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1.
This study examined the effects of caffeine, co-ingested with a high fat meal, on perceptual and metabolic responses during incremental (Experiment 1) and endurance (Experiment 2) exercise performance. Trained participants performed three constant-load cycling tests at approximately 73% of maximal oxygen uptake (VO2max) for 30 min at 20 degrees C (Experiment 1, n = 8) and to the limit of tolerance at 10 degrees C (Experiment 2, n = 10). The 30 min constant-load exercise in Experiment 1 was followed by incremental exercise (15 W . min-1) to fatigue. Four hours before the first test, the participants consumed a 90% carbohydrate meal (control trial); in the remaining two tests, the participants consumed a 90% fat meal with (fat + caffeine trial) and without (fat-only trial) caffeine. Caffeine and placebo were randomly assigned and ingested 1 h before exercise. In both experiments, ratings of perceived leg exertion were significantly lower during the fat + caffeine than fat-only trial (Experiment 1: P < 0.001; Experiment 2: P < 0.01). Ratings of perceived breathlessness were significantly lower in Experiment 1 (P < 0.01) and heart rate higher in Experiment 2 (P < 0.001) on the fat + caffeine than fat-only trial. In the two experiments, oxygen uptake, ventilation, blood [glucose], [lactate] and plasma [glycerol] were significantly higher on the fat + caffeine than fat-only trial. In Experiment 2, plasma [free fatty acids], blood [pyruvate] and the [lactate]:[pyruvate] ratio were significantly higher on the fat + caffeine than fat-only trial. Time to exhaustion during incremental exercise (Experiment 1: control: 4.9, s = 1.8 min; fat-only: 5.0, s = 2.2 min; fat + caffeine: 5.0, s = 2.2 min; P > 0.05) and constant-load exercise (Experiment 2: control: 116 (88 - 145) min; fat-only: 122 (96 - 144) min; fat + caffeine: 127 (107 - 176) min; P > 0.05) was not different between the fat-only and fat + caffeine trials. In conclusion, while a number of metabolic responses were increased during exercise after caffeine ingestion, perception of effort was reduced and this may be attributed to the direct stimulatory effect of caffeine on the central nervous system. However, this caffeine-induced reduction in effort perception did not improve exercise performance.  相似文献   

2.
Abstract

Following fixed-duration exercise of submaximal intensity, caffeine ingestion is associated with an attenuation of the exercise-induced decline in N-formyl-methionyl-phenyl-alanine (f-MLP) stimulated neutrophil oxidative burst. However, the response following high-intensity exhaustive exercise is unknown. Nine endurance-trained male cyclists ingested 6 mg caffeine or placebo per kilogram of body mass 60 min before cycling for 90 min at 70% of maximal oxygen consumption ([Vdot]O2max) and then performing a time-trial requiring an energy expenditure equivalent to 30 min cycling at 70% maximum power output. Time-trial performance was 4% faster in the caffeine than in the placebo trial (P = 0.043). Caffeine was associated with an increased plasma adrenaline concentration after 90 min of exercise (P = 0.046) and immediately after the time-trial (P = 0.02). Caffeine was also associated with an increased serum caffeine concentration (P < 0.01) after 90 min of exercise and immediately after the time-trial, as well as 1 h after the time-trial. However, the f-MLP-stimulated neutrophil oxidative burst response fell after exercise in both trials (P = 0.002). There was no effect of caffeine on circulating leukocyte or neutrophil counts, but the lymphocyte count was significantly lower on caffeine (20%) after the time-trial (P = 0.003). Our results suggest that high-intensity exhaustive exercise negates the attenuation of the exercise-induced decrease in neutrophil oxidative burst responses previously observed when caffeine is ingested before exercise of fixed duration and intensity. This may be associated with the greater increase in adrenaline concentration observed in the present study.  相似文献   

