Na+−K+-ATPase in erythrocytes of type 2 diabetic patients in andhra pradesh     

R. Giri  M. M. Kesavulu  B. Kameswara Rao  V. Ramana  Ch. Appa Rao 《Indian journal of clinical biochemistry : IJCB》1999,14(2):168-175

Plasma levels of lipids, lipoproteins and lipid peroxides and erythrocyte Na⁺−K⁺ ATPase, Mg²⁺ATPase and antioxidant enzymes were measured in type-2 diabetic patients. A significant decrease in Na⁺-K⁺-ATPase activity was observed in diabetic patients which was negatively correlated with blood glucose and lipid peroxides, while the Mg²⁺-ATPase activity was increased. In the diabetic subjects the plasma concentrations of Na⁺ and K⁺ were increased where as erythrocyte levels of Na⁺ were increased and K⁺ were decreased. Hyperlipidaemia and increased levels of lipid peroxides were observed in the diabetic subjects. There was a significant increase in erythrocyte catalase activity in diabetics which positively correlated with their lipid peroxides. There was no change in GPx activities between controls and diabetics.  相似文献   

联合抗氧化剂对一次大强度运动后运动员T-AOC、SOD、GSH-Px及MDA的影响   总被引：3，自引：0，他引：3  

张慧  苏全生 《北京体育大学学报》2008,31(4):483-485

目的:探讨一次性大强度离心运动以及联合抗氧化剂干预对运动员抗氧化能力、氧化应激以及脂质过氧化损伤的影响;方法运动员16名,随机均衡的分为对照组、实验组,受试对象服药2周后,进行一次性大强度离心跑台运动,分时相测定SOD、MDA、T-AOC、GSH-Px。结果:实验组服药后T-AOC水平即开始升高,非常显著高于服药前水平(P<0.01),运动后实验组各时相均显著高于对照组(P<0.01-0.05);SOD活性,实验组服药后有所升高,运动后即刻显著(P<0.05),24 h非常显著性(P<0.01)高于对照组,48 h高于对照组;实验组GSH-Px活性,运动后即刻显著(P<0.05)、48 h非常显著(P<0.01)高于对照组;运动后两组运动员MDA水平均呈升高趋势,实验组运动后24h显著(P<0.05)4、8 h非常显著(P<0.01)低于对照组,其他各时相升高幅度均低于对照组。结论:联合抗氧化剂能有效提高机体抗氧化能力,减轻大强度运动引起的氧化应激、脂质过氧化损伤。  相似文献