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1.
Proteins and glutathione (GSH) levels were estimated in the experimental groups. Levels of both proteins and glutathione in
the control and the treated groups were found to be significantly decreased compared to the normal group. The results show
that supplementation of riboflavin has beneficial effect by alleviating the oxidant injuries to GSH and proteins, which are
involved in the maintenance of transparency and protection of thiol groups of the lens. Thus, riboflavin has an active role
in the lens metabolism and has a potential to improve the metabolic activity of the cataractous lenses. 相似文献
2.
目的:观察CoQ10补充对青少年运动员血浆抗氧化系统的影响.方法:24名进行耐力训练的男性青少年游泳运动员按有氧运动能力单盲随机分为Q组及P组,分别补充CoQ10 100mg/d或安慰剂28d.结果:(1)补充后Q组血浆CoQ10浓度显著增高,且显著高于P组;(2)恒定负荷运动后受试者的总体血浆CoQ10浓度较运动前显著降低;(3)补充后Q组安静血乳酸浓度的升高程度显著低于P组;(4)补充后Q组安静状态的血浆MDA水平无显著改变,且显著低于P组;(5)补充后Q组运动后即刻较安静状态血浆SOD活性的增加程度显著低于P组;(6)补充后安静状态下Q组血浆GPX活性的提高程度显著低于P组;(7)补充前后Q组与P组血浆CAT活性变化无组间差异.结论:急性耐力运动中机体对血浆CoQ10的需求增加;CoQ10补充可降低进行大强度耐力训练的青少年游泳运动员的脂质过氧化程度并能改善机能状态;CoQ10补充在急、慢性运动状态下协同不同抗氧化酶发挥抗氧化作用. 相似文献
3.
建立微芯片电泳-电化学检测技术分析细胞内还原型（GSH）和氧化型（GSSG）谷胱甘肽的新方法. 分别考察缓冲液pH值、缓冲液浓度、SDS浓度、分离电压、进样时间、检测电位等因素对GSH、GSSG分离检测的影响. 在最优实验条件下,在3 min内实现GSH和GSSG的有效分离和检测. GSH和GSSG的线性范围分别为5.0~200.0 μmol/L和2.0~100.0 μmol/L（R2>0.99）,最低检测限（S/N≥3）分别为4.87 μmol/L和1.98 μmol/L. 最终将所建立的方法应用于细胞样品中2种物质含量的测定,结果令人满意. 相似文献
4.
耗竭运动对大鼠心肌线粒体功能的影响 总被引：4，自引：0，他引：4
采用耗竭运动方式,观察了大鼠运动后心肌线粒体脂质过氧化水平、超氧化物岐化酶活性和还原型谷胱甘肽含量的变化。发现运动后即刻心肌线粒体脂质过氧化水平显著升高,超氧化物岐化酶活性和还原型谷胱甘肽含量显著下降。结果提示耗竭运动时,心肌线粒体内源性自由基的产生是耐力性运动疲劳和运动损伤的原因之一。 相似文献
5.
常压模拟高住低练和低住高练对大鼠肝脏谷胱甘肽抗氧化系统影响的比较 总被引：6，自引：0，他引：6
目的:探讨常压模拟高住低练和低住高练两种低氧训练方法对SD大鼠肝脏谷胱甘肽抗氧化系统的影响。方法:将成年雄性SD大鼠40只随机分为平原安静组(A)、平原运动组(B)、常压低住高练组(C)和常压高住低练组(D),每组10只,4周后测定大鼠肝脏谷胱甘肽抗氧化系统及相关指标。结果:低住高练组和高住低练组大鼠肝脏GSH、GSH-PX、T-AOC最高,MDA含量最低,两组低氧训练组间没有显著性差异,但与其它组相比较,差异显著(P<0.05)。结论:低住高练和高住低练此两种训练方法均能提高机体谷胱甘肽抗氧化系统能力。 相似文献
6.
Gora Dadheech Praveen Sharma Shiv Gautam 《Indian journal of clinical biochemistry : IJCB》2012,27(3):278-283
Reactive oxygen species (ROS) formed in various metabolic reactions cause unlimited damage by attacking and oxidizing the macromolecules. An arsenal of antioxidant substances neutralizes these ROS at various sites of their metabolic cascade, and if disequilibrium exists between the pro and antioxidant system, oxidative stress persists. The present study was undertaken in schizophrenia, to highlight the response and role of some endogenous antioxidants viz. reduced glutathione (GSH), bilirubin, total proteins, albumin and uric acid in scavenging the ROS. The effect of severity of disease, age factor, and substance abuse was also studied. In all, 50 schizophrenics and 50 age and sex-matched controls were enrolled in the present study. Fasting blood samples were drawn for estimating malondialdehyde (MDA), GSH, bilirubin, total proteins, albumin and uric acid in both the groups. The results were statistically analyzed by Z-test and correlated using correlation coefficient (r). The study shows reduction in MDA levels and decline in the level of endogenous antioxidants, but within the normal range. Chronic schizophrenics were at a higher risk of oxidative stress and age and substance abuse seems to worsen the situation. 相似文献
7.
