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目的:探讨不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制。方法:将雄性Wistar大鼠50只随机均分为5组:安静对照组(C)、低负荷运动训练组(LT)、中等负荷运动训练组(MT)、高负荷运动训练组(HT)和极高负荷运动训练组(ST),每组10只。各运动组分别进行6周的跑台运动训练。训练方案结束后,取腓肠肌样本,提取线粒体,测定线粒体柠檬酸合成酶(CS)、异柠檬酸脱氢酶(ICD)和α-酮戊二酸脱氢酶(α-KGDHC)活性;线粒体Ga2+含量、胞浆NADH、NAD+、ATP和ADP含量,以及ICD mRNA转录水平。结果:(1)不同负荷运动训练组线粒体CS、ICD和α-KGDHC的活性均显著高于安静对照组(P < 0.01),且CS和ICD活性由高到低顺序均为:MT组 > HT组 > ST组 > LT组 > C组,α-KGDHC活性由高到低顺序为:HT组 > MT组 > ST组 > LT组 > C组。(2)不同负荷运动训练组线粒体Ca2+ 含量均显著高于安静对照组(P < 0.01),其含量由高到低顺序为:MT组 > HT组 > ST组 > LT组 > C组;胞浆NADH/NAD+和ATP/ADP的比值均显著低于安静对照组(P < 0.01),其比值由低到高顺序为:MT组 < ST组 < HT组 < LT组 < C组。(3)不同负荷运动训练组ICD mRNA转录水平均高于安静对照组(P < 0.01),其水平由高到低顺序为: MT组 > HT组 > ST组 > LT组 > C组。结论:低负荷、中等负荷、高负荷及极高负荷运动训练均可提高大鼠骨骼肌线粒体三羧酸循环功能,且中等负荷运动训练效果最佳。其机制与胞浆NADH/NAD+和ATP/ADP比值、线粒体摄钙能力及限速酶基因的表达有关。 相似文献
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互联网政务服务平台建设是政府职能转变的重要途径,这一新生事物给档案工作带来一定影响。关注已有变化并思考如何实现档案部门与平台的对接是新环境下档案工作的重要内容。本文首先对事由原则与来源原则的发展及演变动因进行了梳理;其次以政府转型的“放管服”需求为框架,分析了政务服务的变革及其对档案工作的影响;最后对事由观在互联网政务服务档案管理中的运用进行了剖析,并深刻反思了档案的来源问题。指出档案部门应当积极应对前端业务活动方式的变化,事由是互联网政务服务平台的突出特征,文件归档和档案管理应当围绕事由展开。 相似文献
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Fadhaa A. Ghafil Bassim I. Mohammad Hussain S. Al-Janabi Najah R. Hadi Hayder A. Al-Aubaidy 《Indian journal of clinical biochemistry : IJCB》2021,36(1):81
Genetic variation in the angiotensin II type 1 receptor (AT1R) has an important effect on the outcome of acute coronary syndrome (ACS) initiated treatment with captopril. This study aims to investigate the impact of genetic polymorphism of AT1R (rs5186 and rs275651) on the ACS outcome in Iraqi patients treated with captopril. A total of 250 Iraqi individuals with ACS were included in this case—control study and they were divided into two study groups; Study group 1 included 125 participants who were prescribed captopril, 25 mg twice daily and study group 2 included 125 participants who received no captopril as part of their ACS treatment (control study). The AT1R gene (rs5186) CC genotype was found to be associated with ST-elevation myocardial infarction (STEMI) (Odd’s ratio (O.R) = 1.2, P = 0.7), while AC was associated with Non-ST-elevation myocardial infarction (NSTEMI) and unstable angina (UA) (O.R = 1.2, P = 0.8). AC genotype is more prone to have Percutaneous coronary intervention (PCI) after ACS attack (O.R = 1.2, P = 0.6). CC genotype had a risk to get less improvement (O.R = 1.6, P = 0.5), so might require higher doses of captopril during acute coronary insult. The AT1R gene (rs275651) AA genotype was associated with UA (O.R = 1.3, P = 0.9). AA and AT genotypes were more prone to have PCI after ACS attack (O.R = 3.9 P = 0.2, O.R = 3.5, P = 0.3 respectively) and thus requiring higher doses of captopril. We conclude that the AT1R rs5186, rs275651 genetic polymorphisms might partially affect the clinical outcome of ACS patients treated with captopril and might have captopril resistance which requires higher doses. 相似文献
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Mirjam A. Twigt 《Popular Communication》2019,17(2):171-184
