运动、氧化应激与DNA损伤和修复   总被引：4，自引：0，他引：4  

常波 《沈阳体育学院学报》2004,23(6):756-757,788

在分子和基因水平上对运动时氧化应激损伤、氧化应激与DNA损伤以及DNA损伤和修复等几方面关系进行探讨,旨在探讨运动疲劳和损伤发生的分子生物学基础,并对未来运动医学研究的发展趋势进行展望。  相似文献   

骨骼肌基因转移系统的建立     

陆爱云  文立  曾凡辉  陈立  邹蓓艳  卢大儒  薛京伦 《上海体育学院学报》2002,26(4):43-47

以EGFP为标记基因，利用重新构建的pAV-EGFP质粒，采用直接注射方法，进行了离体和在体基因转移研究。实验结果显示，经脂质体包裹后，质粒可以有效地进行细胞转移；DNA表达载体肌肉直接注射后，利用共聚焦显微镜可以在注射点周围的肌细胞观察到绿色荧光蛋白；而在对侧肢PCR和共聚焦显微镜的结果均为阴性。实验结果表明，肌肉直接注射DNA可以在肌肉得到表达，将会为治疗肌肉损伤带来新的方法。  相似文献   

运动性疲劳分子生物学机制的研究进展   总被引：12，自引：0，他引：12  

徐明  蔡国梁  李若愚 《成都体育学院学报》2005,31(4):91-94,98

从四个方面(细胞内分子、细胞膜分子、细胞外分子和其他广泛存在的分子和离子)综合分析运动性疲劳分子生物学机制的研究进展,以期达到完善运动性疲劳机制理论体系的目的.  相似文献   

DNA生物大分子的动力学特性与生物功能(Ⅱ)     

庞小峰 《商丘师范学院学报》1996,(Z2)

对ＤＮＡ中激发的孤立子进行了分类，同时也研究了它的一些特点．我们着重研究了用此理论解释ＤＮＡ的基本生物功能──复制和转录及遗传与变异等问题．  相似文献   

盐浓度、酸碱度及DNA浓度对增色效应实验的影响   总被引：1，自引：0，他引：1  

曾碧慧  张志刚 《实验室研究与探索》2001,20(1):43-45,50

研究了DNA在不同浓度，不同pH值和不同氯化钠溶液中紫外吸光度的变化，为设计DNA的增色效应实验提供了依据。  相似文献   

芒果基因组DNA的提取方法比较   总被引：3，自引：0，他引：3       下载免费PDF全文

段中岗  庄海波  张嘉铭 《惠州学院学报》2006,26(3):15-17

采用SDS法、改良SDS法、CTAB法、改良CTAB法提取芒果基因组DNA,并对提取的DNA进行质量比较。结果表明:改良CTAB法能较好地去除芒果叶片中的酚类和多糖杂质,提取的DNA母液浓度达767．2 ng/μl, OD260／OD280为1．89,DNA纯度和浓度在四种方法中均为最高。  相似文献   

Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant     

陈建忠  朱海红  刘克洲  陈智 《浙江大学学报(A卷英文版)》2004,5(4):467-471

Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.  相似文献   

Dependence of nucleotide physical properties on their placement in codons and determinative degree     

Berezhnoy AY  Duplij SA 《Journal of Zhejiang University. Science. B》2005,6(10):948-960

INTRODUCTION It is well-known from the genetic code structure that there exists a connection between codons and physical and chemical amino acid properties (Lewin, 1983). Codon properties in turn are defined by physical properties of the nucleotides from which they are compounded (Ratner, 1985; 2000). The most important properties of nucleotides as complex three-dimensional molecules are their dipole moment, heat of formation and energy of the most stable con- formation (Schneider and Be…  相似文献   

DNA甲基化异常与肿瘤发生的研究     

王栋  张维铭 《三明学院学报》2002,19(4):40-44

DNA甲基化是真核生物DNA的正常内源性修饰方式 ,它由DNA甲基化转移酶催化发生。但DNA甲基化异常在肿瘤发生中却起着极为重要的作用 ,它主要表现为抑癌基因高甲基化所致的基因失活和原癌基因低甲基化所致的基因激活 ,引起与细胞增殖、分化相关基因表达异常 ,造成细胞恶变形成肿瘤。文章总结了DNA甲基化异常对肿瘤相关基因表达的影响 ,在肿瘤发生中的可能机制及针对高甲基化的治疗方案  相似文献   

Scaling behavior of nucleotide cluster in DNA sequences     

Cheng J  Tong ZS  Zhang LX 《Journal of Zhejiang University. Science. B》2007,8(5):359-364

In this paper we study the scaling behavior of nucleotide cluster in 11 chromosomes of Encephalitozoon cuniculi Genome. The statistical distribution of nucleotide clusters for 11 chromosomes is characterized by the scaling behavior of P（S） ∝ e^-αs＇ where S represents nucleotide cluster size. The cluster-size distribution P（S1＋S2） with the total size of sequential C-G cluster and A-T cluster S1＋S2 were also studied. P（S1＋S2） follows exponential decay. There does not exist the case of large C-G cluster following large A-T cluster or large A-T cluster following large C-G cluster. We also discuss the relatively random walk length function L（n） and the local compositional complexity of nucleotide sequences based on a new model. These investigations may provide some insight into nucleotide cluster of DNA sequence.  相似文献