3.
Abstract

It is not known if ergogenic effects of caffeine ingestion in athletic groups occur in the sedentary. To investigate this, we used a counterbalanced, double-blind, crossover design to examine the effects of caffeine ingestion (6 mg · kg?1 body-mass) on exercise performance, substrate utilisation and perceived exertion during 30 minutes of self-paced stationary cycling in sedentary men. Participants performed two trials, one week apart, after ingestion of either caffeine or placebo one hour before exercise. Participants were instructed to cycle as quickly as they could during each trial. External work (J · kg?1) after caffeine ingestion was greater than after placebo (P = 0.001, effect size [ES] = 0.3). Further, heart rate, oxygen uptake and energy expenditure during exercise were greater after caffeine ingestion (P = 0.031, ES = 0.4; P = 0.009, ES = 0.3 and P = 0.018, ES = 0.3; respectively), whereas ratings of perceived exertion and respiratory exchange ratio values did not differ between trials (P = 0.877, ES = 0.1; P = 0.760, ES = 0.1; respectively). The ability to do more exercise after caffeine ingestion, without an accompanying increase in effort sensation, could motivate sedentary men to participate in exercise more often and so reduce adverse effects of inactivity on health.  相似文献   

4.
Abstract

The aim of this study was to assess the effect of caffeine ingestion on 8 km run performance using an ecologically valid test protocol. A randomized double-blind crossover study was conducted involving eight male distance runners. The participants ran an 8 km race 1 h after ingesting a placebo capsule, a caffeine capsule (3 mg · kg?1 body mass) or no supplement. Heart rate was recorded at 5 s intervals throughout the race. Blood lactate concentration and ratings of perceived exertion were recorded after exercise. A repeated-measures analysis of variance (ANOVA) identified a significant treatment effect for 8 km performance time (P < 0.05); caffeine resulted in a mean improvement of 23.8 s (95% confidence interval [CI] = 13.1 to 34.5 s) in 8 km performance time (1.2% improvement, 95% CI = 0.7 to 1.8%). In addition, a two-way (time × condition) repeated-measures ANOVA identified a significantly higher blood lactate concentration 3 min after exercise during the caffeine trial (P < 0.05). We conclude that ingestion of 3 mg · kg?1 body mass of caffeine can improve absolute 8 km run performance in an ecologically valid race setting.  相似文献   

5.
This study examined effects of 4 weeks of caffeine supplementation on endurance performance. Eighteen low-habitual caffeine consumers (<75 mg · day?1) were randomly assigned to ingest caffeine (1.5–3.0 mg · kg?1day?1; titrated) or placebo for 28 days. Groups were matched for age, body mass, V?O2peak and Wmax (P > 0.05). Before supplementation, all participants completed one V?O2peak test, one practice trial and 2 experimental trials (acute 3 mg · kg?1 caffeine [precaf] and placebo [testpla]). During the supplementation period a second V?O2peak test was completed on day 21 before a final, acute 3 mg · kg?1 caffeine trial (postcaf) on day 29. Trials consisted of 60 min cycle exercise at 60% V?O2peak followed by a 30 min performance task. All participants produced more external work during the precaf trial than testpla, with increases in the caffeine (383.3 ± 75 kJ vs. 344.9 ± 80.3 kJ; Cohen’s d effect size [ES] = 0.49; P = 0.001) and placebo (354.5 ± 55.2 kJ vs. 333.1 ± 56.4 kJ; ES = 0.38; P = 0.004) supplementation group, respectively. This performance benefit was no longer apparent after 4 weeks of caffeine supplementation (precaf: 383.3 ± 75.0 kJ vs. postcaf: 358.0 ± 89.8 kJ; ES = 0.31; P = 0.025), but was retained in the placebo group (precaf: 354.5 ± 55.2 kJ vs. postcaf: 351.8 ± 49.4 kJ; ES = 0.05; P > 0.05). Circulating caffeine, hormonal concentrations and substrate oxidation did not differ between groups (all P > 0.05). Chronic ingestion of a low dose of caffeine develops tolerance in low-caffeine consumers. Therefore, individuals with low-habitual intakes should refrain from chronic caffeine supplementation to maximise performance benefits from acute caffeine ingestion.  相似文献   

