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采用梯度淋洗离子色谱法对碱金属、碱土金属及其铵类进行分析，该方法采用ＣＧ３分离柱，０．３ｍｍｏｌ／ＬＨＣｌ为淋洗剂，４ｍｉｎ后改用３．０ｍｍｏｌ／Ｌ盐酸间苯二胺和５．０ｍｍｏ１／ＬＨＣｌ作淋洗剂（流速２．０ｍｌ／ｍｉｎ）采用微膜抑制柱，再生液为５０ｍｍｏｌ／ＬＫＯＨ（流速２．０ｍｌ／ｍｉｎ），用电导检测法分别检测Ｌｉ￣＋、Ｎａ￣＋、ＮＨ、Ｋ￣＋和Ｍｇ￣（２＋）、Ｃａ￣（２＋）、Ｓｒ￣（２＋）、Ｂａ￣（２＋）．对分析条件进行了探讨． 相似文献
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目的：探讨西藏拉萨地区乙型肝炎病毒（ＨＢＶ）血清免疫学标记的组合模式。方法 共２２４８例ＨＢＶ血清样本，均以免疫酶联技术（ＥＬＩＳＡ）法检测５项指标。以聚合莲反应（ＰＣＲ）检测ＨＢＶ ＤＮＡ。为表述方便，设定血清ＨＢＶ免疫学标记检测项目第１－５项的排列顺序为（１）乙肝表面抗原（ＨＢｓＡｇ），（２）乙肝表面抗体（抗－ＨＢｓ），（３）乙肝ｅ抗原（ＨＢｓＡｇ），（４）乙肝ｅ抗体（抗－ＨＢｅ），（５）乙肝 相似文献
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采用聚丙烯酰胺凝胺电泳法对９２头德令哈地区的柴达木黄牛血清淀粉酶同工酶进行了分析。结果发现：（１）柴达木黄牛的血清淀粉酶有ＡＭＹ１,ＡＭＹ２和ＡＭＹ３三种同工酶;（２）ＡＭＹ１同工酶因存在ＡＭＹ１ＢＢ,ＡＭＹ１ＢＣ和ＡＭＹ１ＣＣ三种基因型而表现出多态性;（３）公健牛组与母牛组ＡＭＹ１基因频率和基因型分布没有显著差异。 相似文献
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ＣＨＡＲＡＣＴＥＲＩＳＴＩＣＳＯＦＴＨＥＦＲＡＣＴＡＬＳＡＰＰＥＡＲＥＤＯＮＡＬＵＭＩＮＩＵＭＦＯＩＬＩＲＲＡＤＩＡＴＥＤＢＹＩＮＴＥＮＳＥＲＥＬＡＴＩＶＩＳＴＩＣＥＬＥＣＴＲＯＮＢＥＡＭ￥ＧａｏＨｕａｉ－ｌｉｎ；ＰａｎＹｕ－ｌｉ（ＣｈｉｎａＩｎｓｔ... 相似文献
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氨基酸清除活性氧自由基作用的研究 总被引：22，自引：0，他引：22
分别采用Ｘ－ＸＯＤ－Ｌｕｍｉｎｏｌ的Ｏ２＾－产生模型和Ｖｃ－Ｃｕ＾２＋－Ｈ２Ｏ２－酵母多糖的ＯＨ产生体系，以还原型谷胱甙肽（ＧＳＨ）作为参照，用化学发光法研究了１９在酸对活性氧自由基的清除能力，证实了氨基酸普遍具有生物抗氧化活性，其抗Ｏ２＾－的有效浓度范围在１０＾－２￣１０＾－４ｍｏｌ／Ｌ，比ＧＳＨ高出二个数量级。 相似文献
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用反相纸层析法在ＨＣ１体系中考察了Ｎ,Ｎ－二丁基甘氨酸对Ｌａ＾３＋、Ｓｍ＾３＋等４２种离子的萃取能力。实验表明,此萃取剂对Ｎｄ＾３＋、Ｇｄ＾３＋等１８种离子具有较强的萃取能力。 相似文献
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ＭＥＡＳＵＲＥＭＥＮＴＯＦＳＯＤＡＣＴＩＶＩＴＹＢＹ~（１９）ＦＮＵＣＬＥＡＲＭＡＧＮＥＴＩＣＲＥＳＯＮＡＮＣＥ（ＮＭＲ）ＲＥＬＡＸＡＴＩＯＮＲＡＴＥＺｈａｏＢａｏ－Ｌｕ；ＣｈｅｎＹｕｎ－ＺｕｎａｎｄＸｉｎＷｅｎ－Ｊｕａｎ（ＩｎｓｔｉｔｕｔｅｏｆＢｉ?.. 相似文献
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Amit Kumar Mani Tiwari Abbas Ali Mahdi Fatima Zahra Sudarshna Sharma Mahendra Pal Singh Negi 《Indian journal of clinical biochemistry : IJCB》2012,27(3):246-252
To correlate blood lead levels (BLLs) and oxidative stress parameters in pregnant anemic women. A total of 175 pregnant women were found suitable and included for this study. Following WHO criteria, 50 each were identified as non-anemic, mild anemic and moderate anemic and 25 were severe anemic. The age of all study subjects ranged from 24–41 years. At admission, BLLs and oxidative stress parameters were estimated as per standard protocols and subjected with ANOVA, Pearson correlation analysis and cluster analysis. Results showed significantly (p < 0.01) high BLLs, zinc protoporphyrin (ZPP), oxidized glutathione (GSSG), lipid peroxide (LPO) levels while low delta aminolevulinic acid dehydratase (δ-ALAD), iron (Fe), selenium (Se), zinc (Zn), haemoglobin (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), red blood cell (RBC) count, reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and total antioxidant capacity (TAC) in all groups of anemic pregnant women as compared with non anemic pregnant women. In all groups of pregnant women, BLLs showed significant (p < 0.01) and direct association with ZPP, GSSG and LPO while inverse relation with δ-ALAD, Fe, Se, Zn, Hb, Hct, MCV, MCH, MCHC, RBC, GSH, SOD, CAT and TAC. Study concluded that low BLLs perturb oxidant-antioxidant balance and negatively affected hematological parameters which may eventually Pb to Fe deficiency anemia during pregnancy. 相似文献
