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Thangarasu Rajakumar Pachaiappan Pugalendhi Subbaiyan Thilagavathi 《Indian journal of clinical biochemistry : IJCB》2018,33(2):171-177
The present study aimed to investigate the protective effect of allyl isothiocyanate (AITC) on glycoprotein components in 7,12-dimethylbenz(a)anthracene (DMBA) induced mammary carcinogenesis in female Sprague–Dawley rats. Mammary tumor was induced by a single dose of DMBA (25 mg/rat) injected subcutaneously near mammary gland. The levels of glycoprotein components such as hexose, hexosamine and sialic acid were analyzed colorimetrically in plasma, mammary and liver tissues. We observed an increased levels of glycoprotein components in plasma, mammary and liver tissues in cancer bearing rats. It was further confirmed by Periodic Acid Schiff staining in mammary and liver tissues. Upon oral administration of AITC to DMBA injected rats, the abnormal changes were reverted back to near normal levels and biochemical findings are supported by histological analysis. This could be due to the anti-neoplastic potential of AITC against DMBA-induced mammary carcinogenesis. The result shows that AITC has the potential to inhibit abnormal glycosylation that favors neoplastic transformation. 相似文献
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粒细胞集落刺激因子（Ｇ－ＧＳＦ）对中性粒细胞减少症的治疗有良好的作用,对于肿瘤治疗有重要意义．利用小鼠ＷＡＰ基因的调控序列指导人Ｇ－ＣＳＦ基因所构建的融合基因,注射小鼠受精卵,获得了乳腺表达Ｇ－ＣＳＦ的转基因小鼠,为大规模生产提供了资料和方法． 相似文献
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BackgroundCancer is a life-threatening disease that affects approximately 18 million individuals worldwide. Breast cancer is the most common female neoplasm globally with more than 276,480 new cases of invasive breast cancer expected to be diagnosed in women in the U.S. alone in 2020. Genetic and epigenetic factors play role in the carcinogenesis and progression of this disease. In this study, MCF-7 adenocarcinoma cells were transfected with CRISPR/Cas9 plasmid to either knock out CDK11 or to activate CDH1. Treated cells were allografted into the mammary glands of female rats (150–190 g, 6–8 weeks) to evaluate the capability of these cells to control cancer progression and metastasis.ResultsqPCR data revealed a significant downregulation of CDK11 and upregulation of CDH1. Cell cycle analysis and apoptosis assays indicated the knockout of CDK11 and simultaneous activation of CDH1 resulted in cell cycle arrest at G2/M phase and accumulation of cells at G2. Meanwhile, the percentage of cells that underwent late apoptosis increased in both genome editing hits. Histopathological sectioning data indicated that untransfected MCF-7 cells were capable of developing tumors in the mammary gland and initiation g angiogenesis. Transfected cells significantly restricted cancer cell infiltration/invasion by minimally localizing tumors and inhibiting angiogenesis.ConclusionsAlthough further investigation is needed, the present data indicate the potentiality of using CRISPR/Cas9-based therapy as a promising approach to treat breast cancer. Impact: these data indicate targeting cancer-related genes via any genome editing tool might represent a novel approach to combat cancer.How to cite: Al-Mulhim F, Alqosaibi AI, Al-Muhnna A, et al. CRISPR/Cas9-mediated activation of CDH1 suppresses metastasis of breast cancer in rats. Electron J Biotechnol 2021;53. https://doi.org/10.1016/j.ejbt.2021.06.002 相似文献
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Subbaiyan Thilagavathi Pachaiappan Pugalendhi Thangarasu Rajakumar Krishnamoorthy Vasudevan 《Indian journal of clinical biochemistry : IJCB》2018,33(4):387-396