6.
Abstract

Nine males cycled at 53% (s = 2) of their peak oxygen uptake ([Vdot]O2peak) for 90 min (dry bulb temperature: 25.4°C, s = 0.2; relative humidity: 61%, s = 3). One litre of flavoured water at 10 (cold), 37 (warm) or 50°C (hot) was ingested 30 – 40 min into exercise. Immediately after the 90 min of exercise, participants cycled at 95%[Vdot]O2peak to exhaustion to assess exercise capacity. Rectal and mean skin temperatures and heart rate were recorded. The gradient of rise in rectal temperature was influenced (P < 0.01) by drink temperature. Mean skin temperature was highest in the hot trial (cold trial: 34.2°C, s = 0.5; warm trial: 34.4°C, s = 0.5; hot trial: 34.7°C, s = 0.6; P < 0.01). Significant differences were observed in heart rate (cold trial: 132 beats · min?1, s = 13; warm trial: 134 beats · min?1, s = 12; hot trial: 139 beats · min?1, s = 13; P < 0.05). Exercise capacity was similar between trials (cold trial: 234 s, s = 69; warm trial: 214 s, s = 52; hot trial: 203 s, s = 53; P = 0.562). The heat load and debt induced via drinking resulted in appropriate thermoregulatory reflexes during exercise leading to an observed heat content difference of only 33 kJ instead of the predicted 167 kJ between the cold and hot trials. These results suggest that there may be a role for drink temperature in influencing thermoregulation during exercise.  相似文献   

7.
Abstract

In this study, we examined thermoregulatory responses to ingestion of separate aliquots of drinks at different temperatures during low-intensity exercise in conditions of moderate heat stress. Eight men cycled at 50% (s = 3) of their peak oxygen uptake ([Vdot]O2peak) for 90 min (dry bulb temperature: 25.3°C, s = 0.5; relative humidity: 60%, s = 5). Four 400-ml aliquots of flavoured water at 10°C (cold), 37°C (warm) or 50°C (hot) were ingested after 30, 45, 60, and 75 min of exercise. Immediately after the 90 min of exercise, participants cycled at 95%[Vdot]O2peak to exhaustion to assess exercise capacity. There were no differences between trials in rectal temperature at the end of the 90 min of exercise (cold: 38.11°C, s = 0.30; warm: 38.10°C, s = 0.33; hot: 38.21°C, s = 0.30; P = 0.765). Mean skin temperature between 30 and 90 min tended to be influenced by drink temperature (cold: 34.49°C, s = 0.64; warm: 34.53°C, s = 0.69; hot: 34.71°C, s = 0.48; P = 0.091). Mean heart rate from 30 to 90 min was higher in the hot trial (129 beats · min?1, s = 7; P < 0.05) than on the cold (124 beats · min?1, s = 9) and warm trials (126 beats · min?1, s = 8). Ratings of thermal sensation were higher on the hot trial than on the cold trial at 35 and 50 min (P < 0.05). Exercise capacity was similar between trials (P = 0.963). The heat load and debt induced by periodic drinking resulted in similar body temperatures during low-intensity exercise in conditions of moderate heat stress due to appropriate thermoregulatory reflexes.  相似文献   

8.
Abstract

In this study, we examined the effects of different work:rest durations during 20 min intermittent treadmill running and subsequent performance. Nine males (mean age 25.8 years, s = 6.8; body mass 73.9 kg, s = 8.8; stature 1.75 m, s = 0.05; [Vdot]O2max 55.5 ml · kg?1 · min?1, s = 5.8) undertook repeated sprints at 120% of the speed at which [Vdot]O2max was attained interspersed with passive recovery. The work:rest ratio was constant (1:1.5) with trials involving either short (6:9 s) or long (24:36 s) work:rest exercise protocols (total exercise time 8 min). Each trial was followed by a performance run to volitional exhaustion at the same running speed. Testing order was randomized and counterbalanced. Heart rate, oxygen consumption, respiratory exchange ratio, and blood glucose were similar between trials (P > 0.05). Blood lactate concentration was greater during the long than the short exercise protocol (P < 0.05), whereas blood pH was lower during the long than the short exercise protocol (7.28, s = 0.11 and 7.30, s = 0.03 at 20 min, respectively; P < 0.05). Perceptions of effort were greater throughout exercise for the long than the short exercise protocol (16.6, s = 1.4 and 15.1, s = 1.6 at 20 min, respectively; P < 0.05) and correlated with blood lactate (r = 0.43) and bicarbonate concentrations (r = ?0.59; P < 0.05). Although blood lactate concentration at 20 min was related to performance time (r = ?0.56; P < 0.05), no differences were observed between trials for time to exhaustion (short exercise protocol: 95.8 s, s = 30.0; long exercise protocol: 92.0 s, s = 37.1) or physiological responses at exhaustion (P > 0.05). Our results demonstrate that 20 min of intermittent exercise involving a long work:rest duration elicits greater metabolic and perceptual strain than intermittent exercise undertaken with a short work:rest duration but does not affect subsequent run time to exhaustion.  相似文献   

