Characteristics of photosynthesis in rice plants transformed with an antisense Rubisco activase gene     

金松恒  蒋德安  李雪芹  孙骏威 《Journal of Zhejiang University. Science. B》2004,(8)

INTRODUCTION Ribulose-1,5-bisphosphate carboxylase/oxy-genase (Rubisco) is a key enzyme that initiates bothphotosynthetic and photorespiratory carbon me-tabolism. However, Rubisco has to be activated andcarbamylated to become catalytically competent.Rubisco activase (RCA) can alter the activity ofRubisco by facilitating the dissociation of tightlybound sugar phosphates from Rubisco in a processthat requires ATP hydrolysis. RCA, a chloroplastprotein encoded by a nuclea…  相似文献   

通过果蝇杂交实验培养学生的科研意识和能力   总被引：2，自引：0，他引：2  

李素芳  孙骏威  朱银焕  李丹青 《实验技术与管理》2007,24(2):16-18

该文以普通遗传学课程中的果蝇杂交综合实验为载体,培养学生从事生物科学研究的意识和基本技能。安排学生自主设计杂交组合,并在课下完成了从亲本杂交至F2代出现等系列实验过程,引导学生摸拟完成了科研活动中的实验设计、实施、数据分析和论文写作的系统过程,为学生大四的毕业设计以及今后从事科研工作奠定基础。  相似文献   

生物学野外实习中安全工作的保障     

黄丽红  孙骏威  林芳  余晓霞  徐爱春 《教育教学论坛》2013,(27):123-124

生物学野外实习是生物学实践教学的重要环节,其是一个系列工程,涉及到很多方面,但安全始终排第一位。本文总结了本单位野外实习的经验,简要地分析了野外实习中可能遇到的安全隐患,并给出了相应的安全对策。  相似文献   

加强植物学教学改革的基础建设研究     

王飞娟  朱诚  蔡冲  孙骏威  郝培应 《出国与就业》2011,(17)

植物学是高等院校生物相关专业本科生的一门主干必修专业基础课,针对增强植物学理论与实践教学效果,介绍了其教学改革中的一些措施,包括培养学生对本专业热度、引导学生对专业的正确认识及教材更新等多种手段,以期提高植物学课程的教学效果。  相似文献   

生物学野外实习考核评价体系的探索和改革     

孙骏威  黄丽红  林芳  余晓霞  徐爱春 《教育教学论坛》2013,(51):26-27

生物学野外实习是培养生物学专业人才综合实践能力的关键环节之一。通过对其他高校的调研,制定我校的生物学野外实习课程考核评价体系。在这考核评价体系中,学生的总成绩由小组成绩和个人成绩组成,而小组成绩和个人成绩又各由5小项组成。制定的考核评价体系得到学生的普遍认同,可很好地反映出学生的真实水平。  相似文献   

Characteristics of photosynthesis in rice plants transformed with an antisense Rubisco activase gene     

金松恒  蒋德安  李雪芹  孙骏威 《浙江大学学报(A卷英文版)》2004,5(8):897-899

Transgenic rice plants with an antisense gene inserted via Agrobacterium tumefaciens were used to explore the impact of the reduction of Rubisco activase (RCA) on Rubisco and photosynthesis. In this study, transformants containing 15% to 35% wild type Rubisco activase were selected, which could survive in ambient CO2 concentration but grew slowly compared with wild type controls. Gas exchange measurements indicated that the rate of photosynthesis decreased significantly, while stomatal conductance and transpiration rate did not change; and that the intercellular CO2 concentration even increased. Rubisco determination showed that these plants had approximately twice as much Rubisco as the wild types,although they showed 70% lower rate of photosynthesis, which was likely an acclimation response to the reduction inRubsico activase and/or the reduction in carbamylation.  相似文献