Bisphenol-A (BPA) is a ubiquitous environmental chemical that produces adverse effect on reproduction system due to its potent estrogenic endocrine disruptive activity. The present study was aimed to investigate the monotonic dose effect of BPA on estrogen synthesis in female Sprague-Dawley rats. For this purpose, we administered three different doses of BPA (10, 50, 100 µg/kg bw/day) into rats and analyzed various biochemical, hormonal, molecular and histological parameters. 10 µg BPA treated rats showed significantly decreased levels of phase I detoxification agents (CYP450, Cyt-b5). Overexpression of eNOS with decreased expression of StAR and steroidogenic enzymes (CYP11A1, aromatase) indicate decreased production of estrogen. Increased levels of serum gonadotropins (FSH, LH) and decreased levels of estradiol suggest mimetic action of BPA and its feedback inhibition. Increased body weight, lipid profile status of 10 µg BPA treated rats and histological analysis of ovary and mammary tissue support the study. Overall, our results suggest that BPA exerts its estrogen mimetic effects in a monotonic manner. 相似文献
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S. Kaushik S. S. Trivedi A. Jain J. Bhattacharjee 《Indian journal of clinical biochemistry : IJCB》2002,17(2):68-73
Colostrum, the mammary secretion during first 2–4 days of lactation, provides all the essential components of nutrition and
passive immunity required by the newborn. Pregnancy induced hypertension (PIH), glucose intolerance and anaemia are common
medical complications observed during pregnancy in Indian women and their effects were studied on the composition of colostrum
collected within 24 hours of delivery from lactating women included in the study. PIH during pregnancy significantly decreased
colostrum IgA and total proteins, but showed a significant increase in K+ levels, where as women with glucose intolerance showed a significant decrease in total lipids and lactose and an increase
in Na+ levels in colostrum compared to normal controls. The group with anaemia also showed a significant decrease in colostrum IgA
and total protein levels when compared with the control group. Awareness about the changes that occur in the composition of
colostrum during complicated pregnancies can be an important and useful tool for preventive and protective paediatrics. 相似文献
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Stainsloss Isabella Sankaran Mirunalini Kannaiyan Pandiyan 《Indian journal of clinical biochemistry : IJCB》2018,33(4):397-405
The present study aimed to investigate the effect of 3,3′-diindolylmethane (DIM) on inflammatory markers, estrogen receptors (ER), progesterone receptors (PR), level of glycoprotein and the mast cell population in 7,12-dimethylbenz (a) anthracene (DMBA) 25 mg/kg b.wt. induced rat mammary carcinogenesis. After 8 weeks of tumor formation, rats had access to an oral administrated with DIM 10 mg/kg b.wt. and DIM@CS-NP 0.5 mg/kg body weight respectively for 8 weeks. The oral administration of DIM@CS-NP 0.5 mg/kg b.wt. suppressed the Cox-2, NF-κB and TNF-α protein expression on DMBA induced rats compared to DIM 10 mg/kg b.wt. The ER/PR levels were increased on DMBA induced rats, treated with DIM@CS-NP 0.5 mg/kg b.wt. reduced ER/PR level as well as glycoprotein and mast cell population than DIM 10 mg/kg b.wt. The result shows that, DIM@CS-NP 0.5 mg/kg b.wt. has the potentially inhibit abnormal levels of inflammatory markers, ER, PR, levels of glycoprotein and mast cell population compared to DIM 10 mg/kg b.wt. 相似文献
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办学特色是指大学在长期办学过程中积淀形成的、特有的、稳定的、区别于其他高校的独特优质风貌,以及在办学过程中形成的体现一所高校办学优势和特性的总和。地方高校要实现较好发展,根本出路在于特色办学,走特色化发展道路。地方高校办学特色培育是一项系统工程,要整体考虑,把握凝练、构建和路径三个环节,要以现有特色凝练提升为前提,整体特色体系构建为核心,特色培育路径选择为关键,真正实施"特色兴校"战略,推动地方高校健康发展。 相似文献
