排序方式： 共有19条查询结果，搜索用时 15 毫秒
1.
Sungmin Hong Pei-Hsiang Tsou Chao-Kai Chou Hirohito Yamaguchi Chin B. Su Mien-Chie Hung Jun Kameoka 《Biomicrofluidics》2012,6(2)
A simple microfluidic 3D hydrodynamic flow focusing device has been developed and demonstrated quantitative determinations of quantum dot 525 with antibody (QD525-antibody) and hemagglutinin epitope tagged MAX (HA-MAX) protein concentrations. This device had a step depth cross junction structure at a hydrodynamic flow focusing point at which the analyte stream was flowed into a main detection channel and pinched not only horizontally but also vertically by two sheath streams. As a result, a triangular cross-sectional flow profile of the analyte stream was formed and the laser was focused on the top of the triangular shaped analyte stream. Since the detection volume was smaller than the radius of laser spot, a photon burst histogram showed Gaussian distribution, which was necessary for the quantitative analysis of protein concentration. By using this approach, a linear concentration curve of QD525-antibody down to 10 pM was demonstrated. In addition, the concentration of HA-MAX protein in HEK293 cell lysate was determined as 0.283 ± 0.015 nM. This approach requires for only 1 min determining protein concentration. As the best of our knowledge, this is the first time to determinate protein concentration by using single molecule detection techniques. 相似文献
2.
Higher education reform in perspective: The Japanese experience 总被引：1，自引：0，他引：1
Akihiro Itoh 《Higher Education》2002,43(1):7-25
Japanese colleges and universitiesare in an age of great transition. Currentreforms can be counted as some of the mostextensive and radical changes in the history ofJapanese higher education. Although the agendaof recent reforms has been conditioned bycontemporary socio-political circumstances,many of the ongoing changes are rooted inhistory. In this article, the reforms ofJapanese higher education are examined from along-range historical perspective. Thoughpre-war years are briefly mentioned, the mainconcern here is with reforms after World WarII. 相似文献
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A progenitor article for this work can be found in Public Library Quarterly 27, no. 4. This study is the first to report on the types of contents in children's web pages and the characteristics of Web-OPACs for children in public library websites in Japan. This study reveals that children's web pages, in general, place more emphasis on providing guidance on library services and on the use of information resources for reference services, rather than on helping children with regular learning or providing research assistance to adults on children's services. Other findings are reported as well. 相似文献
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Akihiro Ogawa 《International Journal of Lifelong Education》2013,32(5):601-614
The concept of ‘lifelong learning’ or shōgai gakushū has rapidly become one of the topmost priorities in Japan’s education policy agenda. This was considerably evident in December 2006 when the term ‘lifelong learning’ was added to Japan’s educational charter, the Fundamental Law of Education. This paper explores, as a means to develop Japan’s new lifelong learning policy, the lessons that can be learnt through an examination of the European countries’ efforts to build a knowledge economy, where lifelong learning is regarded as the key solution in overcoming several important social and economic concerns. In this paper, I first examine the current situation of lifelong learning in Japan, employing the ethnographic data that I have collected since 2001. Second, I provide a brief review of the European lifelong learning policy, which is one of the priority guidelines in the European Union. Under the Lisbon Strategy, for example, the argument on European lifelong learning theoretically centres on developing human capital in order to survive in the global knowledge economy. Lastly, referring to the European experience over the past decade, I propose to directly connect Japan’s latest policy development regarding lifelong learning with the trend of building human capital through lifelong learning in order to enhance its competitiveness in the era of globalisation. 相似文献
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This paper presents a data envelopment analysis (DEA)/Malmquist index methodology for measuring the change in R&D efficiency at both firm and industry levels. Letting each of ten firms in each year be a separate decision-making unit, and employing one input and three outputs in a DEA case of R&D activity input-output lag, we measure “total factor R&D efficiency” change of Japanese pharmaceutical firms for decade 1983-1992 as defined by the period of R&D input. Decomposing Malmquist index into catch-up and frontier shift components and using “cumulative indices” proposed in this study, we evaluate R&D efficiency change for each firm and empirically show that R&D efficiency of Japanese pharmaceutical industry has almost monotonically gotten worse throughout the study decade. 相似文献
