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杉木CAD片段为信息探针,利用序列拼接方法获得了738bp的cDNA序列.利用MEGA4.0软件对不同进化层次的代表植物相应CAD基因进行比对,构建系统进化树.利用生物信息学方法分析了杉木CAD基因的结构与功能,其中包括核苷酸序列的组成、ORF识别、CpG岛、限制性酶切位点分析、卷曲螺旋、亲水性、糖基位点及二级结构.结果表明:杉木与铁杉的CAD基因具有99.00%的同源性;该序列在88~737bp片段上有一个开放性阅读框,该序列编码的是一个碱性蛋白,亲水性较强,疏水性较弱,信号肽序列为第1位~第11位,二级结构以α-螺旋(27.50%)与不规则盘绕(41.50%)为主,延伸链(22.50%)也是该蛋白的重要结构元件,β转角区域仅为8.50%. 相似文献
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固有无序蛋白是一类具有柔性结构的蛋白质,许多情况下通过与其它物质相互作用形成稳定结构来发挥重要生物功能.本文分别利用基于序列特征和结构特征的蛋白质结合位点预测程序对固有无序蛋白有序区和无序区与核酸分子的结合位点进行了预测分析.结果发现,基于结构特征的结合位点预测方法整体上要优于基于序列特征的预测方法,表明无序区尽管缺少稳定空间结构,结构特征依然在固有无序蛋白结合位点中发挥了重要作用.因此,可以为今后固有无序蛋白序列特征及结构特征的提取提供可靠依据. 相似文献
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一、主题活动“花生”的观察实录(一)活动目标1.认识花生,初步懂得花生的生长习性、结构特点。2.学习比较不同大小的花生、数花生果和花生仁。3.学习区分干、湿的花生,生、熟的花生。4.小肌肉的训练:动手洗花生,剥花生。(二)活动准备若干个小篮子、2个大盆;花生2斤;电饭堡等。(三)活动过程1.引入课题,激发兴趣(1)小朋友讨论:花生是什么样的?把自己所知道的情况告诉老师和同伴们。(2)提出主题,明确目的师:今天,我们来认识花生,还要一起煮花生,吃花生,好不好?2.了解花生的生长习性(1)先请幼儿猜想花生生长在哪里的。(2)通过亲自体验,寻找结果… 相似文献
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花生肽是花生蛋白质经过控制性的水解、精制以后得到的一类活性肽。本文对通过Alcalase碱性蛋白、Protamex复合蛋白酶和Neutrase中性蛋白酶水解花生分离蛋白制备的花生肽的体外抗氧化活性进行研究。采用亚油酸-硫氰酸钾方法、脱氧核糖方法和邻苯三酚方法测定花生肽的抗亚油酸过氧化能力、清除羟自由基能力和清除超氧阴离子的能力,从而对花生肽的体外抗氧化活性进行评价。结果表明用Alcalase碱性蛋白酶水解得到得花生肽抗氧化活性最强。 相似文献
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黄术 《广东技术师范学院学报》2010,31(6):7-10
根据Dedekind和S(h,p)的定义和性质,研究了某类二阶递归序列序列{An}的Dedekind和,得到了关于和式∑n=1^m S(An,An+1)的估计结果.从而把关于Fibonacci序列,Lucas序列及Pell序列的Dedekind和的有关估计结果推广到一般情况. 相似文献
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《赣南师范学院学报》2021,(3):125-129
为探讨高感溃疡病‘纽荷尔’脐橙CsLOB1基因序列特征及其响应柑橘溃疡菌侵染表达特点,利用同源序列法从‘纽荷尔’脐橙叶片中克隆得到CsLOB1基因CDS全长并进行序列分析.实时荧光定量法检测‘纽荷尔’脐橙叶片接种溃疡菌后CsLOB1基因表达变化.结果表明,CsLOB1基因CDS序列全长714 bp,编码237个氨基酸.理化性质预测分析表明CsLOB1蛋白为亲水性蛋白质;保守域预测表明,CsLOB1蛋白含有LBD基因家族LOB保守结构域.系统进化树分析表明,‘纽荷尔’脐橙与克里曼丁橘CsLOB1蛋白同源性高于其它非柑橘类植物.实时荧光定量PCR分析显示,柑橘溃疡菌接种后,‘纽荷尔’脐橙叶片CsLOB1基因表达显著上调.采用gateway克隆技术进一步构建CsLOB1基因RNAi干涉载体,为柑橘抗溃疡病种质的创建建立基础. 相似文献
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王双怀 《历史教学(高校版)》1998,(3)
花生又名番豆、地豆、长生果、落花生,原产于南美洲的巴西和秘鲁等地.从植物学的角度来讲,花生属豆科一年生草本植物.这种植物含有大量的脂肪和蛋白质,具有很高的营养价值,不仅是重要的副食品,而且是重要的油料作物.现在一般将花生作为经济作物看待,但明代花生主要是充当食物使用的.所以这里把花生放在粮食作物中. 相似文献
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醋浸后花生脂肪酸的变化及其营养学意义 总被引:1,自引:0,他引:1
我国民间有关于服用醋花生预防疾病的说法,文章在通过用气相色谱法测定了醋浸后花生脂肪酸变化的基础上,探讨醋花生作为保健食品的作用机理。认为,在醋浸过程中,醋花生的饱和脂肪酸相对含量下降,多不饱和脂肪酸特别是亚油酸相对含量显著升高,从而说明在防治心血管疾病方面醋花生有更显著的功效,降血脂的作用更突出。 相似文献
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申满秀 《遵义师范学院学报》2001,3(3):97-99
作为世界三大宗教之一的伊斯兰教,其产生时间晚,且发展迅速,尤其在当今世界更显现其强劲的发展态势.本文通过伊斯兰教的产生、发展状况及社会现实剖析伊斯兰教在阿拉伯国家建立、发展中的凝聚作用;各种教派矛盾斗争中的调和作用;阿拉伯国家社会前进探索道路上的激励作用.正是这些独特作用,才使阿拉伯国家的伊斯兰教充满活力,经久不衰. 相似文献
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用40%达科宁胶悬剂500、750、1000倍液,在常年花生叶斑病发生较重的地块上于花生盛花期,喷施花生茎叶.试验表明,用40%达科宁胶悬剂500-750倍液防治花生叶斑病效果好,促进了花生生长,提高了花生产量。 相似文献
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《Australian Journal of Learning Difficulties》2013,18(2):39-41
Spelling: Approaches to teaching and assessment Peter Westwood 1999 Melbourne, Australia Australian Council of Educational Research $19.95 (plus GST) A4 size, 80 pages. Handwriting of the twentieth century Rosemary Sassoon 1999 London and NewYork. Routledge, 11, New Fetter Lane, London, EC4P 4EE $49.95 Paperback, size 245 × 175 mm, 208 pp. The Macadamia Series of Reading Boob Dykes B, Swallow G and Ward S 1996 Kalbar, Australia Nutshell Products, PO Box 6 Kalbar Complete Set: $139.75 Individual Sets: $29.95 each 5 sets of graded readers; 10 in each set: