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Jyoti P. Gurung Moein Navvab Kashani Sanaz Agarwal Gonzalo Peralta Murat Gel Matthew A. B. Baker 《Biomicrofluidics》2021,15(3)
Many motile bacteria are propelled by the rotation of flagellar filaments. This rotation is driven by a membrane protein known as the stator-complex, which drives the rotor of the bacterial flagellar motor. Torque generation is powered in most cases by proton transit through membrane protein complexes known as stators, with the next most common ionic power source being sodium. Sodium-powered stators can be studied through the use of synthetic chimeric stators that combine parts of sodium- and proton-powered stator proteins. The most well studied example is the use of the sodium-powered PomA-PotB chimeric stator unit in the naturally proton-powered Escherichia coli. Here we designed a fluidics system at low cost for rapid prototyping to separate motile and non-motile populations of bacteria while varying the ionic composition of the media and thus the sodium-motive force available to drive this chimeric flagellar motor. We measured separation efficiencies at varying ionic concentrations and confirmed using fluorescence that our device delivered eightfold enrichment of the motile proportion of a mixed population. Furthermore, our results showed that we could select bacteria from reservoirs where sodium was not initially present. Overall, this technique can be used to implement the selection of highly motile fractions from mixed liquid cultures, with applications in directed evolution to investigate the adaptation of motility in bacterial ecosystems. 相似文献
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Cultural acceptability and personal willingness of Iranian students toward cadaveric donation 
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下载免费PDF全文 Jamal Abbasi Asl Hossein Nikzad Aliakbar Taherian Mohammad Ali Atlasi Homayoun Naderian Gholamabbas Mousavi Milad Motalebi Kashani Abdollah Omidi 《Anatomical sciences education》2017,10(2):120-126
Cadaver dissection stands as a crucial component in medical curricula around the world, although computer‐based multimedia programs have been introduced in order to replace the need for cadaver donations. Due to a decrease in the number of unclaimed bodies and rather few donations, there is an insufficient number of cadavers for anatomical studies in Iran. This study was carried out to evaluate medical students' awareness and willingness regarding body donation in Kashan University of Medical Sciences, Iran. In this study, a questionnaire was designed to focus on the cultural acceptability and personal willingness to donate one's body after death. Students from the university's anatomy classes (n = 331) participated in this study. Seventy‐seven percent of the students expressed their agreement toward the idea of utilizing body donation services, though only 25.4% of participants were willing to donate their own bodies. None of the demographic factors were associated with cultural acceptability or personal willingness towards body donation. These findings indicated that besides “payment”, other factors were associated with students' willingness to become donors. All factors of awareness except “previous awareness of organization” were associated with cultural acceptability. In this study, students suggested that encouraging people to register for body donation using mass media (25.6%) and teaching students to respect cadavers in the dissection environment (24.8%) were the best solutions for addressing the lack of cadavers. These findings indicated that a lack of awareness about body donation might be the main factor responsible for unwillingness towards body donation; therefore, improving the public's awareness and addressing the willingness of students regarding body donation may help overcome the current lack of donated cadavers. Anat Sci Educ 10: 120–126. © 2016 American Association of Anatomists. 相似文献
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The role of information therapy in reducing anxiety in patients undergoing in vitro fertilisation treatment 下载免费PDF全文
下载免费PDF全文 Sara Ahmadizadeh Ashraf Sadat Bozorgi Ladan Kashani 《Health information and libraries journal》2017,34(1):86-91
This article is the first in the feature to come from Iran. The lead author, Sara Ahmadizadeh, manages the Library at Arash Women's Hospital and was awarded an MA in Information Science and Knowledge and Librarianship from Tehran Azad University in July 2015. Her MA dissertation was innovative and ambitious in that it looked at the impact of information therapy on levels of anxiety amongst women undergoing IVF treatment. Her study demonstrated that quality information sessions, delivered in a structured way, can have a positive impact on patients’ awareness of their condition and can reduce anxiety. A. M. 相似文献
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