| 泸县沼水蛙16SrRNA基因克隆与系统发育分析 |
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| 引用本文： | 熊荣川,田应洲,李松,陈红.泸县沼水蛙16SrRNA基因克隆与系统发育分析[J].六盘水师范高等专科学校学报,2013(5):38-42. |
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| 作者姓名： | 熊荣川 田应洲 李松 陈红 |
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| 作者单位： | [1]六盘水师范学院生命科学系,贵州六盘水553001l大学生命科学学院,成都610064；3中国科学院成都生物研究所,成都610041；3中国科学院大学,北京100049 [2]四川大学生命科学学院,成都610064 [3]中科学院成都生物研究所,成都610041 [4]中国科学院大学,北京100049 |
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| 基金项目： | 贵州省科学技术基金项目（黔科合J字LKLS[2013]06号）;贵州省教育厅自然科学基金项目（黔教合ky字（2012）064;六盘水市科技局基金项目（2010）52020.2011-01-08-04. |
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| 摘 要： | 对采于四川省泸县地区的沼水蛙（Hylaranaguentheri）线粒体16SrRNA基因进行扩增测序。经3对扩增引物分别扩增及测序获得长度为550bp、300bp、1000bp核苷酸序列，拼接后序列长度1601bp。核苷酸相似性分析表明，序列与Genbank公布的福建的沼水蛙线粒体16SrRNA基因（KF185060）核苷酸序列相似性最高（99．8％）；与越南地区沼水蛙的16S序列相似性也较高（99％～99．7％），无明显种间差异，从遗传上证明沼水蛙为广布物种。同时，与亲源关系较近的水蛙类代表物种构建的系统发育树显示。所有沼水蛙聚为一支系，然后与Ranalateralis互为姊妹群。本文同时也对水蛙类的系统发育关系做了初步讨论。
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| 关 键 词： | 沼水蛙 16SrRNA 克隆 系统发育进化树 |
Sequencing and Phylogenetic Analysis of the 16SrRNA gene of Hylarana guentheri from Luxian County in Southern China |
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| XIONG Rong-Chuan,TIAN Ying-zhou,LI Song,CHEN Hong.Sequencing and Phylogenetic Analysis of the 16SrRNA gene of Hylarana guentheri from Luxian County in Southern China[J].Journal of Liupanshui Teachers College,2013(5):38-42. |
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| Authors: | XIONG Rong-Chuan TIAN Ying-zhou LI Song CHEN Hong |
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| Institution: | 1.Department of Biology Science, Liupanshui Normal University, Liupanshui 553001, China; 2. College of Life Sciences, Sichuan University, Chengdu 610064, China; 3.Chengdu Insititute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; 4. Graduate University of Chinese Academy of Sciences, Beijing 100049, China) |
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| Abstract: | The present paper clones and sequences the mitochondrial 16SrRNA of Hylarana guentheri from Luxian county in Sichuan province. Three nucleotide sequences （550bp, 300bp, 1000bp） have been cloned and sequenced with 3 pairs of primer were assembled into a complete 1601bp contig. The result shows that the sequence was 99.8% identical to that of another H. guentheri sample from Fujian province （KF185060）, and 99% - 99.7% to the ones from Vietnam. This result proves H. guentheri to be a widespread species geneti- cally. Further, the phylogenetic analysis including available 16S sequences of Hylarana species shows that all H. guentheri sequences clustered together as the sister group to that of Rana lateralis. In addition, the phyloge- netical relationship among Hylarana species has been discussed. |
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| Keywords: | Hylarana guentheri 16SrRNA gene cloning phylogenetical analysis |
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