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1.
Beer fermentation is a dynamic process that must be guided along a temperature profile to obtain the desired results. Ant colony system algorithm was applied to optimize the kinetic model of this process. During a fixed period of fermentation time, a series of different temperature profiles of the mixture were constructed. An optimal one was chosen at last. Optimal temperature profile maximized the final ethanol production and minimized the byproducts concentration and spoilage risk. The satisfactory results obtained did not require much computation effort.  相似文献   

2.
目的:研究酶法降解玉米芯的最佳工艺和酵母菌发酵的最佳工艺.方法:采用纤维素酶对玉米芯进行酶解,采用酵母菌对酶解液进行发酵.结果:酶解条件:纤维素酶0.6mL,pH值为5~6,温度40℃,Tween-80添加量为1%,微量元素的添加量为0.5%,酶解48h后还原糖浓度达到最大值;发酵条件:活化后酵母菌的接种量40mL,发酵温度30℃,发酵36h后,乙醇产量的最高.结论:在较佳的酶解和较佳的发酵条件下,可显著提高酶解率和发酵率.  相似文献   

3.
论述了营养基质、温度、PH值、溶解氧、接种量等因子对虫草菌发酵生产的影响 ,并对因子的控制进行了分析  相似文献   

4.
为了探索具有食品安全性的亚硝酸盐去除方法,研究了深红酵母降解亚硝酸盐的工艺条件。通过摇瓶培养24h的实验,确定了降解亚硝酸盐的最佳条件为:温度30℃,pH4.0,转速200r/min,接种量10%。在最佳条件下,亚硝酸盐降解率可达98.51%。  相似文献   

5.
以火棘为原料,对采用固定化醋酸菌酿造火棘果醋的醋酸发酵工艺进行了研究。对发酵温度、接种量、酒精度三个影响因素进行正交试验,结果表明,固定化醋酸菌酿造火棘果醋的最佳工艺为:发酵温度34℃,接种量10%,酒精度7%。所得火棘果醋醋香浓郁,酸甜适度,香气自然、协调,是一种值得开发的保健果醋。  相似文献   

6.
为探讨农用植物酵素堆肥(简称酵素堆肥)腐熟度进程,指导农民自制安全、稳定、无害化堆肥,设置添加土著酵素腐熟菌剂和无添加两组、采用高温好氧发酵堆肥工艺进行堆肥实验,对堆体温度、pH、电导率、E4/E6、发芽率、物理感官等腐熟度指标进行研究。结果表明:酵素堆肥与清水堆肥发酵总体趋势基本一致,温度先升高后降低,pH、电导率(EC)、E4/E6、种子发芽率(SG)均随堆肥进行逐渐上升;添加菌剂有利于提高并保持堆温,加快并提高种子发芽率,促进堆肥矿化和腐殖化进程,对pH、EC、E4/E6等腐熟度指标影响不大;主发酵结束时物料布满菌丝,但种子发芽率和E4/E6未达到腐熟度标准。  相似文献   

7.
葡萄酒发酵过程放热量大,会使发酵介质的温度升高而影响酒的质量,因而控温发酵是酿造高品质葡萄酒的一项重要技术措施。为此,对红葡萄酒发酵过程的发热特性进行了实验研究。首先在无温控状况下进行自然发酵,研究发热量的变化关系,确定发酵过程中变化的内热源项。随后进行控温发酵实验,得到控温条件下的发酵介质温度变化曲线,并分析了控温范围等因素对发酵过程传热的影响。  相似文献   

8.
目的:初步摸索出一条适合工业化开发、高效的、可溶表达结核分枝杆菌MTB8.1蛋白的发酵工艺。方法:利用菌株pUC18/MTB8.1/DH10B为发酵株,通过对培养基组成和发酵参数进行系统研究,从而获得一全新的发酵工艺。结果:重组结核分枝杆菌MTB8.1蛋白通过本发酵工艺的表达,单位体积发酵液可溶性蛋白的产量大幅提高,每升发酵液纯化得到可溶性蛋白约30 mg左右。结论:初步摸索出一条较好的MTB8.1在大肠埃希菌系统中的可溶性表达的途径,为其工业化生产奠定了理论基础。  相似文献   