9.
Skin and core tissue cooling modulates skeletal muscle oxygenation at rest. Whether tissue cooling also influences the skeletal muscle deoxygenation response during exercise is unclear. We evaluated the effects of skin and core tissue cooling on skeletal muscle blood volume and deoxygenation during sustained walking and running. Eleven male participants walked or ran six times on a treadmill for 60 min in ambient temperatures of 22°C (Neutral), 0°C for skin cooling (Cold 1), and at 0°C following a core and skin cooling protocol (Cold 2). Difference between oxy/deoxygenated haemoglobin ([diffHb]: deoxygenation index) and total haemoglobin content ([tHb]: total blood volume) in the vastus lateralis (VL) muscle was measured continuously. During walking, lower [tHb] was observed at 1 min in Cold 1 and Cold 2 vs. Neutral (P?0.05). Lower [diffHb] was seen at 1 and 10 min in Cold 2 vs. Neutral by 13.5 ± 1.2 µM and 15.3 ± 1.4 µM and Cold 1 by 10.4 ± 3.1 µM and 11.1 ± 4.1 µM, respectively (P?0.05). During running, [tHb] was lower in Cold 2 vs. Neutral at 10 min only (P = 0.004). [diffHb] was lower at 1 min in Cold 2 by 11.3 ± 3.1 µM compared to Neutral and by 13.5 ± 2.8 µM compared to Cold 1 (P?0.001). Core tissue cooling, prior to exercise, induced greater deoxygenation of the VL muscle during the early stages of exercise, irrespective of changes in blood volume. Skin cooling alone, however, did not influence deoxygenation of the VL during exercise.  相似文献   

10.
The effects of sodium phosphate and caffeine supplementation were assessed on repeated-sprint ability. Using a randomised, double-blind, Latin-square design, 12 female, team-sport players participated in four trials: (1) sodium phosphate and caffeine, (2) sodium phosphate and placebo (for caffeine), (3) caffeine and placebo (for sodium phosphate) and (4) placebo (for sodium phosphate and caffeine), with ~21 days separating each trial. After each trial, participants performed a simulated team-game circuit (4 × 15 min quarters) with 6 × 20-m repeated-sprints performed once before (Set 1), at half-time (Set 2), and after end (Set 3). Total sprint times were faster after sodium phosphate and caffeine supplementation compared with placebo (Set 1: P = 0.003; Set 2: d = ?0.51; Set 3: P < 0.001; overall: P = 0.020), caffeine (Set 3: P = 0.004; overall: P = 0.033) and sodium phosphate (Set 3: d = ?0.67). Furthermore, total sprint times were faster after sodium phosphate supplementation compared with placebo (Set 1: d = ?0.52; Set 3: d = ?0.58). Best sprint results were faster after sodium phosphate and caffeine supplementation compared with placebo (Set 3: P = 0.007, d = ?0.90) and caffeine (Set 3: P = 0.024, d = ?0.73). Best sprint times were also faster after sodium phosphate supplementation compared with placebo (d = ?0.54 to ?0.61 for all sets). Sodium phosphate and combined sodium phosphate and caffeine loading improved repeated-sprint ability.  相似文献   

