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Mohammad Abdi Mohammad Said Hakhamaneshi Mohammad Reza Alaei Namam-Ali Azadi Rahim Vakili Daniel Zamanfar Mohammad Taghikhani Shohreh Khatami 《Indian journal of clinical biochemistry : IJCB》2015,30(3):318-322
Methods for assaying lysosomal diseases in dried blood samples are very useful today due to its several advantages related to the stability of samples, its transportation, handled and analysis, and its potential use for newborn screening compared to traditional methods in leucocytes samples. For this reason, it is important to validate these assays before being used in routine laboratory. Because of different in biological markers based on ethnicity, we aimed this study to validation a DBS-based fluorometric assay for measurement of α-l-Iduronidase activity for diagnosis of MPS I patients in Iran. DBS samples were collected from 15 MPS I patients and 60 healthy age matched subjects. Diagnostic value, biological variance and α-l-Iduronidase activity were determined. DBS α-l-Iduronidase activity was significantly higher in male subjects than in female group. Using a cut-off level of 1.08 µmol/spot 20 h, sensitivity and specificity were 100 and 98 %. The linearity of test was proved and we showed that within-run and between run precision were 5.6 and 14.66 %. Measurement of α-l-Iduronidase activity in DBS samples is an accurate test for diagnosis of MPS I and because of its rapid shipping and simplicity to keeping, DBS-based enzyme activity could be considered as a useful diagnostic tool in this disease. 相似文献
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Hossein Motamedi Esmaeil Darabpour Mahnaz Gholipour Seyyed Mansour Seyyed Nejad 《Journal of Zhejiang University. Science. B》2010,11(7):506-511
Brucellosis, a zoonosis caused by four species of brucella, has a high morbidity. Brucella melitensis is the main causative agent of brucellosis in both human and small ruminants. As an alternative to conventional antibiotics,
medicinal plants are valuable resources for new agents against antibiotic-resistant strains. The aim of this study was to
investigate the usage of native plants for brucellosis treatment. For this purpose, the anti-brucella activities of ethanolic
and methanolic extracts of Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, Plantago ovata, Cordia myxa, and Crocus sativus were assessed. The activity against a resistant Br. melitensis strain was determined by disc diffusion method at various concentrations from 50–400 mg/ml. Antibiotic discs were also used
as a control. Among the evaluated herbs, six plant (Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, and Crocus sativus) showed anti-brucella activity. Oliveria decumbens was chosen as the most effective plant for further studies. A tested isolate exhibited resistance to tetracycline, nafcillin,
oxacillin, methicillin, and colistin. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC)
values for Oliveria decumbens against resistant Br. melitensis were the same (5 mg/ml), and for gentamicin they were both 2 mg/ml. Time-kill kinetics for a methanolic extract of Oliveria decumbens was 7 h whereas for an ethanolic extract it was 28 h. Also, Oliveria decumbens extracts showed a synergistic effect in combination with doxycycline and tetracycline. In general, the similar values of
MIC and MBC for Oliveria decumbens suggest that these extracts could act as bactericidal agents against Br. melitensis. In addition to Oliveria decumbens, Crocus sativus and Salvia sclarea also had good anti-brucella activity and these should be considered for further study. 相似文献
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Objective: To report a mathematical function that characterizes the double-pass line spread function (LSF) of the human eye. Determining analytical functions that represent the double-pass LSF is important because it allows modeling the optical performance of the eye. Methods: Optical section retinal images, generated in normal human eyes using a modified slit-lamp biomicroscope, were analyzed to derive the double-pass LSF by plotting the intensity distribution of laser light reflected/ scattered from the vitreoretinal interface. Three mathematical functions (Lorentzian, Gaussian, exponential) were fitted to the double-pass LSF and the root mean square error (RMSE) was calculated to provide a measure of the goodness of fit. Results: The Lorentzian function provided the best representation of the double-pass LSF of normal human eyes. The full width at half maximum (FWHM) of the Lorentzian fitted curve was positively correlated with age, indicating that the double-pass LSF broadens with age. Furthermore, the goodness of fit of the Lorentzian function was significantly better in younger subjects as compared with older subjects, suggesting that the fitted function to the double-pass LSF may vary according to age. Conclusion: The results demonstrate an age-related change in the double-pass LSF width and the goodness of fit of the Lorentzian function. 相似文献