9.
采用自行设计的发酵装置,通过温度对挥发性脂肪酸的影响,从而进行了消化温度对柠檬果渣厌氧消化过程中产气量的影响研究.实验表明,在中温(35℃)和高温(55℃)两种消化条件下及pH接近中性时,柠檬果渣消化反应器分别于第7天和第5天开始进入产甲烷阶段,最高产气量分别为44mi和46mi,高温比中温的产气量提高了4.5%.由此可见,高温消化比中温消化缩短了消化时间,提高了产气量,高温消化是提高柠檬果渣厌氧消化产气量的有效方法.  相似文献   

10.
以桑葚为主要原料,以酿酒活性干酵母为桑葚酒发酵菌种,通过正交试验的研究,可以确定桑葚酒发酵的最佳工艺参数为:总糖含量22%、发酵温度28℃、接种量0.1%、SO2添加量40ppm。影响桑葚酒发酵因素的主次顺序为总糖含量(A)〉发酵温度(B)〉接种量(C)〉SO2添加量(D),桑葚酒具有多种生物活性成分,是一种值得开发的果酒。  相似文献   

11.
Study of the effect of dissolved oxygen and shear stress on rifamycin B fermentation with A. mediterranei XC 9-25 showed that rifamycin B fermentation with Amycolatoposis mediterranei XC 9-25 needs high dissolved oxygen and is not very sensitive to shearing stress. The scale-up of rifamycin B fermentation with A mediterranei XC 9-25 from a shaking flask to a 15 L fermentor was realized by controlling the dissolved oxygen to above 25% of saturation in the fermentation process, and the potency of rifamycin B fermentation in the 15 L fermentor reached 10 g/L after 6-day batch fermentation. By continuously feeding glucose and ammonia in the fermentation process, the potency of rifamycin B fermentaion in the 15 L fermentor reached 18.67 g/L, which was 86.65% higher than that of batch fermentation. Based on the scale-up principle of constantly aerated agitation power per unit volume, the scale-up of rifamycin B fed-batch fermentation with continuous feed from a 15 L fermentor to a 7 m3 fermentor and further to  相似文献   

12.
Study of the effect of dissolved oxygen and shear stress on rifamycin B fermentation with A. mediterranei XC 9-25 showed that rifamycin B fermentation with Amycolatoposis mediterranei XC 9-25 needs high dissolved oxygen and is not very sensitive to shearing stress. The scale-up of rifamycin B fermentation withA. mediterranei XC 9-25 from a shaking flask to a 15 L fermentor was realized by controlling the dissolved oxygen to above 25% of saturation in the fermentation process, and the potency of rifamycin B fermentation in the 15 L fermentor reached 10 g/L after 6-day batch fermentation.By continuously feeding glucose and ammonia in the fermentation process, the potency of rifamycin B fermentaion in the 15 L fermentor reached 18.67 g/L, which was 86.65% higher than that of batch fermentation. Based on the scale-up principle of constantly aerated agitation power per unit volume, the scale-up ofrifamycin B fed-batch fermentation with continuous feed from a 15 L fermentor to a 7 m3 fermentor and further to a 60 m3 fermentor was realized successfully. The potency of rifamycin B fermentation in the 7 m3 fermentor and in the 60 m3 fermentor reached 17.25 g/L and 19.11 g/L, respectively.  相似文献   

13.
通过实验研究了草莓酸奶的生产工艺条件,并确定了最佳配比及最佳工艺流程。结果表明:以保加利亚杆菌和嗜热链球菌(1∶1)混合菌为发酵菌种,白砂糖添加量为8%,草莓果浆添加量6%,接种量3.5%,发酵时间4 h,发酵温度42℃,产品性能最佳。  相似文献   

14.
在啤酒发酵过程中,利用计算机控制管理系统,通过分段处理的方案,来完成对温度和压力的控制,实现关键技术。该系统结构简单、成本低,扩展性、移植性强,具有推广实用价值。  相似文献   

15.
Study of the effect of dissolved oxygen and shear stress on rifamycin B fermentation with A. mediterranei XC 9–25 showed that rifamycin B fermentation with Amycolatoposis mediterranei XC 9–25 needs high dissolved oxygen and is not very sensitive to shearing stress. The scale-up of rifamycin B fermentation with A. mediterranei XC 9–25 from a shaking flask to a 15 L fermentor was realized by controlling the dissolved oxygen to above 25% of saturation in the fermentation process, and the potency of rifamycin B fermentation in the 15 L fermentor reached 10 g/L after 6-day batch fermentation. By continuously feeding glucose and ammonia in the fermentation process, the potency of rifamycin B fermentation in the 15 L fermentor reached 18.67 g/L, which was 86.65% higher than that of batch fermentation. Based on the scale-up principle of constantly aerated agitation power per unit volume, the scale-up of rifamycin B fed-batch fermentation with continuous feed from a 15 L fermentor to a 7 m3 fermentor and further to a 60 m3 fermentor was realized successfully. The potency of rifamycin B fermentation in the 7 m3 fermentor and in the 60 m3 fermentor reached 17.25 g/L and 19.11 g/L, respectively.  相似文献   