11.
Abstract

Glutamine enhances the exercise-induced expansion of the tricarboxylic acid intermediate pool. The aim of the present study was to determine whether oral glutamine, alone or in combination with hyperoxia, influenced oxidative metabolism and cycle time-trial performance. Eight participants consumed either placebo or 0.125 g · kg body mass?1 of glutamine in 5 ml · kg body mass?1 placebo 1 h before exercise in normoxic (control and glutamine respectively) or hyperoxic (FiO2 = 50%; hyperoxia and hyperoxia + glutamine respectively) conditions. Participants then cycled for 6 min at 70% maximal oxygen uptake ([Vdot]O2max) immediately before completing a brief high-intensity time-trial (～4 min) during which a pre-determined volume of work was completed as fast as possible. The increment in pulmonary oxygen uptake during the performance test (Δ[Vdot]O2max, P = 0.02) and exercise performance (control: 243 s, s x  = 7; glutamine: 242 s, s x  = 3; hyperoxia: 231 s, s x  = 3; hyperoxia + glutamine: 228 s, s x  = 5; P < 0.01) were significantly improved in hyperoxic conditions. There was some evidence that glutamine ingestion increased Δ[Vdot]O2max in normoxia, but not hyperoxia (interaction drink/FiO2, P = 0.04), but there was no main effect or impact on performance. Overall, the data show no effect of glutamine ingestion either alone or in combination with hyperoxia, and thus no limiting effect of the tricarboxylic acid intermediate pool size, on oxidative metabolism and performance during maximal exercise.  相似文献   

12.
Abstract

The aim of this study was to investigate the effect of ingesting a carbohydrate-electrolyte solution, during the 90-min Loughborough Intermittent Shuttle Test, on soccer skill performance. Seventeen male soccer players ingested either a 6.4% carbohydrate-electrolyte solution or placebo solution equivalent to 8 ml · kg?1 body mass before exercise and 3 ml · kg?1 body mass after every 15 min of exercise, in a double-blind randomized cross-over design, with the trials separated by 7 days. The evening before the main trial, the participants performed glycogen-reducing exercise on a cycle ergometer (80 min at 70%[Vdot]O2max) and were then fed a low-carbohydrate meal. After a 12-h overnight fast, they performed The Loughborough Soccer Passing Test before and after every 15 min of exercise. Analysis of the combined skill test data showed a significant time effect (P = 0.001) with differences between 0–45 and 75–90 min (P < 0.05). There was a 3% reduction in skill performance from before to after exercise in the carbohydrate-electrolyte trial, whereas in the placebo trial the decrease was 14% (P = 0.07). In conclusion, skill performance during the simulated soccer activity appeared to deteriorate in the last 15–30 min of exercise. However, providing 52 g · h?1 carbohydrate during exercise showed a tendency to better maintain soccer skill performance than a taste-matched placebo.  相似文献   

13.
Two experiments (n = 10) were conducted to determine the effects of roller massager (RM) on ankle plantar flexor muscle recovery after exercise-induced muscle damage (EIMD). Experiment 1 examined both functional [i.e., ankle plantar flexion maximal isometric contraction and submaximal (30%) sustained force; ankle dorsiflexion maximal range of motion and resistance to stretch; and medial gastrocnemius pain pressure threshold] and morphological [cross-sectional area, thickness, fascicle length, and fascicle angle] variables, before and immediately, 1, 24, 48, and 72 h after an EIMD stimulus. Experiment 2 examined medial gastrocnemius deoxyhaemoglobin concentration kinetics before and 48 h after EIMD. Participants performed both experiments twice: with (RM) and without (no-roller massager; NRM) the application of a RM (6 × 45 s; 20-s rest between sets). RM intervention did not alter the functional impairment after EIMD, as well as the medial gastrocnemius morphology and oxygenation kinetics (P > 0.05). Although, an acute increase of ipsilateral (RM = + 19%, NRM = ?5%, P = 0.032) and a strong tendency for contralateral (P = 0.095) medial gastrocnemius pain pressure threshold were observed. The present results suggest that a RM has no effect on plantar flexors performance, morphology, and oxygenation recovery after EIMD, except for muscle pain pressure threshold (i.e., a soreness).  相似文献   

