Cloning and efficient expression of <Emphasis Type="Italic">Bacillus sp.</Emphasis> BH072 <Emphasis Type="Italic">tasA</Emphasis> gene in <Emphasis Type="Italic">Escherichia coli</Emphasis>     

韩烨  樊洁  周志江  檀茜倩  赵鑫 《天津大学学报(英文版)》2015,21(1):26-31

The Bacillus strain BH072 isolated from a honey sample showed strong antifungal activity against phytopathogen. Gene cloning test demonstrated that the strain had a tasA gene encoding an antifungal TasA protein. Although the wild strain simultaneously produced various antifungal substances, only the physicochemical property and antifungal activity of TasA protein were unclear due to the difficulty in extraction. In this study, tasA gene encoding the protein from Bacillus sp. BH072 was amplified by using the polymerase chain reaction (PCR) method and cloned into pET 28a (+) vector, and then expressed in host cells Escherichia coli BL21 (DE3). The expressed proteins were collected by centrifugation and ultrasonic treatment, and then purified by using nickel-nitrilotriacetic acid (Ni-NTA) metal affinity column and dialysis methods. The result of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) test showed that an expected protein band appeared with a size of 31 kDa. The expressed products possessed antifungal activity against the phytopathogenic indicator strain Botrytis cinerea. A genetically engineered strain tasA of E. coli was established in this study which can efficiently express Tas A protein.  相似文献   

完善实验室管理模式实现资源共享   总被引：4，自引：0，他引：4  

张丽霞  周志江 《中国轻工教育》2006,(3):50-51

实验教学作为高等教育体系中的一个教学环节,在整个教学过程中具有极其重要的作用。然而,由于过去的实验室管理体制存在着这样或那样的弊端,严重影响了实验室的建设与发展,主要表现在:专业分得过细过窄,一个实验室仅为一两门课程服务;实验室规模小,功能单一,人员、设备和房屋的  相似文献