| 一氧化氮对增强UV-B胁迫下螺旋藻抗氧化酶活性的影响 |
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| 引用本文： | 薛林贵,李师翁,张宝芹,常思静.一氧化氮对增强UV-B胁迫下螺旋藻抗氧化酶活性的影响[J].天水师范学院学报,2009,29(5):25-30. |
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| 作者姓名： | 薛林贵 李师翁 张宝芹 常思静 |
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| 作者单位： | 兰州交通大学,化学与生物工程学院,甘肃,兰州,730070 |
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| 基金项目： | 国家自然科学基金项目"青海湖水生态系统中蓝藻对增强UV-B胁迫的响应" |
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| 摘 要： | 为了探讨一氧化氮对增强的UV-B胁迫下螺旋藻生理代谢的影响，通过测定细胞内蛋白质含量及抗氧化酶活性的变化证实了0．5mmol／L的一氧化氮（Nitric oxide，NO）供体硝普钠（Sodium nitroprusside，SNP）对增强UV-B胁迫下的螺旋藻（Spirulina platensis 794）细胞内蛋白质代谢及抗氧化系统产生明显影响．实验结果显示，NO能够显著诱导增强的UV-B胁迫下螺旋藻细胞内蛋白质含量的增加．促进正常生长条件下螺旋藻细胞内抗氧化酶活性的提高．说明NO对增强UV-B胁迫下的螺旋藻794细胞有保护作用．可以减轻UV-B胁迫对螺旋藻细胞引起的氧化损伤，这为进一步探讨NO信号及其与其他信号分子之间相互作用、相互关联来调节细胞的生理生化过程，以减缓UV-B胁迫下的氧化损伤机理提供了科学依据．
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| 关 键 词： | 螺旋藻 UV-B胁迫 一氧化氮 蛋白质 抗氧化酶 |
Effects of Nitric Oxide on the Antioxidant Enzyme Activity of Spirulina by Enhanced Ultraviolet-B Stress |
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| Xue Lingui,Li Shiweng,Zhang Baoqin,Chang Sijing.Effects of Nitric Oxide on the Antioxidant Enzyme Activity of Spirulina by Enhanced Ultraviolet-B Stress[J].Journal of Tianshui Normal University,2009,29(5):25-30. |
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| Authors: | Xue Lingui Li Shiweng Zhang Baoqin Chang Sijing |
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| Institution: | (School of Chemistry and Bioengineering, Lanzhou Jiaotong University, Lanzhou Gansu 730030, China) |
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| Abstract: | To study the effects of NO on the physiological metabolism of spirulina by enhanced UV-B stressed, the changes of protein content and the antioxidant enzyme activity were investigated under enhanced UV-B radiation and its combination with different chemical treatment. The changes of protein content and the antioxidant enzyme activity confirmed that 0.5mmol/L sodium nitroprusside （SNP）, a donor of nitric oxide （NO）, could make markedly effects on the protein metabolism and the activities of antioxidant systems of Spirulina platensis 794 caused by enhanced ultraviolet-B. Further resuhs proved that NO significan activities. These results suggest that NO has protective tly increased the content of protein and the antioxidant enzyme effect and can strongly alleviate the oxidant damage caused by UV-B stress in S. platensis 794 ceils. Reported the effect of NO on the regulating ability of oxidant damage of S. platensis induced by enhanced UV-B. interaction and inter-correlation of signal Therefore, further investigations will be necessary to inquire into the molecules and the mechanism in spirulina under enhanced UV-B stress by use of these results. |
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| Keywords: | spirulina ultraviolet-B stress nitric oxide protein antioxidant enzyme |
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