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In this work, we introduce a method for the soft-lithography-based fabrication of rigid microstructures and a new, simple bonding technique for use as a continuous-flow cell lysis device. While on-chip cell lysis techniques have been reported previously, these techniques generally require a long on-chip residence time, and thus cannot be performed in a rapid, continuous-flow manner. Microstructured microfluidic devices can perform mechanical lysis of cells, enabling continuous-flow lysis; however, rigid silicon-based devices require complex and expensive fabrication of each device, while polydimethylsiloxane (PMDS), the most common material used for soft lithography fabrication, is not rigid and expands under the pressures required, resulting in poor lysis performance. Here, we demonstrate the fabrication of microfluidic microstructures from off-stoichiometry thiol-ene (OSTE) polymer using soft-lithography replica molding combined with a post-assembly cure for easy bonding. With finite element simulations, we show that the rigid microstructures generate an energy dissipation rate of nearly 107, which is sufficient for continuous-flow cell lysis. Correspondingly, with the OSTE device we achieve lysis of highly deformable MDA-MB-231 breast cancer cells at a rate of 85%, while a comparable PDMS device leads to a lysis rate of only 40%. 相似文献
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On 29 September 2016, the Indian army conducted a surgical strike along the India–Pakistan border. The mainstream news media in India followed the event with assertive nationalistic rhetoric. What was supposed to be a covert military operation against terrorism became morphed into political rhetoric aggravated by the unwarranted jingoism of television news channels and social media. The coverage of the strike on television news is typically characterized by a confluence of militant nationalist discourses, and the ideologically imbued labelling of specific communities. Within this context, drawing from the close reading of the coverage, this article analyses how Indian television news sustains the construction of a fictive “we”, conflated with the government policies and military strategies, and speaks for a supposedly homogeneous national consensus that also consciously obscures the dissent through minority voices. The article emphasizes the relationship between communities, formal politics, and the supposedly non-political spaces and practices of news media in India. 相似文献
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Frame loss error concealment for SVC 总被引：2，自引：0，他引：2
INTRODUCTION Real-time transmission of video data in network environments, such as wireless and Internet, is a challenging task, as it requires coding efficiency, network friendliness and error robustness. Scalability is a possible solution for network adaptive applica- tions. The Scalable Extension of H.264/AVC (SVC) (JVT of ISO/IEC MPEG & ITU-T VCEG, 2005b) aims at achieving both high compression performance and adaptivity for video delivery over heterogeneous networks. SVC… 相似文献
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Pakrashi Anita Pandit Srikanta Bandyopadhyay Sandip K. Pakrashi Satyesh C. 《Indian journal of clinical biochemistry : IJCB》2003,18(1):15-21
Post-treatment of the indomethacin induced ulcerated rats at the optimal dose of 100 mg/kg body-wt. orally for 7 consecutive
days with the lyophilized aqueous extract of the fruits ofPhyllanthus emblica L. syn.Emblica officinalis Gaertn. (Euphorbiaceae) exhibited highly significant (p<0.001) enhancement of secretion of catalase, reduced glutathione
and decrease in malonyldialdehyde (MDA). Furthermore, the gross morphological observation and highly significant (p<0.001)
decrease of ulcer index (81.43%) indicated healing effect of the extract on gastric ulcer. 相似文献
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