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INTRODUCTIONAfterthewholericegenomewassequencedsuccessfully,moreandmoregenescanbepredictedwithdifferentsoftwaresandannotationsystem.However,thefunctionsofpredictedgeneshavetobefurtheridentifiedbybiologicalanalysis.Oneofthemostpowerfulmethodsassigningfunctiontogeneisthroughinsertionmutagenesiswithtrans-posableelementasDNAsequencetag.Ac/Dstrans-posonsystemhasbecomeaverypopulartoolforgenetaggingandfunctionalgenomicsinvariousplantspecies(Altmannetal.,1995;Chinetal.,1999;Enokietal.,1999;Gre… 相似文献
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在分析我国期刊现状基础上,提出集团化、产业化是我国期刊业发展的必然选择。针对期刊集团化发展中的品牌建设、市场经营等问题,提出自己的看法。 相似文献
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关于提高科技期刊发展力的思考 总被引:2,自引:1,他引:2
阐释了科技期刊发展力的概念和内涵,分析了提高科技期刊发展力的现实意义,探讨了科技期刊发展力的主要构成要素,提出了提高科技期刊发展力的对策思路。 相似文献
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Insertion mutagenesis has become one of the most popular methods for gene functions analysis. Here we report a two-element Ac/Ds transposon system containing enhancer trap and gene trap for gene tagging in rice. The excision of Ds element was examined by PCR amplification. The excision frequency of Ds element varied from 0% to 40% among 20 F2 populations derived from 11 different Ds parents. Southern blot analysis revealed that more than 70% of excised Ds elements reinserted into rice genome and above 70% of the reinserted Ds elements were located at different positions of the chromosome in rice. The result of histochemical GUS analysis indicated that 28% of enhancer trap and 22% of gene trap tagging plants displayed GUS activity in leaves, roots, flowers or seeds. The GUS positive lines will be useful for identifying gene function in rice. 相似文献
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