K. Poornima M. Cariappa K. Asha H. P. Kedilaya M. Nandini 《Indian journal of clinical biochemistry : IJCB》2003,18(2):197-205
An adequate antioxidant reserve which is usually present in a vegetarian diet is associated with higher life expectancy. On
the other hand habitual consumption of fish is associated with lower risk of cardiovascular diseases which is attributed to
the polyunsaturated fatty acids (PUFA) present in it. However the PUFA are readily susceptible to oxidation leading to the
formation of free radicals which is known to be involved in the causation of several diseases.
The oxidant-antioxidant status of 23 vegetarians and 22 fish eaters was studied by determining the plasma lipid peroxides
measured as malondialdehyde (MDA) and the antioxidants, viz. glutathione (GSH), ascorbic acid, ceruloplasmin and uric acid.
Results show that the ascorbic acid values were significantly higher in vegetarians. In addition, MDA correlated negatively
with ascorbic acid only in vegetarians. However, correlation of MDA with glutathione showed a significant negative correlation
only in fish eaters. There was no statistically significant difference in the MDA, GSH, ceruloplasmin and uric acid levels.
The mechanism behind these findings are not clear and needs to be explored. 相似文献
8.
研究发现当两种不同的玉米幼苗在28℃干旱下处理4d后，干旱处理的幼苗的存活率比未干旱处理的幼苗低，这说明此干旱条件使玉米幼苗受到伤害而降低其存活率．而且，在干旱胁迫过程中，干旱处理过的幼苗的谷胱甘肽还原酶活性发生了变化，在胁迫初期谷胱甘肽还原酶活性上升，而在胁迫后期活性下降．测定酶液中的蛋白质发现，干旱胁迫使蛋白质含量降低．这些结果表明，在干旱初期谷胱甘肽还原酶参与抗旱性的构建，后期由于蛋白质的降解而使谷胱甘肽还原酶活性降低． 相似文献
9.
M. B. Cooper D. A. Jones R. H. T. Edwards C. Corbucci G. Montanari C. Trevisani 《Journal of sports sciences》2013,31(2):79-87
Carnitine is an essential co‐factor in the catabolism of fats as an energy source. The primary purpose of this study was to investigate the effect of running a marathon on the metabolism of carnitine by endurance‐trained athletes, and to evaluate the effect of carnitine administration on the performance of such exercise. The effects of marathon running on mitochondrial enzymes and cellular anti‐oxidants were also examined to assess whether the expression of these activities is altered by exercise. Subjects were 10 experienced male marathon runners aged between 19 and 25 years. Running a marathon caused a fall in the plasma content of unesterified carnitine (37%) and an increase in the level of acetylcarnitine present (288%). Loading of the athletes with L‐carnitine for 10 days before running a marathon abolished the exercise‐induced fall in plasma‐free carnitine (P<0.05) whilst amplifying the production of acetylcarnitine (P<0.05). Carnitine loading of the athletes studied made no detectable improvement in performance of the marathon (P > 0.05). Cytochrome oxidase, succinate cytochrome C reductase and Superoxide dismutase activities present in skeletal muscle were unaltered by marathon running. However, such exercise caused a large increase in the tissue content of oxidized glutathione (189%) at the expense of reduced glutathione (–18%). 相似文献
10.
摇瓶培养酵母生产谷胱甘肽的研究 总被引：1，自引：0，他引：1
在175rpm,30℃条件下摇瓶培养C、U、B 101 GSH积累菌种,研究了pH、接种量、菌龄及混合氨基酸、ATP对生产GSH的影响。研究结果表明,培养基中K_2HPO_4·3H_2O和KH_2PO_4的适宜浓度均为1g·L~(-1),此时初始pH为7.26;最佳接种量为10％,最佳接种菌龄为24hr;由谷氨酸、半胱氨酸和甘氨酸组成的混合氨基酸中半胱氨酸为关键氨基酸,它抑制菌体生长,但能提高GSH含量;ATP会促进菌体生长和提高GSH含量,但效果不很明显。上述生产条件使得CSH含量为1.74％,菌体重5g·L~(-1),GSH总量85mg·L~(-1)。 相似文献