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K. P. Mishra A. P. Yadav Lilly Ganju 《Indian journal of clinical biochemistry : IJCB》2012,27(4):357-362
The Antarctic continent on the planet Earth is full of environmental extremes. It is considered as natural stress model. Therefore, the present study examined the effect of harsh environment on the certain salivary markers of 28th Indian Antarctic expeditioners. Thirty healthy men and women (median age 36 year; range 22–61 year) participated in this study. Parameters measured were salivary IgA (SIgA), IgM (SIgM), TGF-β and cortisol level at three different time points: (I) before leaving India on 26th October 2008 for base line level; (II) after 1 month on-board journey on 31st January 2009 in Southern Ocean and (III) after 1 month staying at Maitri, Antarctica on 3rd March 2009. Our observation indicated that Ship borne journey and Antarctic environment increased the SIgA levels while that of SIgM level was not altered on-board but decreased by staying for 1 month at Antarctica. No significant alteration was found in the TGF-β and cortisol level at any point of time. The present study concluded that ship borne journey and Antarctic environment may induce the SIgA level while SIgM level decreased in environmental extremes of Antarctica. 相似文献
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Potential immunomodulation effect of the extract of Nigella sativa on ovalbumin sensitized guinea pigs 总被引：1，自引：0，他引：1
Boskabady MH Keyhanmanesh R Khameneh S Doostdar Y Khakzad MR 《Journal of Zhejiang University. Science. B》2011,12(3):201-209
Several different pharmacological effects have been described for Nigella sativa (Siah-Daneh), including an anti-inflammatory effect. In the present study, the effect of the extract of N. sativa on lung pathology and blood interleukin-4 (IL-4) and interferon-γ (IFN-γ) of sensitized guinea pigs was examined. Three groups
(n=8 for each group) of guinea pigs sensitized to ovalbumin (OA) were given drinking water alone, and drinking water containing
low and high concentrations of the plant extract, respectively. The animals of the control group (n=8) were treated with saline instead of OA and were given drinking water. The pathological changes of the lung, including
infiltration of eosinophils and lymphocytes, local epithelial necrosis, the presence of oedema, thickening of the basement
membrane, smooth muscle layer hypertrophy, mucosal secretion, and the presence of mucosal plug, and blood IL-4 and IFN-γ of
sensitized guinea pigs were evaluated. The lungs of the sensitized group showed significant pathological changes (P<0.001). Blood IL-4 and IFN-γ were increased in sensitized animals compared to the controls (P<0.01 and P<0.001, respectively). Treatment of sensitized animals with the extract led to a significant decrease in pathological changes
of the lung (P<0.01 to P<0.001), except for the oedema in the sensitized group treated with low concentration of the extract, but an increased IFN-γ.
These results confirm a preventive effect of N. sativa extract on lung inflammation of sensitized guinea pigs. 相似文献
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Shiva Krishna Katkam Liza Rajasekhar Fathima S. D. Tasneem Vijay Kumar Kutala 《Indian journal of clinical biochemistry : IJCB》2021,36(1):59
Systemic lupus erythematosus (SLE) is an inflammatory autoimmune disease which is characterized by dysregulation of various cytokines propagating the inflammatory processes that is responsible for tissue damage. Tumor necrosis factor alpha (TNF-α) is one of the most important immunoregulatory cytokines that has been implicated in the different autoimmune diseases including SLE. Two hundred and two patients with SLE and 318 controls were included in the study. The TNF-α gene promoter region (from − 250 to − 1000 base pairs) was analyzed by direct Sanger’s DNA sequencing method to find promoter variants associated with South Indian SLE patients. We have analyzed six TNF-α genetic polymorphisms including, − 863C/A (rs1800630), − 857C/T (rs1799724), − 806C/T (rs4248158), − 646G/A (rs4248160), − 572A/C (rs4248161) and − 308G/A (rs1800629) in both SLE patients and controls. We did not find association of TNF-α gene promoter SNPs with SLE patients. However, the − 863A (rs1800630) allele showed association with lupus nephritis phenotype in patients with SLE (OR: 1.62, 95%CI 1.04–2.53, P = 0.034). We found serum TNF-α level was significantly elevated in SLE cases as compared to control and found no association with any of the polymorphisms. The haplotype analysis revealed a significant protective association between the wild TNF-α alleles at positions − 863C, − 857C, − 806C, − 646G, − 572A and − 308G (CCCGAG) haplotype with lupus nephritis phenotype (OR 0.53, 95% CI 0.35–0.82, P = 0.004). Additionally, the TNF-α − 863 C/A (rs1800630) polymorphism and HLA-DRB1*07 haplotype showed significant differences between SLE patients and controls (OR 4.79, 95% CI 1.73–13.29, P = 0.0009). In conclusion, TNF-α − 863A allele (rs1800630) polymorphism is associated with increased risk of nephritis in South Indian SLE patients. We also found an interaction between HLA-DRB1*07 allele with TNF-α − 863 C/A promoter polymorphism giving supportive evidence for the tight linkage disequilibrium between TNF-α promoter SNPs and MHC class II DRB1 alleles. 相似文献
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Zi-huan ZHENG Ying HAN Shi-ying YOU Zuo CHEN Xiao-dong ZHENG 《Journal of Zhejiang University. Science. B》2019,(7):576-587
