| 柏木属植物过氧化物酶同工酶的研究 |
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| 引用本文： | 江洪,王琳.柏木属植物过氧化物酶同工酶的研究[J].中国科学院研究生院学报,1986,24(4):253-259. |
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| 作者姓名： | 江洪 王琳 |
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| 作者单位： | 1(四川省林业学校，灌县) 2(四川省绵阳地区林业科学研究所，绵阳) |
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| 摘 要： | 用聚丙烯酰胺凝胶电泳分析了柏木属9个种的过氧化物酶同工酶。结果表明：属内种
间具有明显的酶谱差异，每个种都有其特征酶谱。并用排序的方法对柏木属9个种酶谱的相
似程度进行了研究，据酶谱的排序结果把9个种分为5个类群。其中：巨柏、西藏柏木、干香
柏、岷江柏木和剑阁柏木为一类群; 地中海柏木、柏木、墨西哥柏木和绿干柏各为一类群，从分
子水平上论证了前人对于柏木属各种间亲缘关系的论述。研究结果还表明，柏木属植物近缘
种间过氧化物酶同工酶谱相似程度的替代与近缘种间的地理替代表现出强烈的映射关系，这
种关系可以用来揭示植物地理替代的规律。柏木属内种间平均酶谱距离为0.75，推测其地质
发生年代为侏罗纪。本文还发现用排序的方法研究同工酶谱可以达到定量比较的水平，在实践中有较多的优越性。
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| 关 键 词： | 柏木属 同工酶谱 亲缘关系 |
The Peroxidase Isoenzymes of Cupressus Linn. |
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| Jiang Hong,Wang Ling.The Peroxidase Isoenzymes of Cupressus Linn.[J].Journal of the Graduate School of the Chinese Academy of Sciences,1986,24(4):253-259. |
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| Authors: | Jiang Hong Wang Ling |
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| Institution: | 1(Forestry School of Sichuan, Guan Xian)2
(Institute of Forest, Sichuan Mianyang, Mianyang) |
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| Abstract: | By means of disc polyacrylamide gel electrophoresis, peroxidase isoenzymes
of 9 species of Cupressus Linn, were analysed. The interspecific zymogramatic dif-
ferences are obvious. Each species possesses its specific zymogram, distinguishable form
the others. According to the zymogramatic similarity among these species calculated
with the method of polar ordination, they may be grouped into 5 groups, which may be
grouped in turn into two categories. C. gigantea Cheng et L. K. Fu, C. torulosa D.
Don, C. duclouxiana Hichel, C. chengiana S. Y. Hu, and C. jiangeensis N. Chao. fall
into one category. The second category contains C. sempervirens, C. arizonica Greene,
C. lusitanica and C. funebris Endl. The experimental result about peroxidase com-
firms the interspecific relationships of Cupressus suggested by the previous works. A
positive relation is found between the peroxidases isoenzyme zymogramatic similar de-
gree and the geographical vicarism of the related species, which has been used for ana-
lysing the regularity of phytogeographical vicarism. The mean zymogram distance of
Cupressus Linn. among species is 0.75, from which it is inferred that the divergency
took place the Jurassic. The advantage of polar ordination method in the study of iso-enzyme zymogram is discussed. |
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| Keywords: | Cupressus Linn Isoenzyme zymogram consanguinity |
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