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We have previously reported that microthrottle pumps (MTPs) display the capacity to pump solid phase suspensions such as polystyrene beads which prove challenging to most microfluidic pumps. In this paper we report employing a linear microthrottle pump (LMTP) to pump whole, undiluted, anticoagulated, human venous blood at 200 μl min(-1) with minimal erythrocyte lysis and no observed pump blockage. LMTPs are particularly well suited to particle suspension transport by virtue of their relatively unimpeded internal flow-path. Micropumping of whole blood represents a rigorous real-world test of cell suspension transport given blood's high cell content by volume and erythrocytes' relative fragility. A modification of the standard Drabkin method and its validation to spectrophotometrically quantify low levels of erythrocyte lysis by hemoglobin release is also reported. Erythrocyte lysis rates resulting from transport via LMTP are determined to be below one cell in 500 at a pumping rate of 102 μl min(-1). 相似文献
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Pankaj Abrol Neeraj Sharma Harbans Lal 《Indian journal of clinical biochemistry : IJCB》1997,12(2):125-127
Serum vitamin E and cholesterol concentrations were measured in 50 children with protein energy malnutrition (PEM) and results
were compared with a group of 50 normal controls. Mean serum vitamin E concentration as well as vitamin E/cholesterol ratio
were found to be reduced in children with PEM while their mean serum cholesterol level was not significantly different from
the control group. Data suggest that the reduced levels of serum vitamin E in PEM may be a result of malnutrition per se. 相似文献
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Maryam J. Jalali Madhavi S. Phadke 《Indian journal of clinical biochemistry : IJCB》2011,26(4):407-412
Conditions like hypertension, atherosclerosis and diabetes are known to be the result of endothelial dysfunction which could
begin early in the life of an individual. The markers of endothelial dysfunction studied in the present work are plasma hemoglobin,
serum high sensitivity C-reactive protein (hsCRP) and plasma nitrite and nitrate. We studied the onset of endothelial dysfunction
with increase in age as well as in disease condition like newly detected hypertensives and of hypertensive diabetic patients.
