| 高住低训抑制大鼠骨骼肌mTOR蛋白表达 |
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| 引用本文： | 贺道远,曾凡星,王伟明.高住低训抑制大鼠骨骼肌mTOR蛋白表达[J].北京体育大学学报,2008,31(8). |
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| 作者姓名： | 贺道远 曾凡星 王伟明 |
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| 作者单位： | 1. 三峡大学体育学院,湖北,宜昌,443002
2. 北京体育大学,北京,100084 |
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| 基金项目： | 国家自然科学基金
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三峡大学科学基金
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北京体育大学博士论文基金 |
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| 摘 要： | 目的:探讨运动和低氧影响骨骼肌生长的分子调控机制。方法:SD大鼠分为6组:1)28 d组,包括对照组、常氧运动组、低氧暴露组、高住低训组;2)复氧7 d组,包括低氧暴露复氧7 d组、高住低训复氧7 d组,每组6只。常氧运动组进行4周的跑台运动。低氧暴露组白天与对照组在常氧下生活,晚上在氧浓度13.6%低氧舱内低氧暴露12 h;复氧7 d组进行低氧暴露后复氧7 d。高住低训组每天运动后1 h进行低氧暴露。实验后取腓肠肌称重,采用western blot测定mTOR蛋白表达。结果:1)28 d常氧运动后骨骼肌mTOR蛋白表达明显上降(p<0.05);2)28 d高住低训后骨骼肌mTOR蛋白表达明显下降(p<0.05),复氧7 d后显著回升(p<0.05)。结论:运动和低氧调节骨骼肌mTOR蛋白表达,提示高住低训抑制骨骼肌生长可能与运动、低氧调控骨骼肌mTOR信号并影响蛋白翻译过程有关。
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| 关 键 词： | 低氧 高住低训 翻译调控 哺乳动物雷帕霉素靶蛋白(mTOR) |
On the Living-High-Training-Low Restraining mTOR Protein Expression of Skeletal Muscle in Rats |
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| HE Dao-yuan,XENG Fan-xing,WANG Wei-ming.On the Living-High-Training-Low Restraining mTOR Protein Expression of Skeletal Muscle in Rats[J].Journal of Beijing Sport University,2008,31(8). |
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| Authors: | HE Dao-yuan XENG Fan-xing WANG Wei-ming |
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| Institution: | HE Dao-yuan1,ZENG Fan-xing2,WANG Wei-ming1 |
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| Abstract: | Objective: to research the effect of exercise and hypoxia on skeletal muscle growth and its mechanism of molecular regulation.Methods: SD rats were divided into 6 groups:(1) 28-days groups,included control group,normoxic exercise group,hypoxic exposure group,living high training low(HiLo) group.(2) 7days reoxygen groups,included 7 days of reoxygen groups after hypoxic exposure,7 days of reoxygen groups after HiLo.Normoxic exercise group were carried out treadmill running for 4 weeks.Hypoxic exposure group were treated with 13.6% concentrations of oxygen for 12 h/day under normobaric conditions in hypoxic chamber.7 days of reoxygen groups were treated with 7 days of reoxygen after hypoxic exposure.HiLo group were exposed to hypoxia following 1 hour after exercise.After gastrocnemius muscle weighing,mTOR expression in gastrocnemius muscle were tested with the method of western blot.Results: 1) After exercise for 28 days, mTOR protein expression in muscle increased significantly(p<0.05);2) After living high training low for 28 days,mTOR protein expression in muscle decreased significantly(p<0.05),but increased significantly after 7 days reoxygen(p<0.05).Conclusions: exercise and hypoxia regulate mTOR signal.HiLo repress muscle growth,and its mechanism may be the way of regulation of mTOR signal and protein translation in skeletal muscle by exercise and hypoxia. |
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| Keywords: | hypoxia living-high-training-low(HiLo) translation regulation mammalian target of rapamycin(mTOR) |
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