Gumnut, Peanut, Walnut, Coconut, and Bunya Nut. South Asian Children and Adolescents in Britain Lau A. 2000 London Whurr Publishers MacLennan and Petty $74.00 Educational Therapy in Clinic and Classroom Muriel Barrett & Ved Varma 1996 London Whurr Publishers Maclennan & Petty, Sydney $74–00 Power Tools for Positive Living. Positive optimism for young people Ian McLean & Elizabeth Redman 1999 Mitcham, Victoria HiPerform Learning SPELD Victoria $19.95 Size 200mm x 170mm, 105 pages, paperback, multi‐coloured cover, illustrated with charming, black and white drawings. 相似文献
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GM1 stabilizes expression of NMDA receptor subunit 1 in the ischemic hemisphere of MCAo/reperfusion rat 总被引:3,自引:0,他引:3
Liu JR Ding MP Wei EQ Luo JH Song Y Huang JZ Ge QF Hu H Zhu LJ 《Journal of Zhejiang University. Science. B》2005,6(4):254-258
INTRODUCTION GM1 ganglioside (GM1) is the main kind ofgangliosides in mammalia, and most abundant inbrain tissue (Duchemin et al., 2002). It was reportedthat GM1 could protect cerebral ischemia in vivo andin vitro, one protective mechanism of which is thatGM1 could reduce neural injury induced by toxicityof excitatory amino acid via N-methyl-D-aspartate receptor (NMDAR) (Kharlamov et al., 1993; Simon et al., 1993; Garofalo and Cue… 相似文献
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Jian Huang Feng-lei Du Yuan Yao Qun Wan Xiao-song Wang Fei-yan Chen 《Journal of Zhejiang University. Science. B》2015,16(8):661-671
Distance effect has been regarded as the best established marker of basic numerical magnitude processes and is related to individual mathematical abilities. A larger behavioral distance effect is suggested to be concomitant with lower mathematical achievement in children. However, the relationship between distance effect and superior mathematical abilities is unclear. One could get superior mathematical abilities by acquiring the skill of abacus-based mental calculation (AMC), which can be used to solve calculation problems with exceptional speed and high accuracy. In the current study, we explore the relationship between distance effect and superior mathematical abilities by examining whether and how the AMC training modifies numerical magnitude processing. Thus, mathematical competencies were tested in 18 abacus-trained children (who accepted the AMC training) and 18 non-trained children. Electroencephalography (EEG) waveforms were recorded when these children executed numerical comparison tasks in both Arabic digit and dot array forms. We found that: (a) the abacus-trained group had superior mathematical abilities than their peers; (b) distance effects were found both in behavioral results and on EEG waveforms; (c) the distance effect size of the average amplitude on the late negative-going component was different between groups in the digit task, with a larger effect size for abacus-trained children; (d) both the behavioral and EEG distance effects were modulated by the notation. These results revealed that the neural substrates of magnitude processing were modified by AMC training, and suggested that the mechanism of the representation of numerical magnitude for children with superior mathematical abilities was different from their peers. In addition, the results provide evidence for a view of non-abstract numerical representation. 相似文献
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Basic fibroblast growth factor alleviates brain injury following global ischemia reperfusion in rabbits 总被引:9,自引:0,他引:9
