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Salmanzadeh A Kittur H Sano MB C Roberts P Schmelz EM Davalos RV 《Biomicrofluidics》2012,6(2):24104-2410413
Ovarian cancer is the leading cause of death from gynecological malignancies in women. The primary challenge is the detection of the cancer at an early stage, since this drastically increases the survival rate. In this study we investigated the dielectrophoretic responses of progressive stages of mouse ovarian surface epithelial (MOSE) cells, as well as mouse fibroblast and macrophage cell lines, utilizing contactless dielectrophoresis (cDEP). cDEP is a relatively new cell manipulation technique that has addressed some of the challenges of conventional dielectrophoretic methods. To evaluate our microfluidic device performance, we computationally studied the effects of altering various geometrical parameters, such as the size and arrangement of insulating structures, on dielectrophoretic and drag forces. We found that the trapping voltage of MOSE cells increases as the cells progress from a non-tumorigenic, benign cell to a tumorigenic, malignant phenotype. Additionally, all MOSE cells display unique behavior compared to fibroblasts and macrophages, representing normal and inflammatory cells found in the peritoneal fluid. Based on these findings, we predict that cDEP can be utilized for isolation of ovarian cancer cells from peritoneal fluid as an early cancer detection tool. 相似文献
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Shalvika Gupta Vijetha Shenoy Belle Ramya Kumbarakeri Rajashekhar Sushma Jogi RV Krishnananda Prabhu 《Indian journal of clinical biochemistry : IJCB》2018,33(4):445-449
Cholinesterases belongs to class hydrolases. There are two types acetylcholinesterase and butyryl cholinesterase. Acetylcholinesterase present in nerve endings and also in the RBC membrane. It helps to maintain the shape and size of RBCs. Any change in shape and size of RBCs may affect the activity of Acetylcholinesterase. Thus this study aimed to estimate RBCs Acetylcholinesterase enzyme activity in various types of anemias and correlate the RBCs Acetylcholinesterase enzyme activity with various hematological indices such as Erythrocyte Sedimentation Rate (ESR), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), Mean Corpuscular Volume (MCV), Red cell Distribution Width (RDW) etc. After obtaining ethical approval from Institutional ethics committee total of 100 samples were collected from Clinical Biochemistry laboratory, Kasturba Medical College, Manipal, Manipal University. 25 were having normal RBC indices, 12 with hemolytic anemia, 26 with microcytic anemia and 26 with macrocytic anemia based on peripheral smear report and RBC indices. Acetylcholinesterase were measured using Ellman’s method. RBC acetylcholinesterase activity was significantly increased in microcytic anemia (58.13 ± 5.4) and macrocytic anemia (76.87 ± 6.7) than normal group (37.62 ± 2.71). Also increased RBC acetylcholinesterase was seen in hemolytic anemia (48.11 ± 5.18) but the increase is not statistically significant. RBC acetylcholinesterase correlated negatively with hemoglobin (r = ?0.356, p = 0.001) and positively with RDW (r = 0.31, p = 0.003). To conclude RBC acetylcholinesterase activity can be used as one of the potential marker for various types of anemia. 相似文献
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Several factors have increased teachers’ frustrations in the regular classroom as they try to meet the needs of students with varying levels of ability. Limited educational funding, the attitude that gifted students can do without special services, and a push for increased heterogeneous grouping have all contributed to the teacher's problem. The Mustard Seed Project was developed and supported by a grant from the Javits Gifted and Talented Students Education program to train teachers to differentiate for student needs by curricular adaptations. The training for teachers modeled the process of individualization for each learner's level, pace and style. A follow‐up support system of human and material resources was implemented to sustain the teachers through the difficult transition required of change. The sites participating in this study were all rural, ethnically diverse, and economically disadvantaged. Profiles of six gifted fifth‐graders were developed using interviews and classroom observations. The research questions sought to determine the extent of the gifted child's intellectual challenge, exploration of in‐depth selected topics of interest, and social and emotional satisfaction in adapted classrooms. 相似文献
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