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Ⅱ型糖尿病大鼠运动后骨骼肌IRS-2、GLUT4含量及IRS-2磷酸化变化的时间特征 总被引：1，自引：0，他引：1
为了解运动后大鼠肌细胞IRS-2、GLUT4蛋白含量及IRS-2磷酸化的动态变化及时间特征。制备II型糖尿病大鼠模型。将SD大鼠分为正常对照组、糖尿病对照组和糖尿病运动组。用Western blot免疫印迹法检测运动后IRS-2、GLUT4蛋白含量及IRS-2磷酸化程度。结果得出:与糖尿病对照组相比,运动后1 h、3 h组、6 h、12 h、24 h和48 h股四头肌IRS-2蛋白含量分别升高6.8%、23.7%(P<0.01)、20.7%(P<0.01)、10%、5.4%和2.7%;GLUT4蛋白含量分别显著升高17.3%、32.7%、42.3%、46.2%、51.9%和45%(P<0.01);IRS-2磷酸化分别升高49%、51.4%、45.1%、43.1%、21.6%(P<0.01)和9.8%(P<0.05)。结论:一次性中等强度的运动对IRS-2、GLUT4含量和IRS-2磷酸化产生显著的影响,其中GLUT4含量和IRS-2磷酸化升高的效应可持续到运动结束的48 h。 相似文献
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题目：PGC-1α在CH1细胞中通过ATP和ROS调控细胞周期 总被引：2，自引：0，他引：2
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磷脂分子不仅作为线粒体膜的结构成份，而且参与膜功能活动调节。本文综述在某些病理因素和运动因素作用下，膜磷脂含量改变对线粒体氧化磷酸化的影响。 相似文献
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呼吸链电子传递: 线粒体氧化磷酸化偶联的重要限速步骤 总被引：2，自引：0，他引：2
线粒体氧化磷酸化解偶联和ＡＴＰ合成的降低可能是运动性疲劳的重要线粒体膜分子机制之一。作为这一进程的重要限速步骤,呼吸链电子传递可能与运动过程中线粒休氧化磷酸化解偶联密切相关。并由此提出“呼吸链电子传递可能是和长时间运动中线粒磷酸化的重要限速步骤”假说。 相似文献
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Sitthichai IAMSAARD Thawatchai PRABSATTROO Wannisa SUKHORUM Supaporn MUCHIMAPURA Panee SRISAARD Nongnut UABUNDIT Wipawee THUKHAMMEE Jintanaporn WATTANATHORN 《Journal of Zhejiang University. Science. B》2013,14(3):247-252
Objective:To investigate the effect of Anethum graveolens(AG) extracts on the mounting frequency,histology of testis and epididymis,and sperm physiology.Methods:Male rats induced by cold immobilization before treating with vehicle or AG extracts [50,150,and 450 mg/kg body weight(BW)] via gastric tube for consecutive 1,7,and 14 d were examined for mounting frequency,testicular phosphorylation level by immunoblotting,sperm concentration,sperm acrosome reaction,and histological structures of testis and epididymis,respectively.Results:AG(50 mg/kg BW) significantly increased the mounting frequency on Days 1 and 7 compared to the control group.Additionally,rat testis treated with 50 mg/kg BW AG showed high levels of phosphorylated proteins as compared with the control group.In histological analyses,AG extract did not affect the sperm concentration,acrosome reaction,and histological structures of testis and epididymis.Conclusions:AG extract enhances the aphrodisiac activity and is not harmful to sperm and male reproductive organs. 相似文献
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探讨力竭性运动引起的氧化应激对肠功能的影响及运动性肠功能紊乱的原因,32只SD雄性大鼠随机分为4组:即对照组(C);运动后即刻组EX;运动后30 min组(EX30);运动后60 min组(EX60),测定力竭性游泳后不同时相,肠组织匀浆MDA、游离巯基(Free-SH)和ATP含量。结果显示,运动后肠组织MDA含量在运动后30 min,60 min显著性增加(P0.01);运动后Free-SH含量在运动后30min(P0.05)和运动后60 min后(P0.01)显著下降;运动后30 min组ATP含量显著下降(P0.01)。结果提示,运动源性自由基产生增加,使得肠组织中游离巯基被氧化,导致ATP含量下降,可能是造成运动性肠功能紊乱的重要因素之一。 相似文献
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Sitthichai IAMSAARD Jaturon BURAWAT Pipatpong KANLA Supatcharee ARUN Wannisa SUKHORUM Bungorn SRIPANIDKULCHAI Nongnut UABUNDIT Jintanaporn WATTATHORN Wiphawi HIPKAEO Duriya FONGMOON Hisatake KONDO 《Journal of Zhejiang University. Science. B》2014,15(6):548-555
Background
Ketoconazole (KET), an antifungal drug, has adverse effects on the male reproductive system. Pre-treatments with antioxidant plant against testicular damage induced by KET are required. The flowers of Clitoria ternatea (CT) are proven to have hepatoprotective potential. However, the protective effect on KET-induced testicular damage has not been reported.Objective
To investigate the protective effect of CT flower extracts with antioxidant activity on male reproductive parameters including sperm concentration, serum testosterone level, histopathology of the testis, and testicular tyrosine phosphorylation levels in rats induced with KET.Methods
The antioxidant activity of CT flower extracts was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Male rats were treated with CT flower extracts (10, 50, or 100 mg/kg BW) or distilled water via a gastric tube for 28 d (preventive period: Days 1–21) and induced by KET (100 mg/kg BW) via intraperitoneal injection for 7 d (induction period: Days 22–28). After the experiment, all animals were examined for the weights of the testis, epididymis plus vas deferens and seminal vesicle, serum testosterone levels, sperm concentration, histological structures and diameter of testis, and testicular tyrosine phosphorylation levels by immunoblotting.Results
The CT flower extracts had capabilities for DPPH scavenging and high reducing power. At 100 mg/kg BW, the extract had no toxic effects on the male reproductive system. Significantly, in CT+KET groups, CT flower extracts (50 and 100 mg/kg BW) alleviated the reduction of reproductive organ weight parameters, testosterone levels, and sperm concentration. In addition, CT flower extracts gave protection from testicular damage in KET-induced rats. Moreover, in the CT100+KET group, CT flower extracts significantly enhanced the expression of a testicular 50-kDa tyrosine phosphorylated protein compared with that of other groups.Conclusions
C. ternatea flower extracts possessing antioxidant activity are not harmful to the male reproductive system and can protect against testicular damage in KET-induced rats. 相似文献10.
对长期运动训练大鼠红细胞带3蛋白磷酸化的变化进行研究,为运动训练导致红细胞溶血增加提供理论依据。实验结果表明长期大强度递增负荷跑台运动引起运动组大鼠红细胞参数显著降低,呈现运动性低血色素状态;运动组红细胞膜带3蛋白磷酸化与安静组比较非常显著性增加(P<0.01),表明长期大强度递增负荷跑台运动导致红细胞膜结构和功能受到严重影响,从而影响红细胞膜骨架,进而使红细胞溶血增加。 相似文献