| 酸性刺激对大鼠骨骼肌细胞胞浆Ca2+的影响 |
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| 引用本文： | 周锦琳,田野.酸性刺激对大鼠骨骼肌细胞胞浆Ca2+的影响[J].北京体育大学学报,2005,28(9):1201-1203. |
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| 作者姓名： | 周锦琳 田野 |
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| 作者单位： | 1. 北京体育大学,北京,100084
2. 国家体育总局体育科学研究所,北京,100061 |
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| 基金项目： | 北京体育大学校科研和教改项目
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高等学校博士学科点专项科研项目 |
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| 摘 要： | 研究表明,运动引起胞浆Ca2 聚积是骨骼肌疲劳的重要原因.从研究酸性刺激时胞浆Ca2 的变化入手,从细胞水平分析急性运动可能对骨骼肌胞浆钙稳态的影响,试图为全面了解运动性疲劳的产生机制提供理论依据.实验中以Fluo-3-AM为Ca2 荧光指示剂,通过激光扫描共聚焦显微镜系统对用体外组织分离和培养的方法获得的单个的骨骼肌细胞胞浆Ca2 的荧光强度及其动态变化进行观察.结果发现:骨骼肌细胞受到酸性刺激时,胞浆Ca2 稳态被破坏.其总体变化趋势是先略有增加后迅速减少,直至在比正常低的水平上形成新的动态平衡.钙波的变化既有振幅的变化,也有频率的变化.这可能提示:运动后,由于内环境酸性代谢产物的堆积,致使胞浆Ca2 稳态破坏,最终引起骨骼肌疲劳.
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| 关 键 词： | 骨骼肌疲劳 酸性刺激 钙稳态 胞浆Ca2 酸性刺激 大鼠骨骼肌 细胞胞浆 影响 Acid Effects Research Skeletal Muscles 代谢产物 内环境 运动后 频率 振幅 钙波 动态平衡 水平 变化趋势 破坏 稳态 骨骼肌细胞 |
| 文章编号： | 1007-3612(2005)09-1201-04 |
| 修稿时间： | 2005年6月20日 |
Research on Effects of Acid on Cytoplasmic Ca2+ Metabolism of Rat Skeletal Muscles |
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| ZHOU Jin-lin,TIAN Ye.Research on Effects of Acid on Cytoplasmic Ca2+ Metabolism of Rat Skeletal Muscles[J].Journal of Beijing Sport University,2005,28(9):1201-1203. |
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| Authors: | ZHOU Jin-lin TIAN Ye |
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| Abstract: | A growing body of literature indicated that the exercise resulted in the decrease of Ca~(2+) transportation capacities. Cytoplasmic Ca~(2+) accumulation can be seen as an important factor that caused skeletal muscle fatigue. Consequently, a systematic study was conducted to observe the effect of acid on cytoplasmic Ca~(2+) in order to analyze how acute exercise influenced the cytoplasmic Ca~(2+) homeostasis. The fluorescent indicator, Fluo-3-AM and the laser confocal microscope system were used for measurements of cytoplasmic Ca~(2+) concentration and its time course of individual skeletal muscle cells, which were obtained by the isolation culture techniques. This study was conducted at the cell level to explore the mechanisms of exercise-induced fatigue, which may provide scientific training with theories foundation. The results indicated that the cytoplasmic Ca~(2+) homeostasis was disrupted when the skeletal muscle cells were in the acid condition(pH=6.9). Cytoplasmic Ca~(2+) initially increased, then decreased, and in the end came into being a new Ca~(2+) homeostasis under the base line. The swing as well as the frequency of cytoplasmic Ca~(2+) wave changed while muscle cells were in acid. It suggested cytoplasmic Ca~(2+) homeostasis disruption due to the accumulation of acidic metabolic products resulted in the muscle fatigue after acute exercise. |
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| Keywords: | muscle fatigue acid Ca~(2+) homeostasis cytoplasmic Ca~(2+) |
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