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PowerPoint是微软公司推出的一款功能强大、实用性较强的多媒体课件开发工具。因它简单易学、容易上手,已成为目前教师制作课件的首选工具。利用PowerPoint自身提供的动画设置及触发器功能,完成了多媒体课件设计中经常需要采用其它交互性较强的软件才能实现的功能：下拉菜单的设计。 相似文献
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英语写作能力是一种高层次的交际能力,也是一种生存能力,外语教育者必须重视写作教学。以学生为中心的立体教学模式强调输入与输出相结合,写作过程与结果并重,采用多样化的学习模式,是英语写作教学的有益尝试,必将产生良好的效果。 相似文献
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Jun Suzuki 《Research Policy》2011,40(7):986-1000
There is a considerable volume of prior research on the relationship between innovation and patents. Those research studies reveal that patents contain a great deal of noise, and unless a correction is made in terms of the value of individual patents, a simple count of the number of patents does not constitute a very useful indicator. From research that has been conducted for the purpose of finding such an indicator to show the value of individual patents (that is, research to identify the characteristics of valuable patents), many kinds of value indicators have been proposed. Nevertheless, research hitherto has focused primarily on business or private value derived from the possession of patents, and little attention has been paid to value in terms of technical knowledge or social value. In a survey of inventors conducted by RIETI in 2007, terminology describing broad concepts was used when questioning inventors about the value of individual patents, and this has provided an excellent opportunity to analyze the multiple factors lying behind the value of patents and how they impact one another.The purpose of this research is to use data from the RIETI survey of inventors and structural equation modeling methods to elucidate the relationships between the technological and business value of patents, and the latent factors that influence them. The findings show that a scientific-technological motive for inventors would have a positive effect on both the business and technological value, meanwhile, the monetary or promotion motive would not have any direct effects on the value of a patent. The model also suggests that academic linkage would have a strong positive effect on the technological value but a weak negative effect on the business value. Furthermore, these relationships differ more markedly according to technological field. 相似文献
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随着我国市场经济体制的建立,广义货币供应量(M2)既是监测指标,又是调控指标。从理论和实证分析,M2增长与国内生产总值增长和物价增长密切相关,M2增长对GDP增长具有能动作用。M2增长应控制在一个较高水平的稳定区间,以促使中国经济稳定的增长,缓解当前人民币升值的压力。 相似文献
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周赟 《中国科学院研究生院学报》2007,24(6):725-728
设K为代数闭域k的有限生成扩域.C:f(x)=ayn为K上曲线,其中f是k上至少有3个单零点的多项式且n>3是正整数,n不是域k的特征的倍数,再设a■Kn,那么曲线C不能定义在k上,即曲线C:(x)=ayn不会k(a)同构于一条k上的曲线. 相似文献
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论建立专利预警机制的必要性和预警模型的构建 总被引：2，自引：0，他引：2
戚淳 《科学学与科学技术管理》2008,29(1):16-20
近年来,我国和西方主要国家以技术贸易壁垒形式爆发的贸易摩擦呈上升趋势,其直接和间接损失将对我国经济安全和发展产生长远的作用.通过整合各方面资源,形成一个集专利信息收集、分析、发布和反馈于一体的专利预警体系十分必要.就建立专利预警机制的必要性和其中的困难进行分析,并提出了一个建立预警机制的人工智能模型. 相似文献
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成层地基固结性状中不同定义平均固结度研究分析 总被引：1，自引：0，他引：1
一维地基固结理论从传统的太沙基一维固结理论到成层地基一维固结理论中。由于实际地基大多由性质不一的成状土组成,成层地基固结度计算一直为人们所关注。固结度有按沉降(压缩量)定义的地基总平均固结度和按作用的平均有效应力(或所消散的平均孔压)定义(简称按平均孔压定义)的地基总平均固结度。但对实际工程对它们的大小比较讨论甚少,对实际的选取应用没有理论上的支持。本文从一般均质地基的固结度定义入手,在相对比较理想的土体条件假设下,系统的比较了它们的大小区别,并在单面排水条件下结论Us>Up是一定成立的,并且根据以有的半理论解编制了相应的计算程序来说明本文的正确性。 相似文献
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罗彻斯特是桑菲尔德的男主人,他有文化,但固执傲慢,按他的性格,不可能跟一个没有知识的人进行对话,更不用说心灵的交流了.然而,作为一个既无姿色又无财产的家庭教师,简爱为什么会爱上他而她又被这样一个傲慢的男主人所爱呢?作者将就这一问题与大家探讨。 相似文献
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面向科学复杂性的科学学方法创新 总被引：2，自引：1，他引：1
科学是一个复杂系统,科学复杂性是科学学所面对的一个挑战.为此,需要加强科学学方法的创新.借助复杂网络文献的可视化图谱,在阐述科学复杂性特点的基础上,提出了科学学思维方式的变革,探讨了从微观到宏观的科学计量学方法创新思路,并应用蚁群觅食模型对微观到宏观的知识计量方法进行了简要论述,论证了模型的可行性. 相似文献