Thyroid hormones status during pregnancy in normal Iranian women     

Nosratollah Zarghami  Mohammad Rohbani-Noubar  Ali Khosrowbeygi 《Indian journal of clinical biochemistry : IJCB》2005,20(2):182-185

Pregnancy is associated with significant, but reversible changes in thyroid function studies, which are among the most profound seen as a result of a normal physiologic state. The present study was carried out to find out alterations in thyroid function tests in each trimester in normal pregnant women as compared to non-pregnant women in Tabriz-Iran. A case-control study designed with 229 normal pregnant women that randomly selected from the first (64 samples), the second (92 samples), and the third (73 samples) trimesters and 250 randomly selected non-pregnant healthy female controls. Age range in both groups was 16–40 years. Thyroid function tests carried out by measuring serum levels of thyroid stimulating hormone (TSH), free and total thyroxin (FT₄, TT₄), and free and total triiodothyronine (FT₃, TT₃) by commercially available radio immunoassay kits. We found that mean TT₄ increased progressively during pregnancy. Our study showed increasing in serum levels of TT₃ in the second trimester and then declining during the third trimester compared with non-pregnant women. We showed that FT₄ strongly decreased during the third trimester. Free T₃ showed declining in the second and third trimesters. Mean TSH did not show significant difference in each trimester compared with non-pregnant women. The thyroid function tests in pregnancy should be interpreted against gestational age-related reference intervals to avoid mis-interpretation of thyroid function during pregnancy.  相似文献   

Seminal plasma levels of free 8-isoprostane and its relationship with sperm quality parameters     

Ali Khosrowbeygi  Nosratollah Zarghami 《Indian journal of clinical biochemistry : IJCB》2008,23(1):49-52

There is growing evidence that damage to spermatozoa by reactive oxygen species play a key role in male infertility. The aim of this study was to assess seminal plasma free 8-Isoprostane levels in men with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia compared to normozoospermic males and its correlation with seminal parameters. The case group consisted of men with asthenozoospermia (n=15), asthenoteratozoospermia (n=16) and oligoasthenoteratozoospermia (n=15). The control group consisted of 16 males with normozoospermia. After Purification of Free 8-Isoprostane by affinity column, its concentration was measured by enzyme immunoassay method. Free 8-Isoprostane evaluation showed significantly greater values in the total case group (n=46) versus control group (18.23±3.56 vs 2.6±0.38 ng/ml). In each case group free 8-Isoprostane also showed a significant increasing compared to normozoospermic males. Free 8-Isoprostane showed an inversely significant correlation with sperm motility and sperm morphology. Lipid peroxidation could have significant role in etiology of sperm abnormalities. Measurement of 8-Isoprostane can be used as a specific biomarker for assessing lipid peoxidation in sperm.  相似文献   

Seminal plasma levels of 15-F2α-isoprostane, malondialdehyde and total homocysteine in normozoospermic and asthenozoospermic males     

Nosratollah Zarghami  Ali Khosrowbeygi 《Indian journal of clinical biochemistry : IJCB》2005,20(2):86-91

It has been proposed that oxidative stress plays an important role in male infertility. The aims of this study were to compare seminal plasma levels of 15-F2t-isoprostane (8-iso-PGF2α), malondialdehyde (MDA), and total (sum of free and bound) homocysteine (tHcy) from normozoospermic vs. asthenozoospermic men, and to examine the relationships between tHcy and lipid peroxidation products. The study was a case-control study with a simple random sampling. The case group was consisted of 15 asthenozoospermic males. This group was compared with 15 normozoospermic men. Seminal plasma levels of 15-F2α-isoprostane and tHcy were measured using commercially available enzyme immunoassay (EIA) kits. MDA levels were determined by the thiobarbituric acid (TBA) assay. The Mann-Whitney U test was used to compare two groups. Coefficients of correlation were calculated using Spearman’s correlation analysis. All hypothesis tests were two-tailed with statistical significance assessed at the p value <0.05 level. MDA levels were higher in asthenozoospermic subjects than in control subjects (0.72±0.06 μM vs. 0.40±0.06 μM; p<0.05). No differences were seen in 15-F2α-isoprostane levels in asthenozoospermic subjects and controls (65.00±3.20 pg/ml vs. 58.17±4.12 pg/ml; p>0.05). Interestingly, tHcy levels were to be slightly higher in asthenozoospermic subjects than in controls (6.18±1.17 μM vs. 4.8±0.52 μM). Sperm motility was inversely correlated with seminal plasma 15-F2α-isoprostane and MDA levels, respectively (p<0.05). In summary, seminal plasma levels of 15-F2α-isoprostane and tHcy showed no significant difference between normozoospermic and asthenozoospermic men. Sperm motility was not correlated with seminal plasma levels of tHcy. No relationship was found between tHcy and lipid peroxidation.  相似文献