ABSTRACTFor many forcefully displaced people worldwide, notions around one’s sense of “home” and “place” in the world are perpetually unsettled. This article explores how digital technologies interact with embodied, material experiences within the geographical location refugees are residing in. Empirical examples from ethnographic research conducted among Iraqi urban refugees, living in prolonged uncertainty in Jordan, shows how situated experiences of legal, material, and social uncertainty reinforce particular mediated socialities. Pivotal studies have shown how digital connections engender virtual home-making practices and a wide variety of connected presences. This study points to the other side of the same coin: the cultivation of “absent presence.” “Absent presence” refers to feelings—the ambivalence that experiences of prolonged displacement bring about—but also to active disengagement and affective tactics in response. These engender a further separation from the physical world where forced migrants are not deemed welcome. 相似文献
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A. Miller 《Journal of Web Librarianship》2019,13(2):127-155
The emerging creative practices of digital collections are increasing in prominence thanks to the access to, and appeal of, data visualizations. Interactive data visualizations, such as a map, timeline, chart, graph, or artist’s rendering of data, enable users to pursue their own research interests within a collection and participate in new areas of research. Use of these data visualizations enhances the scope of the collection and enables a modern and diverse thematic collection to emerge. This article considers the question: Does the addition of data visualizations affect how users participate with a research collection? Impacts on collection development, application of user-centered design, and analysis of users’ exploratory strategies are discussed. Examples of data visualizations used in digital humanities projects and digital collections are presented as case studies. 相似文献
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Children are sensitive to a number of considerations influencing distributions of resources, including equality, equity, and reciprocity. We tested whether children use a specific type of reciprocity norm—market norms—in which resources are distributed differentially based strictly on amount offered in return. In two studies, 195 children 5–10 years and 60 adults distributed stickers to friends offering same or different amounts of money. Overall, participants distributed more equally when offers were the same and more unequally when offers were different. Although sensitive to why friends offered different amounts of money, children increasingly incorporated market norms into their distributions with age, as the oldest children and adults distributed more to those offering more, irrespective of the reasons provided. 相似文献
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针对多旋翼无人机目标的识别问题,提出一种基于伽柏(Gabor)变换的瞬时频率估计与快速傅里叶变换(FFT)相结合的微多普勒特征提取算法。首先建立多旋翼无人机旋翼回波模型,并通过仿真分析叶片数目、旋翼转速和初始相位等参数对微多普勒特征的影响,利用Gabor变换得到时频特征。在此基础上通过瞬时频率极大值法提取微多普勒频率,并对瞬时频率采用FFT提取旋翼数和转动频率,从而获得叶片长度估计值。实测数据验证了该算法较为准确地提取无人机的微多普勒参数。 相似文献
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[目的/意义] 针对包含单一类型知识单元的知识网络难以全面反映学科知识结构的问题,提出一种从多维度进行知识网络结构融合的方法,为学科领域知识结构挖掘提供借鉴。[方法/过程] 利用LDA及TF-IDF方法抽取学科知识单元,然后运用语义相似度和关键词共现分析方法构建3个学科知识子网络:主题网络、关键词网络和实体网络,并采用空间节点传递对齐方法对齐子网络节点,接着设计基于图卷积操作的自编码模型对知识节点进行表示,最后通过计算余弦相似度重构学科知识网络。[结果/结论] 实验部分以人工智能领域为例,构建融合主题、关键词和实体的学科知识网络并展开分析,实验结果表明,本文所提方法能有效地揭示学科领域研究内容和知识结构,为学科知识发现与组织研究提供有益参考。 相似文献