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《黑龙江科技信息》2017,(4)
研究观察苯对小鼠骨髓嗜多染红细胞微核的影响,对苯的遗传毒性进行评价。将小鼠随机分为阴性对照组、阳性对照组及低浓度、中浓度、高浓度、3个剂量共5组,每组10只,雌雄各半,采用静式吸入染毒法,3个剂量组每天染毒1次。连续30d。2个对照组放置在另一个染毒柜中,除不染毒外其余处理均同染毒组。阳性对照组小鼠于处死前1d腹腔注射环磷酰胺(60mg/kg/B·W)1次。30d后处死小鼠,采用常规微核实验方法,检测小鼠骨髓嗜多染红细胞微核。以微核率为指标,进行方差分析,判断苯对小鼠骨髓嗜多染红细胞微核的影响。实验组组的微核率要高于阴性和阳性对照组,高剂量组的微核率最高!实验中的苯试剂可促进小鼠骨髓嗜多染红细胞产生微核,具有遗传毒性,且高浓度的苯毒性最强。 相似文献
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采用水培试验的方法,研究不同浓度铜（0、20、40、60、80和100mg/L）胁迫条件下小叶黄杨的生长和生理特征。结果表明,随铜浓度的增加,小叶黄杨的生物量（鲜重）逐渐下降;蛋白质含量呈下降趋势;叶绿素含量先上升后下降;丙二醛的含量逐渐增加;超氧化物歧化酶和过氧化物酶的活性均呈先增加后下降的趋势。 相似文献
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目的 对刺薏复方进行抗辐射功效实验研究,验证其在抗辐射方面具有辅助保护作用.方法 各实验项下的小鼠均给药后第14天以Co60Y射线对小鼠一次性全身照射,分别进行外周血白细胞(W B C)计数,骨髓有核细胞计数,骨髓微核细胞计数,超氧化物歧化酶(SOD)活性测定,血清溶血素水平测定及胸腺、脾脏指数测定等实验.结果 刺薏复方具有促进WBC的恢复,增加股骨骨髓有核细胞数,减少股骨骨髓细胞微核数,提高血中SOD活性,升高血清溶血素水平及胸腺、脾脏指数等作用.结论 刺薏复方具有抗辐射辅助保护作用,且抗辐射作用与给药剂量存在量效正向关系. 相似文献
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四氧嘧啶致小鼠、大鼠糖尿病模型研究 总被引：1，自引：0，他引：1
目的:研究四氧嘧啶尾静脉注射小鼠和大鼠诱导糖尿病模型,观察不同剂量四氧嘧啶诱导糖尿病模型的稳定性.方法1分别对小鼠尾静脉注射四氧嘧啶60、70、80mg/kg和对大鼠尾静脉注射四氧嘧啶30、40、50mg/kg,测定不同时点小鼠和大鼠的血糖值及体重.结果60、70和80mg/kg四氧嘧啶剂量均可导致小鼠糖尿病模型,但70和80mg/kg剂量组小鼠死亡率较大,而60mg/kg组小鼠糖尿病模型稳定;30mg/kg四氧嘧啶剂量不能造成大鼠糖尿病模型,40和50mg/kg四氧嘧啶剂量均可导致大鼠糖尿病模型,但四氧嘧啶50mg/kg剂量组大鼠死亡率较大,只有40mg/kg组糖尿病大鼠模型稳定.结论:造成小鼠和大鼠糖尿病模型最佳剂量分别为60mg/kg和40mg/kg. 相似文献
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纳米硒对肉鸡肌肉品质的影响 总被引：4，自引：0，他引：4
本实验以亚硒酸钠为对照,研究了纳米硒对肉鸡肌肉品质的影响。岭南黄公母混合雏780羽按饲养试验要求分为13组,每组4个重复,每个重复15羽。将纳米硒和亚硒酸钠2种硒源分别以0.1、0.2、0.3、0.4、0.5、1.0 mg/kg 6个硒水平添加到基础日粮中,基础日粮作对照。结果显示:(1)纳米硒添加浓度在0.20-1.0 mg/kg胸肌滴水损失始终保持在低峰平台。硒源添加浓度在0.40-1.0 mg/kg硒时,纳米硒组胸肌滴水损失显著低于亚硒酸钠组(P<0.05)。(2)硒源添加浓度在0.10-0.40 mg/kg时,两种硒源对胸肌GSH-Px活性的影响无显著差异(P<0.05);在0.50和1.0 mg/kg硒添加水平上,纳米硒组GSH-Px 活性显著高于亚硒酸钠组(P<0.05)。