14.
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15.
Abstract

The purpose of this study was to compare the effects of two practical precooling techniques (skin cooling vs. skin + core cooling) on cycling time trial performance in warm conditions. Six trained cyclists completed one maximal graded exercise test ([Vdot]O2peak 71.4 ± 3.2 ml · kg?1 · min?1) and four ～40 min laboratory cycling time trials in a heat chamber (34.3°C ± 1.1°C; 41.2% ± 3.0% rh) using a fixed-power/variable-power format. Cyclists prepared for the time trial using three techniques administered in a randomised order prior to the warm-up: (1) no cooling (control), (2) cooling jacket for 40 min (jacket) or (3) 30-min water immersion followed by a cooling jacket application for 40 min (combined). Rectal temperature prior to the time trial was 37.8°C ± 0.1°C in control, similar in jacket (37.8°C ± 0.3°C) and lower in combined (37.1°C ± 0.2°C, P < 0.01). Compared with the control trial, time trial performance was not different for jacket precooling (?16 ± 36 s, ?0.7%; P = 0.35) but was faster for combined precooling (?42 ± 25 s, ?1.8%; P = 0.009). In conclusion, a practical combined precooling strategy that involves immersion in cool water followed by the use of a cooling jacket can produce decrease in rectal temperature that persist throughout a warm-up and improve laboratory cycling time trial performance in warm conditions.  相似文献   

16.
Abstract

Ghrelin is a hormone that stimulates hunger. Intense exercise has been shown to temporarily suppress hunger after exercise. In the present study, we investigated whether post-exercise hunger suppression is mediated by reduced plasma total ghrelin concentrations. Nine men and nine women participated in the study. Their mean physical characteristics were as follows: age 24.8 (s x  = 0.9) years, body mass index 22.9 (s x  = 0.6) kg · m?2, maximal oxygen uptake ([Vdot]O2max) 57.7 (s x  = 2.2) ml · kg?1 · min?1. The participants completed two 3-h trials (exercise and control) on separate days in a randomized balanced design after overnight fasts. The exercise trial involved a 1-h treadmill run at 73.5% of [Vdot]O2max followed by 2 h of rest. The control trial consisted of 3 h of rest. Blood samples were collected at 0, 0.5, 1, 1.5, 2, and 3 h. Total ghrelin concentrations were determined from plasma. Hunger was assessed following blood sampling using a 15-point scale. The data were analysed using repeated-measures analysis of variance. Hunger scores were lower in the exercise trial than in the control trial (trial, P = 0.009; time, P < 0.001; trial × time, P < 0.001). Plasma total ghrelin concentrations did not differ between trials. These findings indicate that treadmill running suppresses hunger but this effect is not mediated by changes in plasma total ghrelin concentration.  相似文献   

17.
The aim of this study was to compare the effect of low-load resistance exercise (LLRE) with continuous and intermittent blood flow restriction (BFR) on the creatine kinase (CK), lactate dehydrogenase (LDH), protein carbonyl (PC), thiobarbituric acid-reactive substance (TBARS) and uric acid (UA) levels in military men. The study included 10 recreationally trained men aged 19 ± 0.82 years who underwent the following experimental protocols in random order on separate days (72–96 h): 4 LLRE sessions at a 20% 1RM (one-repetition maximum [1RM]) with continuous BFR (LLRE + CBFR); 4 LLRE sessions at 20% 1RM with intermittent BFR (LLRE + IBFR) and 4 high-intensity resistance exercise (HIRE) sessions at 80% 1RM. The CK and LDH (markers of muscle damage) levels were measured before exercise (BE), 24 h post-exercise and 48 h post-exercise, and the PC, TBARS and UA (markers of oxidative stress) levels were measured BE and immediately after each exercise session. There was a significant increase in CK in the HIRE 24 post-exercise samples compared with the LLRE + CBFR and LLRE + IBFR (P = 0.035, P = 0.036, respectively), as well as between HIRE 48 post-exercise and LLRE + CBFR (P = 0.049). Additionally, there was a significant increase in CK in the LLRE + CBFR samples BE and immediately after each exercise (Δ = 21.9%) and in the HIRE samples BE and immediately after each exercise, BE and 24 post-exercise, and BE and 48 post-exercise (Δ values of 35%, 177.6%, and 177.6%, respectively). However, there were no significant changes in LDH, PC, TBARS, and UA between the protocols (P > 0.05). Therefore, a physical exercise session with continuous or intermittent BFR did not promote muscle damage; moreover, neither protocol seemed to affect the oxidative stress markers.  相似文献   