Zhang M Ma YF Gan JX Jiang GY Xu SX Tao XL Hong A Li JK 《Journal of Zhejiang University. Science. B》2005,6(7):637-643
The aim of this study was to explore the protective effect of basic fibroblast growth factor (bFGF) on brain injury following global ischemia reperfusion and its mechanisms. Brain injury following global ischemia was induced by four vessels occlusion and systemic hypotension. Twenty-four rabbits were randomized into three groups: group A, only dissection of vessels; group B, intravenous infusion of normal saline after reperfusion for 6 h; group C, 30 μg/kg bFGF injected intravenously at the onset of reperfusion, then infused with 10 μg/(kg·h) for 6 h. Serum neuron specific enolase (NSE), S-100B, tumor necrosis factor-α(TNF-α), interleukin-1 (IL-1), interleukin-8 (IL-8) were measured before ischemia, 30 min after ischemia, 0.5, 1, 3, 6 h after reperfusion. Brain water content was determined and cerebral histopathological damages were compared. NSE and S-100B were increased 1 h after reperfusion and reached their peaks 6 h after reperfusion, but were much higher in group B than those in group C 3, 6 h after reperfusion. In groups B and C, TNF-a was increased after ischemia and IL-1 and IL-8 were increased significantly 0.5 h after reperfusion, then reached their peaks 6 h, 3 h, 6 h after reperfusion respectively. TNF-a and IL-8 at the time points of 1 h and 3 h and IL-1 at 3 h and 6 h in group C were correspondingly lower than those in group B. These indices in group A were nearly unchanged. There were less severe cerebral histopathological damages in group C compared with group B, but no difference in brain water content. It could be concluded that bFGF alleviates brain injury following global ischemia and reperfusion by down-regulating expression of inflammatory factors and inhibiting their activities. 相似文献
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闫林 《石家庄师范专科学校学报》2013,(3):96-99
通过实验研究运动员午睡的影响作用和睡眠惯性的持续时间.结果显示,1h午睡后,运动员的大脑机能状态、神经-肌肉兴奋性和心境状态等指标在醒后0.5h仍处于较低水平。而在醒后1h却显著提高,且超过睡前基线水平;而睡眠惯性的持续时间要长于0.5h,在1h左右基本消退. 相似文献
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Formalist conceptions of aesthetic goals began to give way from the 17th century on to rationalist accounts of experience. Beauty as described as essential by Renaissance thinkers was reconceived as just one possibility, with the sublime in Burke's account as another. In the early 20th century Clive Bell again asserted a formalist position with his argument for significant form. The weakness of the formality position, and the need for a richer, more contextual account of aesthetic goals and experience is argued with reference to the idea of thick and thin concepts as applied by Bernard Williams to ethics, Wittgenstein's attention to the way in which language is used in a specific context, and Scruton's emphasis on an informed conception of the object of aesthetic attention. Beauty, in Alberti's procedural account if not in his version of the Platonic ideal, can be seen to continue to have critical value. The idea of character, however, offers a broader range of possibilities for experience and of goals for design. 相似文献
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萘乙酸(NAA)是植物生长发育过程中一类重要的调节激素,在植物生长发育中起着重要作用.以甘草种子为材料,利用0.01 mg·L-1、0.1 mg·L-1和1 mg·L-1三种不同浓度的萘乙酸溶液浸种分别处理不同时间.结果表明:萘乙酸对甘草种子活力起重要的作用,适当的萘乙酸的摄入能使甘草种子的活力有明显提高.不同萘乙酸溶液处理下的甘草种子活力差异比较大,0.01 mg·L-1萘乙酸溶液处理下的种子活力比0.1 mg·L-1和1 mg·L-1的高;从处理时间方面,处理36 h后的种子活力最高,且差异比较明显;并且在所有实验组中,以0.01 mg·L-1萘乙酸溶液处理36 h的甘草种子活力为最高. 相似文献