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We present a microfluidic approach to characterizing temperature-dependent biomolecular interactions. Solvated L-arginine vasopressin (AVP) and its immobilized RNA aptamer (spiegelmer) were allowed to achieve equilibrium binding in a microchip at a series of selected temperatures. Unbound AVP were collected and analyzed with matrix-assisted laser desorption∕ionization mass spectrometry (MALDI-MS), yielding melting curves that reveal highly temperature-dependent zones in which affinity binding (36–45 °C) or dissociation (25–33 °C and 50–65 °C) occurs. Additionally, temperature-dependent binding isotherms were constructed; from these, thermodynamic quantities involved in binding were extracted. The results illustrated a strong change in heat capacity of interaction for this system, suggesting a considerable thermodynamic influence controlling vasopressin-spiegelmer interaction. 相似文献
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Sreten B. Stojanovic Dragutin Lj. Debeljkovic Dragan S. Antic 《Journal of The Franklin Institute》2014
In this paper, the problem of finite-time stability analysis for linear discrete time-delay systems is studied. By using the classical Lyapunov-like functional and Lyapunov-like functionals with power or exponential functions, some sufficient conditions for finite-time stability of such systems are proposed in the form of the linear matrix inequalities. The six aggregate norm approximations of the delayed states are introduced to establish the relations between the classical Lyapunov-like functional and its difference. To further reduce the conservatism of stability criteria, three inequalities with delayed states for the estimation of Lyapunov-like functional are proposed. A numerical example is included to illustrate the effectiveness and advantage of the proposed methods. 相似文献
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Sreten B. Stojanovic 《Journal of The Franklin Institute》2017,354(11):4549-4572
The problem of finite-time stability (FTS) for discrete-time systems with interval time-varying delay, nonlinear perturbations and parameter uncertainties is considered in this paper. In order to obtain less conservative stability criteria, a finite sum inequality with delayed states is proposed. Some sufficient conditions of FTS are derived in the form of the linear matrix inequalities (LMIs) by using Lyapunov–Krasovskii-like functional (LKLF) with power function and single/double summation terms. More precisely estimations of the upper bound of the initial value of LKLF and the lower bound of LKLF are proposed. As special cases, the FTS of nominal discrete-time systems with constant or time-varying delay is considered. The numerical examples are presented to illustrate the effectiveness of the results and their improvement over the existing literature. 相似文献
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Gordana Bjelakovic Petar Miladinovic Tatjana Jevtovic-Stoimenov Ivana Stojanovic Jelenka Nikolic Dusica Pavlovic Gordana Kocic Marija Bjelakovic Mira Ilic Dusan Sokolovic Jelena Basic 《Indian journal of clinical biochemistry : IJCB》2015,30(1):84-88
Arginase activity is important in polyamines and nitric oxide production which are required for the normal growth of placenta and embryo. A considerable arginase activity is observed in amniotic fluid in women at the end of pregnancy. Lecithin to sphingomyelin (L/S) ratio is widely used in order to assess fetal lung immaturity and prevention of neonatal respiratory distress syndrome, the major cause of neonatal morbidity and mortality. The purpose of our study was to determine if there is a relationship between arginase activity and L/S ratio in amniotic fluid. The study included 170 pregnant women, 18–43 years old, with normal and pathological pregnancy. The arginase activity was measured on the basis of the determination of the amount of liberated ornithine from arginine as substrate. The L/S ratio was done by using a thin layer chromatography. Increased level of arginase activity correlates with the fetal lung maturity. Arginase activity and L/S values may be useful biochemical data, for intrauterine baby maturity. 相似文献
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浅论计算机网络用语对现代汉语的影响及其规范 总被引：1，自引：0，他引：1
林纲 《徐州师范大学学报(哲学社会科学版)》2001,27(3):85-87
计算机网络用语是现代汉语新词的来源之一，本文试从三个方面对其进行探讨：1、我国计算机网络用语的现状。2、我国计算机网络用语的影响。3、我国计算机网络用语的规范。 相似文献
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