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采用活体接种的方法,比较致病疫霉在辣椒和番茄上的致病性差异;结果表明致病疫霉在两种寄主上都能侵染,但是在番茄上的致病能力远远强与辣椒上的致病能力. 相似文献
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INTRODUCTION Plants have defense mechanisms against patho-gen infection by inducing systemic resistance in re-sponse to localized pretreatment with biological cotrol agents, thus making them resistant to subsequpathogen infection (Caruso et al., 1999; Hammschmidt, 1999; Mohammadi and Kazemi, 2002; yada et al., 1995; Pozo et al., 2002; Ray et al., 199Biological control of plant pathogens has receivmuch more attention. It is well known that plants amicroorganisms symbiosis is a defense m… 相似文献
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寡雄腐霉和木霉对辣椒疫霉拮抗作用的初步研究 总被引:1,自引:0,他引:1
何美仙 《金华职业技术学院学报》2002,2(2):25-27
本文通过对峙培养测定、菌间交互作用机制观察及拮抗菌培养滤液对疫霉菌生长影响的测定,探讨寡雄腐霉Pythium oligandrum和木霉BT-95对辣椒疫病菌Phytophthora capsici是否有拮抗作用及其作用机制。 相似文献
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抗甲霜灵烟草黑胫病菌突变株的交互抗药性研究 总被引:3,自引:0,他引:3
采用菌落生长速率法,测定了2种保护性杀菌剞和4种内吸性杀菌剂对烟草黑胫病菌敏感菌株和4个抗甲霜灵突变株的EC50值,测定了抗甲霜灵烟草黑胫病菌突变株对5种杀菌剂的交互抗性。结果表明:抗甲霜灵烟草黑胫病菌突变株对代森锰锌有负交互抗性现象,对百菌清、霜脲氰、乙磷铝和霜霉威无交互抗性。 相似文献
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通过单因子实验,筛选得到了适合大豆疫霉拮抗细菌B048液体发酵的最佳碳源为玉米粉,最佳氮源为豆粕,最佳无机盐离子为氯化钠。通过正交实验确定了该培养基的最佳配比为:玉米粉5g/L、豆粕15g/L、氯化钠10g/L。在确定适合该菌株的培养基组分基础上,对其培养条件进行优化,结果表明:初始pH值为6.0,培养时间为48h,转速为180r/min时为该菌株的最佳培养条件。 相似文献
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Zheng Hu-zhe Cui Chun-lan Zhang Yu-ting Wang Dan Jing Yu Kim Kil Yong 《Journal of Zhejiang University. Science. B》2005,6(8):778-786
The activities of enzymes responsible for lignification in pepper, pre-inoculation with arbuscular mycorrhizal (AM) fungus
ofGlomus intraradices and/or infection with pathogenic strain ofPhytophthora capsici, and the biological control effect ofG. intraradices on Phytophthora blight in pepper were investigated. The experiment was carried out with four treatments: (1) plants pre-inoculated
withG. intraradices (Gi), (2) plants pre-inoculated withG. intraradices and then infected withP. capsici (Gi+Pc). (3) plants infected withP. capsici (Pc), and (4) plants without any of the two microorganisms (C). Mycorrhizal colonization rate was reduced by about 10% in
pathogen challenged plants. Root mortality caused by infection ofP. capsici was completely eliminated by pre-inoculation with antagonisticG. intraradices. On the ninth day after pathogen infection, Peroxidase (POD) activity increased by 116.9% in Pc-treated roots but by only
21.2% in Gi+Pc-treated roots, compared with the control, respectively. Polyphenol oxidase (PPO) and Phenylalanine ammonia-lyase
(PAL) activities gradually increased during the first 3 d and dramatically decreased in Pc-treated roots but slightly decreased
in Gi+Pc-treated roots, respectively. On the ninth day after pathogen infection, PPO and PAL decreased by 62.8% and 73.9%
in Pc-treated roots but by only 19.8% and 19.5% in Gi+Pc-treated roots, compared with the control, respectively. Three major
POD isozymes (45000, 53000 and 114000) were present in Pc-treated roots, while two major bands (53000 and 114000) and one
minor band (45000) were present in spectra of Gi+Pc-treated roots, the 45000 POD isozyme was significantly suppressed byG. intraradices, suggesting that the 45000 POD isozyme was induced by the pathogen infection but not induced by the antagonisticG. intraradices. A 60000 PPO isozyme was induced in Pc-treated roots but not induced in Gi+Pc-treated roots. All these results showed the
inoculation of antagonisticG. intraradices alleviates root mortality, activates changes of lignification-related enzymes and induces some of the isozymes in pepper
plants infected byP. capsici. The results suggested thatG. intraradices is a potentially effective protection agent againstP. capsici.
Project supported by Korea Science and Engineering Foundation (KOSEF) through the Agricultural Plants Stress Research Center
(APSRC) at Chonnam National University, Korea 相似文献
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