16.
研究甘蔗汁风味醋摇瓶发酵条件,为甘蔗汁风味醋的工业化生产提供依据和参考。以甘蔗汁为原料,通过研究了初始酒精度、初始pH、发酵温度、接种量和转速对醋酸发酵的影响,确定最佳的醋酸摇瓶发酵条件为:初始酒精度6.5%,初始pH5.0,发酵温度30℃,接种量10%,发酵0~72 h的转速200 r/min,发酵72 h以后的转速150 r/min。在此条件下,发酵转化率和产醋速率分别达到97.9%和0.467 g/(L.h)。  相似文献   

17.
基于ANSYS软件,对塑料异型材在定型模内的温度场进行数值模拟.通过分析型材冷却过程中的热传递方式,对计算模型、初始条件和边界条件进行了更符合生产实际的假设或赋值,并根据模拟结果进行冷却水道的布置.  相似文献   

18.
As a kind of aerobic bacteria, Saccharopolyspora spinosa exhibits a high demand for oxygen. In the fermentation process, the methods of increasing ventilation and improving agitation speed are usually adopted to achieve higher values of dissolved oxygen. These methods decrease the efficiency of spinosad biosynthesis.In this study, an improved reactor was designed to solve these problems. The exhaust gas reflux device, impellers,and baffles were improved. Furthermore, we established the kinetic models for the cell growth, substrate consumption and spinosad generation in batch fermentation process. The simulation results were in good agreement with the experimental data. Spinosad production reached 583.86 mg/L after employing the suitable feeding strategy by fed-batch fermentation in the improved reactor, whereas it was only 157.01 mg/L before optimization. The method described can provide insight to strengthen spinosad production and can be extended to the culturing process of filamentous aerobic bacteria.  相似文献   

19.
The production of elastase by Bacillus sp. EL31410 at various temperatures was investigated. In order to study the effect of temperature on elastase fermentation, different cultivation temperatures, ranging from 39 °C to 28 °C, were evaluated in shake flask. The result indicated that 37 °C was best for cell growth at earlier stage; while maximum elastase activity was obtained when the cells were cultivated at 30 °C. This result was verified by batch fermentation in 5-L bioreactor under 37 °C and 30 °C temperature, respectively. The specific cell growth rate at 37 °C was higher than that at 30 °C during earlier stage of cultivation. The maximum value [5.5 U/(h·g DCW)] of elastase formation rate occurred at 24 h at 30 °C compared to 4.6 U/(h·g DCW) at 30 h at 37 °C. Based on these results, two-stage temperature shift strategy and oscillatory temperature cultivation mode were evaluated in the next study. When compared to single temperature of 37 °C or 30 °C, both two-stage temperature shift strategy and oscillatory temperature strategy improved biomass but did not yield the same result as expected for elastase production. The maximum biomass (both 8.6 g/L) was achieved at 30 h at 37 °C, but at 42 h using two-stage temperature cultivation strategy. The highest elastase production (652 U/ml) was observed at 30 °C in batch process. It was concluded that cultivation at constant temperature of 30 °C was appropriate for elastase production by Bacillus sp. EL31410. Project (No. 20276064) supported by the National Natural Science Foundation of China  相似文献   

20.
针对微生物发酵补料生产中自动化程度低和补料量精度差的问题,提出一种基于AGA-BP的微生物发酵过程智能补料控制策略。基于微生物发酵过程的工艺特性,利用BP神经网络建立发酵过程的动态数学模型,并引入自适应遗传算法AGA对BP神经网络动态补料控制模型的权值进行优化。将建立的AGA-BP智能补料控制模型嵌入到ARM11控制器中,计算出当前最佳补料量,并通过无线传输模块对补料执行模块进行远程控制。实验结果表明,菌体浓度在AGA-BP补料策略控制下,相比人工补料提高了8.62%,相比未优化的BP神经网络提高了4.55%。因此,基于AGA-BP的补料策略具有更高的补料控制精度,有助于生物发酵实现自动化生产,提高发酵效率,降低生产成本。  相似文献   

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