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Customer service is an important aspect of virtually all organizations. Thus, many try to find ways to improve it. Web-based live-chat support services are one promising means toward this end. However, such services and their success factors have been rarely studied. This study bridges this gap. It builds on justice and service marketing theories, and examines key factors that drive intentions to continue using web-based live-chat support services and to provide positive word-of-mouth. The results suggest that these outcomes are increased through interactional justice perceptions, which are diminished by the perceived busyness of the service provider. It is also suggested that the latter effect is moderated by the duration of the live-chat session; when the session is long the effect is stronger. Data collected from 86 users of a library web-based live-chat service were analyzed with structural equation modeling (SEM) techniques and support this view. Implications for research and practice are discussed.  相似文献   
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Nanoscale synthetic biology can benefit from programmable nanoliter-scale processing of DNA in microfluidic chips if they are interfaced effectively to biochemical arrays such as microwell plates. Whereas active microvalve chips require complex fabrication and operation, we show here how a passive and readily fabricated microchip can be employed for customizable nanoliter scale pipetting and reaction control involving DNA. This recently developed passive microfluidic device, supporting nanoliter scale combinatorial droplet generation and mixing, is here used to generate a DNA test library with one member per droplet exported to addressed locations on microwell plates. Standard DNA assembly techniques, such as Gibson assembly, compatible with isothermal on-chip operation, are employed and checked using off-chip PCR and assembly PCR. The control of output droplet sequences and mixing performance was verified using dyes and fluorescently labeled DNA solutions, both on-chip and in external capillary channels. Gel electrophoresis of products and DNA sequencing were employed to further verify controlled combination and functional enzymatic assembly. The scalability of the results to larger DNA libraries is also addressed by combinatorial input expansion using sequential injection plugs from a multiwell plate. Hence, the paper establishes a proof of principle of the production of functional combinatorial mixtures at the nanoliter scale for one sequence per well DNA libraries.  相似文献   
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Message appeals are a key component of communication campaigns and an important source of campaign influence. However, research on them is heavily fragmented and it is difficult to generalize findings from the many diverse field studies. Based on a large and unique data set using quantitative and qualitative meta-analyses, this research provides measures of the relative impact of each type of appeal, as well as the major differences among them, and identifies the moderating variables that lead to a better understanding of each. This investigation is based on persuasion research, which provides a framework for understanding the unique characteristics of these advertising appeals. It highlights empirical gaps in the academic literature and acts as a conceptual guide for our research hypotheses. Results reveal weaker effect sizes than those previously reported in the literature and show important differences among appeals that lead to a “hierarchy of appeals.” Specifically, emotional appeals, led by sex and humor, appear to be more effective than fear and rational appeals. The study finds new theoretical and empirical generalizations; some results are counterintuitive and differ from findings generated from single appeal studies. Findings are of theoretical and practical importance.  相似文献   
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This study considers the dilemma faced by employees every time a colleague requests knowledge: should they share their knowledge? We use adaptive cost theory and self-efficacy theory to examine how individual characteristics (i.e., self-efficacy and trait competitiveness) and situational perceptions (i.e., ‘busyness’ and perceived competition) affect knowledge sharing behaviours. A study was conducted with 403 students who completed a problem-solving exercise and who were permitted (but not required) to respond to requests for knowledge from people who were doing the same activity. Our results suggest that people who perceive significant time pressure are less likely to share knowledge. Trait competitiveness predicted perceived competition. This and low task self-efficacy created a sense of time pressure, which in turn led to people feeling ‘too busy’ to share their knowledge when it was requested. Perceived competition was not directly related to knowledge sharing. Implications for research and practitioners are discussed.  相似文献   
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