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Ikushi Yamaguchi 《Int J Intercult Relat》2009,33(1):21-31
This study looked into possible relationships between workplace organizational communication tactics, workers’ feelings of trust, and workers’ perceptions of procedural justice. Raw data was collected by questionnaires, and structural equation modeling (SEM) was used to reveal the relationships. The subjects in the study were Japanese (N = 417) and American (N = 591) workers, surveyed in a period running up to late May 2004. For both the Japanese and the Americans, perceived procedural justice was influenced positively by rational communication tactics, and negatively by hard communication tactics; and soft communication tactics did not relate to perceived procedural justice. In the case of Japanese workers, it was found that, after controlling for perceived procedural justice, soft, rational, and hard communication tactics affected trust in different ways: positively, positively, and negatively, respectively. Therefore, perceived procedural justice had a partially mediating effect on the relationships between rational and hard communication tactics and trust. In contrast, for the Americans, there were no direct relationships between communication tactics and trust; rather, there were indirect effects from rational and hard communication tactics, via perceived procedural justice as a full mediator. The influence of three communication tactics on trust was much stronger for Japanese workers than their American counterparts. On the other hand, the influence of hard and rational communication tactics on perceived procedural justice and that of perceived procedural justice on trust were much stronger for American workers than their Japanese counterparts. Based on the results, some academic and practical implications are presented. 相似文献
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日本国立大学法人化改革前,拥有附属医院的国立大学通过向财政融资资金借款来筹措附属医院发展过程中所需的部分经费。国立大学法人化改革后,日本政府成立了国立大学财务经营中心,负责对国立大学借贷资金的筹措、贷与及管理。向该中心借款且金额较大的大学主要是旧帝国大学、有附属医院的综合大学及医科大学这三类国立大学。日本国立大学的组织规模和声威与其获得借贷的能力成正相关。在国立大学法人化改革的第一个六年"中期阶段计划"期间,日本国立大学借贷融资主要是通过国立大学财务经营中心来获得的,但也出现了向金融机构进行长期贷款的借贷方式。 相似文献
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Leble V Lima R Dias R Fernandes C Ishikawa T Imai Y Yamaguchi T 《Biomicrofluidics》2011,5(4):44120-4412015
In microcirculation, red blood cells (RBCs) flowing through bifurcations may deform considerably due to combination of different phenomena that happen at the micro-scale level, such as: attraction effect, high shear, and extensional stress, all of which may influence the rheological properties and flow behavior of blood. Thus, it is important to investigate in detail the behavior of blood flow occurring at both bifurcations and confluences. In the present paper, by using a micro-PTV system, we investigated the variations of velocity profiles of two working fluids flowing through diverging and converging bifurcations, human red blood cells suspended in dextran 40 with about 14% of hematocrit level (14 Hct) and pure water seeded with fluorescent trace particles. All the measurements were performed in the center plane of rectangular microchannels using a constant flow rate of about 3.0 × 10−12 m3/s. Moreover, the experimental data was compared with numerical results obtained for Newtonian incompressible fluid. The behavior of RBCs was asymmetric at the divergent and convergent side of the geometry, whereas the velocities of tracer particles suspended in pure water were symmetric and well described by numerical simulation. The formation of a red cell-depleted zone immediately downstream of the apex of the converging bifurcation was observed and its effect on velocity profiles of RBCs flow has been investigated. Conversely, a cell-depleted region was not formed around the apex of the diverging bifurcation and as a result the adhesion of RBCs to the wall surface was enhanced in this region. 相似文献
10.
Yumiko Otsuka Yukuo Konishi So Kanazawa Masami K. Yamaguchi Hervé Abdi Alice J. O'Toole 《Child development》2009,80(4):1259-1271
This study compared 3- to 4-month-olds' recognition of previously unfamiliar faces learned in a moving or a static condition. Infants in the moving condition showed successful recognition with only 30 s familiarization, even when different images of a face were used in the familiarization and test phase (Experiment 1). In contrast, infants in the static condition showed successful recognition only when the familiarization duration was lengthened to 90 s and when the same image was used between the familiarization and test phase (Experiments 2 and 3). Furthermore, presentation of multiple static images of a face did not yield the same level of performance as the moving condition (Experiment 4). These results suggest that facial motion promotes young infants' recognition of unfamiliar faces. 相似文献