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Abbas Moallem Mahnaz Moallem 《International Journal of Disability, Development & Education》1998,45(1):17-33
The Specialised Vocational Training Institutions (IMPro) in France have recently entered into a difficult, but important period of time. During the past few years, IMPro have faced increasing demand from the public and government to provide quality service to adolescents with intellectual disabilities. IMPro have been also faced with the challenge of being accountable for the low employment rate of adolescents with intellectual disability and their failure to obtain and/or maintain jobs due to lack of technical and social skills. The purpose of the present work was to respond to the increasing and urgent needs in IMPro by designing and developing a training system to enhance organisational productivity, increase employment rate, and eliminate the problems faced by workers with intellectual disabilities. This article describes the process of organisational changes in IMPro using systems thinking. It discusses the model, methods, tools, and techniques used in designing, developing, implementing, and evaluating the new system of training in IMPro in an attempt to improve the quality, effectiveness, and efficiency of their training programs. 相似文献
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The purpose of this paper is to describe the evaluation results of using an interactive design model for the development of
an online course. Specifically, it examines: (a) how an interactive design model was used to develop collaborative and cooperative
learning activities; (b) how activities were structured to promote the level and quality of communications among students,
as peers, and between students and the instructors; and (c) how students responded to such interactive design model. The paper
also provides information about the delivery process and describes what happened when this interactive model was fully implemented
and used. 相似文献
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Involvement of basolateral amygdala GABAA receptors in the effect of dexamethasone on memory in rats
Khajehpour L Alizadeh-Makvandi A Kesmati M Eshagh-Harooni H 《Journal of Zhejiang University. Science. B》2011,12(11):900-908
In this study we investigated whether GABAA receptors of the basolateral amygdala (BLA) interact with the effect of dexamethasone on the retrieval stage of memory. Adult
male Wistar rats were bilaterally cannulated in the BLA by stereotaxic surgery. The animals were trained in step-through apparatus
by induction of electric shock (1.5 mA, 3 s) and were tested for memory retrieval after 1 d. The time of latency for entering
the dark compartment of the instrument and the time spent by rats in this chamber were recorded for evaluation of the animals’
retrieval in passive avoidance memory. Administration of dexamethasone (0.3 and 0.9 mg/kg, subcutaneously (s.c.)), immediately
after training, enhanced memory retrieval. This effect was reduced by intra-BLA microinjection of muscimol (0.125, 0.250 and
0.500 μg/rat), when administered before 0.9 mg/kg of dexamethasone. Microinjection of bicuculline (0.75 μg/rat, intra-BLA)
with an ineffective dose of dexamethasone (0.1 mg/kg, s.c.) increased memory retrieval. However, the same doses of muscimol
and bicuculline without dexamethasone did not affect memory processes. Our data support reports that dexamethasone enhances
memory retrieval. It seems that GABAA receptors of the BLA mediate the effect of dexamethasone on memory retrieval in rats. 相似文献
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Abbas Abdollahi Sana Panahipour Mahnaz Akhavan Tafti Kelly A. Allen 《Psychology in the schools》2020,57(5):823-832
Academic stress is a commonly reported affective state by high school students that can be accompanied by unwanted and unhelpful short- and long-term implications including a low sense of school belonging. Understanding how academic stress may be alleviated or managed by students is an essential consideration for all schools concerned with student well-being. This study aimed to examine the relationship between academic stress and sense of school belonging, as well as the mediation effect of academic hardiness. Four hundred and five high school students from six schools in Tehran (Iran) were recruited in the study. Data analyses with Structural Equation Modeling revealed that a sense of belonging to school (β = −.38, p < .01) and academic hardiness (β = −.40, p < .01) were significant predictors of academic stress. Bootstrapping analysis showed that academic hardiness acted as a mediator between a sense of belonging to school and academic stress. The findings elucidate the underlying mechanism in how low school belonging may lead to academic stress in high school students. Creating a culture of school belonging and teaching and supporting skills related to academic hardiness should be key considerations for all schools seeking to reduce academic stress experienced by their students. 相似文献
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