目的:研究美国豆芋(Apios americana)对产后恢复过程,特别是子宫复旧的影响.创新点:开发针对产后恢复的新食品.方法:将雌雄大鼠配种交配,对照组除外(没有交配的雌性大鼠,基础日粮,n=6).分娩后,将雌性大鼠分为五组:基础饮食(模型组,n=6);基础饮食+灌胃给药5.4g/kg的产妇康颗粒(阳性组,n=6);含10%豆芋粉末的基础日粮(低剂量组,n=6);含20%豆芋粉末的基础日粮(中剂量组,n=6);含40%豆芋粉末的基础日粮(高剂量组,n=6).实验结束后,称量子宫和脾脏质量,计算子宫和脾脏指数.并测量血清内激素和细胞因子水平,研究子宫的组织病理学变化,检测基质金属蛋白酶和抑制剂在子宫中的表达.结论:豆芋干预减少了产后子宫指数并调节了激素浓度,加速了子宫形态复旧的过程,减轻了分娩引起的炎症反应.同时发现,转化生长因子β也受豆芋调节.豆芋干预使得基质金属蛋白酶显著下调,其抑制剂则显著上调,这表明豆芋增加了胶原清除过程的速度. 相似文献
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Jyoti Shekhawat Kavya Gauba Shruti Gupta Purvi Purohit Prasenjit Mitra Mahendra Garg Sanjeev Misra Praveen Sharma Mithu Banerjee 《Indian journal of clinical biochemistry : IJCB》2021,36(4):440
COVID-19 has emerged as a global pandemic. It is mainly manifested as pneumonia which may deteriorate into severe respiratory failure. The major hallmark of the disease is the systemic inflammatory immune response characterized by Cytokine Storm (CS). CS is marked by elevated levels of inflammatory cytokines, mainly interleukin-6 (IL-6), IL-8, IL-10, tumour necrosis factor-α (TNF-α) and interferon-γ (IFN-γ). Of these, IL-6 is found to be significantly associated with higher mortality. IL-6 is also a robust marker for predicting disease prognosis and deterioration of clinical profile. In this review, the pivotal role played by IL-6 in the immuno-pathology of COVID-19 has been illustrated. The role of IL-6 as a pleiotropic cytokine executing both pro and anti-inflammatory activities has been reviewed. ADAM 10, a metalloproteinase switches the anti-inflammatory pathway of IL-6 to pro inflammatory hence blocking the action of ADAM 10 could be a new therapeutic strategy to mitigate the proinflammatory action of IL-6. Furthermore, we explore the role of anti-IL6 agents, IL-6 receptor antibodies which were being used for autoimmune diseases but now are being repurposed for the therapy of COVID-19. 相似文献
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Objective: To explore the characteristics of NF-κB activation in the progress of pancreatitis, the relationship with expression of TNF-α in the inflammatory reaction, and prevent the exacerbation of pancreatitis by using NAC. Method: Forty-eight rats were divided into three groups: therapy (group C), pancreatitis (group B) and control (group A). NAC served as the inhibitor of NF-κB activation. In the time intervals of 1.5, 3.0, 6.0, 12.0 hour, NF-κB activation was detected with flow cytometry (FCM) and the expression of TNF-α mRNA and protein with in situ hybridization (ISH) and enzyme-linked immuno-sorbent assay (ELISA) respectively. Meanwhile, the level of lipase and amylase in the serum was assayed and the pathological change was evaluated. Result: NF-κB activation in the pancreatitis group was higher than that in the control group (P<0.01), peaked at 3 hours, and was depressed by the inhibitor of NF-κB, NAC. The expression of TNF-α as well as the level of lipase and amylase in the serum also rose synchronously with activation of NF-κB. In contrast to group A, it was significantly different (P<0.01) in group B. After using NAC in group C, all of these values were decreased and the inflammatory reaction in the pancreas abated evidently. The pathology changes of the pancreas were shown to be alleviated in group C. Conclusion: First, NF-κB activity is intensively initiated in the course of pancreatitis and shown to have closely relationship with the release of cytokines. Second, use of NAC markedly depressed NF-κB activation. TNF-α expression is down regulated by cytokines. It is suggested that NAC probably acts as a useful agent for treatment of pancreatitis by indirectly inhibiting activation of NF-κB. 相似文献
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Acute phase reaction and acute phase proteins 总被引：18，自引：0，他引：18
Gruys E Toussaint MJ Niewold TA Koopmans SJ 《Journal of Zhejiang University. Science. B》2005,6(11):1045-1056
INTRODUCTION The first reaction of the body to immunological stress is the innate, non-specific response preceding specific immune reactions. The acute phase response (APR) is a prominent systemic reaction of the or- ganism to local or systemic disturbances in its ho- meostasis caused by infection, tissue injury, trauma or surgery, neoplastic growth or immunological disor- ders (Gordon and Koy, 1985; Gruys et al., 1999). At the site of invasion by a micro-organism and the place of tissu… 相似文献
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GRUYS E TOUSSAINT M.J.M NIEWOLD T.A KOOPMANS S.J 《Journal of Zhejiang University. Science. B》2005,6(11)
A review of the systemic acute phase reaction with major cytokines involved, and the hepatic metabolic changes,negative and positive acute phase proteins (APPs) with function and associated pathology is given. It appears that APPs represent appropriate analytes for assessment of animal health. Whereas they represent non-specific markers as biological effect reactants,they can be used for assessing nutritional deficits and reactive processes, especially when positive and negative acute phase variables are combined in an index. When such acute phase index is applied to separate healthy animals from animals with some disease, much better results are obtained than with single analytes and statistically acceptable results for culling individual animals may be reached.Unfortunately at present no cheap, comprehensive and easy to use system is available for assessing various acute phase proteins in serum or blood samples at the same time. Protein microarray or fluid phase microchip technology may satisfy this need;and permit simultaneous analysis of numerous analytes in the same small volume sample and enable integration of information derived from systemic reactivity and nutrition with disease specific variables. Applying such technology may help to solve health problems in various countries not only in animal husbandry but also in human populations. 相似文献