18.
An increase in salivary leukocytes may contribute to the exercise-induced increase in salivary antimicrobial proteins (AMPs). However, exercise-induced changes in salivary leukocytes have not been studied. The purpose of the study was to describe salivary leukocyte changes with exercise. Participants (n = 11, 20.3 ± 0.8 years, 57.2 ± 7.6 ml kg?1 min?1 peak oxygen uptake ((VO) ?2peak), 11.1 ± 3.9% body fat) ran for 45 min at 75% of VO2peak. Stimulated saliva (12 mL) was collected pre- and immediately post exercise. Saliva was filtered through a 30 µm filter before analysis of leukocytes (CD45+), granulocytes (CD45+CD15+), monocytes (CD45+CD14+), T-cells (CD45+CD3+), and B-cells (CD45+CD20+) using flow cytometry. Saliva was analysed for Lysozyme (Lys) using ELISA. Exercise did not alter any leukocyte subset. The major constituent of leukocytes pre-exercise were granulocytes (57.9 ± 30.3% compared with monocytes: 5.1 ± 2.7%, T-cells: 17.1 ± 8.9%, B-cells: 12.1 ± 10.2%) (P < 0.05). In a subset of n = 6, Lys secretion rate increased after exercise (pre: 5,170 ± 5,215 ng/min; post: 7,639 ± 4,140 ng/min) (P < 0.05). Exercise does not result in increased granulocytes, but does increase Lys. Further, these data suggest that an increase in salivary leukocytes is not needed to increase Lys.  相似文献   

19.
We examined the influence of caffeine supplementation on cognitive performance and perceptual responses in female team-game players taking low-dose monophasic oral contraceptives of the same hormonal composition. Ten females (24 ± 4 years; 59.7 ± 3.5 kg body mass; 2–6 training sessions per week) took part in a randomised, double-blind, placebo-controlled crossover-design trial. A 90-min intermittent treadmill-running protocol was completed 60 min following ingestion of a capsule containing either 6 mg ? kg?1 anhydrous caffeine or artificial sweetener (placebo). Perceptual responses (ratings of perceived exertion (RPE), feeling scale (FS), felt arousal scale (FAS)), mood (profile of mood states (POMS)) and cognitive performance (Stroop test, choice reaction time (CRT)) were completed before, during and after the exercise protocol, as well as after ~12 h post exercise. Caffeine ingestion significantly enhanced the ratings of pleasure (P = 0.008) and arousal (P = 0.002) during the exercise protocol, as well as increased vigour (POMS; P = 0.007), while there was a tendency for reduced fatigue (POMS; P = 0.068). Caffeine ingestion showed a tendency to decrease RPE (P = 0.068) and improve reaction times in the Stroop (P = 0.072) and CRT (P = 0.087) tests. Caffeine supplementation showed a positive effect on perceptual parameters by increasing vigour and a tendency to decrease fatigue during intermittent running activity in female games players taking low-dose monophasic oral contraceptive steroids (OCS).  相似文献   

20.
Abstract

The badminton serve requires great skill and may be affected by fatigue. The aim of the present study was to determine whether carbohydrate ingestion affects badminton performance. Nine male badminton players (age 25 ± 7 years, mass 80.6 ± 8.0 kg) attended the laboratory on three occasions. The first visit involved an incremental exercise test to exhaustion to determine peak heart rate. Participants were given 1 L of a carbohydrate-electrolyte drink or a matched placebo during the experimental trials. The accuracy of 10 long and 10 short serves was determined before and after exercise. The fatiguing exercise was 33 min in duration (83 ± 10% and 84 ± 8% peak heart rate for the placebo and carbohydrate trial respectively). Capillary blood samples (20 μL) were taken before and after exercise for determination of blood glucose and lactate. There was deterioration in long serve accuracy with fatigue (P = 0.002), which carbohydrate ingestion had a tendency to prevent (P = 0.077). There was no effect of fatigue (P = 0.402) or carbohydrate ingestion (P = 0.109) on short serve accuracy. There was no difference in blood glucose concentration between trials (P = 0.851). Blood lactate concentration was higher during the placebo trial (P = 0.016). These results suggest that only the long serve is influenced by fatigue and carbohydrate had a tendency to prevent the deterioration in performance.  相似文献   

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