The mean plasma hemoglobin and hsCRP values were found to increase with age. hsCRP and plasma hemoglobin levels were significantly
higher in patient groups as compared to aged-matched controls. On the other hand nitrate and nitrite was significantly higher
in patients who suffered from both diabetes and hypertension together and nitrate values were significantly lower in patients
who suffered from hypertensive alone. Plasma hemoglobin and hsCRP are associated with endothelial dysfunction and increases
both with age as well as in disease condition. 相似文献
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Amit Jain D. Puri M. M. A. Faridi 《Indian journal of clinical biochemistry : IJCB》1997,12(2):119-124
Double volume (170 ml/kg body weight) exchange transfusion was done in 52 term infants in the first week of life for neonatal
hyperbilirubinemia. The M:F ratio was 1.08:1 and 37 (71.1%) babies were of low birth weight. Causes of jaundice were hemolytic
in 46.2% and non-hemolytic in 41.3% cases; in 13.5% babies no cause of jaundice could be found. After exchange transfusion
a fall of 14.6% and 47.4% was observed in the hemoglobin and serum bilirubin levels respectively. There was significant (p=0.0414)
rise in the mean mid exchange and post-exchange serum sodium levels as compared to pre-exchange values and it was found to
be due to higher donor's serum sodium levels (p=0.007). There was no effect on the serum potassium levels during or after
ET.
In general serum calcium levels significantly increased at mid-exchange period (p=0.0029) but post-exchange levels were same
as pre-exchange. Donor's serum calcium level had no effect on the infant's serum calcium level (p=0.993). There was no change
in the serum phosphate and blood urea levels during and after exchange-transfusion. The plasma glucose was significantly raised
during and after ET and plasma glucose of the donors had significant effect on the infant's plasma glucose levels (p=0.043).
Similarly plasma osmolality also showed significant increase during and after ET which was due to the effect of donor's plasma
osmolality (p=0.007). 相似文献
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Y. S. Mahindrakar A. N. Suryakar R. D. Ankush R. V. Katkam K. M. Kumbhar 《Indian journal of clinical biochemistry : IJCB》2007,22(1):91-94
New findings on organization of blood cell cytoskeleton represent an exciting aspect of modem cell biology and hematology,
which is an interesting investigation to study diabetes. The present study was undertaken in 150 subjects. Out of these, 30
subjects were controls (Group I) and 30 were type-2 diabetics without any complication (Group II), while remaining 90 subjects
were type-2 diabetics with complication (Group III). We determined erythrocyte spectrin and hemoglobin glycosylation and also
estimated plasma lipid peroxide, nitric oxide and erythrocyte glutathione peroxidase activity to assess the status of oxidative
stress. There was a significant increase in spectrin (P<0.001) and hemoglobin (P<0.001) glycosylation in Group II and III
as compared to Group I and spectrin glycosylation was nearly three times more as compared to hemoglobin, whereas plasma levels
of lipid peroxide (P<0.001) as well as nitric oxide (P<0.001) were found to be significantly increased and GPx activity (P<0.001)
was significantly decreased in Group II and III as compared to Group I. However, it was also observed that spectrin (P>0.05)
and hemoglobin (P>0.05) glycosylation was not significantly different in Group II and III. In contrast, there was significant
rise in lipid peroxide (P<0.001), nitric oxide (P<0.001) and fall in GPx activity (P<0.001) in Group III when compared to
Group II. Increased erythrocyte protein glycosylation and oxidative stress is clearly evident from our study. However, to
understand the exact interplay between these two mechanisms, further studies are required. 相似文献
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Ajay Kumar Khanna Sujit Yadav Z. Ali N. C. Arya Anuradha Khanna 《Indian journal of clinical biochemistry : IJCB》1996,11(1):52-55
Serum Adenosine Deaminase (ADA) activity was studied in 20 control cases and 32 patients with carcinoma breast. In control group the ADA activity ranged from 13–94 IU/L with a mean of 43.75±21.55 IU/L while in patients of carcinoma breast it was 16–95 IU/L with a mean of 47.46±22.54 IU/L, but these values were not statistically different. The ADA activity was also compared with other parameters in patients of carcinoma breast but the difference was not statistically significant in the various parameters like duration of disease, menopausal status, tumor size, hemoglobin level, total leucocyte count, absolute lymphocyte count, total serum proteins, serum albumin, histological grade of tumor and lymphocytic infiltration. The only significant difference in ADA activity was when N1 status of lymph node was compared with N2 status of lymph node. 相似文献
