| 运动对高脂饮食大鼠下丘脑SOCS-3和BDNF以及瘦素抵抗的影响 |
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| 引用本文： | 田雷,陈钢.运动对高脂饮食大鼠下丘脑SOCS-3和BDNF以及瘦素抵抗的影响[J].广州体育学院学报,2013(6):85-89. |
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| 作者姓名： | 田雷 陈钢 |
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| 作者单位： | (东北师范大学体育学院,吉林 长春130024) |
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| 摘 要： | 目的：观察运动对高脂饮食大鼠血清和下丘脑瘦素（Leptin）以及下丘脑 瘦素长型受体（OB-Rb）
、促黑皮素受体4（MC4R）、细胞因子信号转导抑制因子-3（SOCS-3） mRNA表达和脑源性神经营养因子（
BDNF）的影响,探讨适宜运动对高脂饮食大鼠预防肥胖的部分中枢机制。方法：雄性SD鼠24只,随机分为
正常对照组（C组）,高脂模型组（H组）和高脂 60min 运动组（HE组）,每组8只。高脂模型组和高脂
60min 运动组大鼠喂饲高脂饲料,高脂饮食运动组大鼠进行60min的无负重游泳运动。持续10周。结果：
10周运动后,高脂模型组大鼠较正常对照组大鼠体重、Lee''S指数、体脂、脂体比均显著增高(p<0.05),
血清瘦素水平显著升高(p<0.05),下丘脑内OB-Rb、MC4R mRNA表达量显著降低(p<0.05),SOCS-3 mRNA表
达量显著升高(p<0.05),下丘脑BDNF含量显著降低(p<0.05);高脂60min运动组较高脂模型组大鼠体重显
著降低(p<0.01),Lee''S指数、体脂、脂体比均显著降低(p<0.05),血清瘦素水平显著升高(p<0.05),下
丘脑OB-Rb、MC4R、leptin mRNA表达量显著升高(p<0.05),下丘脑SOCS-3 mRNA表达量显著降低(p<0.05)
,下丘脑BDNF含量显著升高(p<0.05)。结论：长期适宜的运动可通过降低下丘脑SOCS-3的表达,增加下丘
脑Leptin 与受体OB-Rb的结合,降低了瘦素抵抗,激活下丘脑含 MC4R 受体的神经元,从而刺激 BDNF 的
释放,从而起到预防高脂饮食引起肥胖的作用。
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| 关 键 词： | 运动 肥胖 细胞因子信号转导抑制因子-3 脑源性神经营养因子 瘦素抵抗 |
| 修稿时间： | 2013/7/18 0:00:00 |
Effect of Swimming Exercise on Hypothalamus SOCS-3, BDNF and Leptin-resistance in Rat with High-fat Diet |
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| TIAN Lei,CHENG Gang.Effect of Swimming Exercise on Hypothalamus SOCS-3, BDNF and Leptin-resistance in Rat with High-fat Diet[J].Journal of Guangzhou Physical Education Institute,2013(6):85-89. |
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| Authors: | TIAN Lei CHENG Gang |
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| Abstract: | Objective: To explore the possible mechanism of exercises in high-fat diet induced
rats with obesity, we observed the serum leptin, the mRNA expression of leptin, OB-Rb, MC4R
and SOCS-3 in hypothalamus, and the content of BDNF in hypothalamus. Method: 24 male
Sprague-Dawley rats were randomly assighned to 3groups (each n=8): control group (C Group),
high-fat model group (HE) and high-fat 60-min exercise training group (HE Group),
respectively. Rats of HE Group swam for 60 min each day without loading. Results: After 10
weeks, to compared with the C group, the body weigh, Lee''s index and the body fat of H group
were obviously increased (P<0.05), the serum leptin was significantly increased (P<0.05); In
hypothalamus, the mRNA expression of OB-Rb and MC4R were dramatically decreased (P<0.05) and
the mRNA expression of SOCS-3 was markedly increased (P<0.05), the concentration of BDNF was
obviously decreased (P<0.05). To compared with the H group, the body weight, Lee''s index and
the body fat of HE group was obviously lower (P<0.01); the serum leptin was significantly
decreased (P<0.05); In hypothalamus, the mRNA expression of OB-Rb, leptin, and MC4R were
dramatically increased (P<0.05), and the mRNA expression of SOCS-3 markedly decreased
(P<0.05), the concentration of BDNF was obviously increased (P<0.05). Conclusion: The
appropriate long-term exercise can ameliorate the "leptin resistance", the possible
mechanisms is: a long-term exercise can decrease the expression mRNA of SOCS-3, then induced
OB-Rb binding to leptin, with its activation to the hypothalamic neurons MC4R that causing a
increase in BDNF production, thereby reducing food intake and stimulating the motor neuron
to increase exercise training, so that increased energy expenditure. |
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| Keywords: | exercise obesity SOCS-3 BDNF leptin-resistance |
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