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来航鸡Mx基因14个外显子克隆及测序
引用本文:尹春光,曹鼎国,李福伟,王晓强,潘丽,李城.来航鸡Mx基因14个外显子克隆及测序[J].济宁师范专科学校学报,2010,31(3):51-54.
作者姓名:尹春光  曹鼎国  李福伟  王晓强  潘丽  李城
作者单位:尹春光,王晓强,YIN Chunguang,WANG Xiaoqiang(济宁学院生命科学与工程系,山东,曲阜,273155);曹鼎国,李福伟,CAO Dingguo,LI Fuwei(山东省农业科学院,济南,250023);潘丽,李城,PAN Li,LI Cheng(生命科学与工程系) 
基金项目:国家高技术研究发展计划项目(863计划项目,山东省高等学校科技计划项目,济宁学院教改项目 
摘    要:禽类的Mx蛋白具有抵抗禽流感病毒及水疱性口炎病毒(VSV)的功能,对禽类Mx蛋白基因进行研究具有重要意义。以白来航(White Loghorn,WLH)作为实验动物,获得Mx蛋白基因全长并对基因组结构进行分析并克隆测序获得了Mx基因所有外显子区(1-14exons),为研究Mx基因结构及多态性分析奠定了基础。

关 键 词:Mx基因  外显子  克隆

Cloning and Sequencing 14 Exons of Mx Gene from White Leghorn
YIN Chunguang,CAO Dingguo,LI Fuwei,WANG Xiaoqiang,PAN Li,LI Cheng.Cloning and Sequencing 14 Exons of Mx Gene from White Leghorn[J].Journal of Jining Teachers College,2010,31(3):51-54.
Authors:YIN Chunguang  CAO Dingguo  LI Fuwei  WANG Xiaoqiang  PAN Li  LI Cheng
Institution:1.Life Sciences and Engineering Department,Jining University Qufu 273155,China;2.Institute of Poultry Science,Shandong Academy of Agricultural Sciences,Jinan 250023,China)
Abstract:Avian Mx protein was discovered to have antiviral activity(avian influenza and vesicular stomatitis).Because birds are natural hosts of avian influenza,the study of chicken Mx protein and its encoding genes is of significance.In the present study,White leghorns(WLH) were selected to obtain the full length of Mx cDNA and the genome structure was also analysised.All of the 14 exons were obtained and senquenced.The research forms the basis for the genome structure and polymorphism of Mx gene.
Keywords:Mx gene  exon  cloning
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