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S. K. Pandey S. Pandey R. M. Mishra M. Indurkar 《Indian journal of clinical biochemistry : IJCB》2017,32(1):103-105
Normal iron levels are required to prevent thrombocytosis by inhibiting thrombopoiesis. Thrombocytosis is usually associated with a mild iron deficiency and is the result of a lack of inhibition of thrombopoiesis. Study participants were 430 iron deficiency anemia (IDA) patients. Ten (10) mL of venous blood were collected for the subjects. Ferritin analysis was done by ELISA method while Hemogram analysis was done by auto-analyzer. Factor V Leiden, PRTG20210A, and MTHFR C677T genotype analysis was performed by PCR–RFLP method. Among the patients, 9 were heterozygous (G>A) and 2 were homozygous (A>A) carrier of FV Leiden; while 20 were heterozygous (C>T) and 3 were homozygous (T>T) for MTHFR polymorphism. None of the patient was identified with PT mutation. Patients with thrombosis gene marker had lower hemoglobin, mean corpuscular volume, mean corpuscular haemoglobin levels, and mean corpuscular hemoglobin concentration than patients without thrombosis gene marker. Serum ferritin was elevated in subject with the absence of thrombosis gene markers. Our data suggest a high impact of inherited hypercoagulability risk factors in the pathogenesis of IDA and its complications. 相似文献
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Nilima N. Dongre Adinath N. Suryakar Arun J. Patil Jeevan G. Ambekar Dileep B. Rathi 《Indian journal of clinical biochemistry : IJCB》2011,26(4):400-406
The purpose of this study was to find out the effect of lead exposure on systolic and diastolic blood pressure, heme biosynthesis
related and hematological parameters of automobile workers. For this study 30 automobile workers were selected and compared
with 30 age matched healthy control subjects. Significantly increased blood lead (364%, P < 0.001) and urinary lead (176%, P < 0.001) levels were observed in automobile workers (study group) as compared to controls. Systolic blood pressure (5.32%,
P < 0.05) and diastolic blood pressure (5.87%, P < 0.05) were significantly increased in the automobile workers as compared to controls. The significantly decreased non-activated
erythrocyte δ-aminolevulinic acid dehydratase (δ-ALAD) (−18.51%, P < 0.01) and activated δ-ALAD (−13.29%, P < 0.05) levels were observed in automobile workers as compared to normal healthy control subjects. But the ratio of activated/non-activated
δ-ALAD was significantly increased (43.83%, P < 0.001) in automobile workers as compared to controls. Excretions of δ-aminolevulinic acid (83.78%, P < 0.001) and porphobilinogen (37%, P < 0.001) in urine were significantly increased in the study group as compared to the controls. In automobile workers heamoglobin
(−11.51%, P < 0.001), hematocrit (−4.06%, P < 0.05), mean corpuscle volume (−3.34%, P < 0.05), mean corpuscle hemoglobin (−5.66%, P < 0.01), mean corpuscle hemoglobin concentration (−7.67%, P < 0.001), red blood cell count (−14.6%, P < 0.001) were significantly decreased and total white blood cell count (11.44%, P < 0.05) increased as compared to the controls. The results of this study clearly indicate that the absorption of lead is
more in automobile workers and it affects on blood pressure, heme biosynthesis and hematological parameters observed in this
study group. 相似文献
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Bhalla Kapil Shukla Rimi V. P. Gupta S. Pugazhenthi K. M. Prabhu 《Indian journal of clinical biochemistry : IJCB》1995,10(2):57-61
In the present study, 58 diabetic patients (type II) comprising uncomplicated (27) and complicated (31) cases along with 38 healthy matched controls were investigated for glycosylated hemoglobin (HbA1), glycosylated serum proteins (GSP) and serum lipid profile viz. triacylglycerol, total cholesterol, HDL-cholesterol (HDL-C), VLDL-C, LDL-C and their ratios. HbA1 and GSP were significantly elevated in diabetic group as a whole when compared with controls (p<0.01), but comparison of mean values of complicated and uncomplicated group revealed (though higher in complicated) that the difference between two groups was not statistically significant (p>0.05). Follow-up studies showed that while GSP levels reflect variations in blood glucose over a shorter period of 15 days, those of HbA1 do so for about 3 months. HbA1 correlated with fasting blood glucose (p<0.01), whereas GSP showed correlation with both fasting (p<0.01) and postprandial (p<0.05) blood glucose. Hypercholesterolemia and hypertriacylglycerolemia were observed (p<0.01) in both the groups. However no significant change was seen in HDL-C (p>0.05) even after accounting for sex in both the groups. Interestingly TC:HDL-C was elevated significantly (p<0.01) in the above groups. These findings seem to suggest that at the levels of hyperglycemia, hypercholesterolemia and hypertriacylglycerolemia observed, some patients are prone to develop complications earlier. 相似文献