硒源添加浓度在0.40-1.0 mg/kg硒时,纳米硒组T-AOC显著高于亚硒酸钠组(P<0.05),MDA含量显著低于亚硒酸钠组(P<0.05)。(3)不加硒的空白组胸肌硒含量显著低于加硒组。硒源添加浓度在0.10-0.30 mg/kg时,两种硒源对胸肌硒含量的影响无显著差异;在0.40- 1.0 mg/kg硒添加水平上,纳米硒组胸肌硒含量显著高于亚硒酸钠组。上述结果提示,纳米硒的Wein berg剂量-效应的最适剂量范围要宽于亚硒酸钠,高剂量添加时比亚硒酸钠更能提高肌肉组织抗氧化能力,降低肉的滴水损失;肌肉硒沉积率优于亚硒酸钠。 相似文献
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目的:通过复制D-半乳糖所致的衰老小鼠模型,探讨广西产五指毛桃不同提取物的抗衰老作用。方法:将70只实验小鼠随机分为7组:正常组,模型组,维生素E(Vit E)组(100mg/g),五指毛桃水提物高(20g/kg)、低(10g/kg)剂量组,五指毛桃醇提物高(20g/kg)、低(10g/kg)剂量组,在给药30d后,计算小鼠胸腺及脾脏指数,检测小鼠血清MDA含量及SOD活力、肝组织GSH-Px活力、脑组织CAT活力。结果:五指毛桃可显著增大衰老小鼠胸腺、脾脏指数,并不同程度提高衰老小鼠血清SOD活力、肝组织GSH-Px活力、脑组织CAT活力,降低小鼠血清MDA水平。结论:广西产五指毛桃具有一定抗衰老作用,其机制可能与提高机体免疫和抗氧化能力有关。 相似文献
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目的:观察大明胶囊对2型糖尿病大鼠心功能的作用,并探讨其作用机制。方法:用高脂饮食辅以链脲佐菌素(STZ)建立2型糖尿病大鼠模型,将空腹血糖值大于16.7mmol/L的大鼠随机分6组:糖尿病模型组、大明胶囊大剂量(200mg/kg/d)组、中剂量(100mg/kg/d)组、小剂量(50mg/kg/d)组、对照药罗格列酮(0.8mg/kg/d)组。各给药组连续灌胃给药30天后,HE染色和电镜观察大鼠心肌病理形态学改变;RT-PCR方法测定L型Ca2+通道α1cmRNA和K+通道KV4.2mRNA的表达。结果:大明胶囊可改善2型糖尿病所引起的LVSP、+dp/dtmax下降(P0.05),降低LVEDP、-dp/dt-max水平(P0.05),以中剂量作用最明显;形态学显示大明胶囊中剂量减轻了糖尿病大鼠心肌损伤;中剂量还可缩短注射SNP和PE后血压恢复时间以及增加NTS区c-fos免疫反应阳性细胞个数。结论:a.大明胶囊通过影响KV4.2和α1cmRNA的表达改善Q-T间期和P-R间期的延长;b.大明胶囊可通过改善糖尿病大鼠心肌结构的重塑提高2型糖尿病大鼠心脏收缩和舒张功能。 相似文献
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直播密度对富锌水稻锌含量及生理生化特性的影响 总被引：1，自引：0，他引：1
研究了不同直播密度对富锌水稻籽粒中锌含量及茎、叶、籽粒内在生理生化特性(如超氧化物歧化酶(SOD)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化氢酶(CAT)的活性及叶绿素含量等)的影响。试验结果表明,直播密度对富锌水稻籽粒的锌含量有一定影响,随着直播密度的增加,锌含量呈下降趋势,而对各种酶的酶活影响不大。试验还发现,叶中各种酶的活性最高,茎次之,籽粒中最低。从总体来看,富锌水稻以播种量7500-8250 kg/km2为宜,此时富锌水稻植株中各种酶的活性、叶绿素和锌含量都较大。 相似文献
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中国东北北部黑土重金属污染趋势分析 总被引：46，自引：0，他引：46
对中国东北北部 2 2个县市黑土可能发生重金属污染的污染源进行了系统调查 ;对土壤样品中Cd、Pb、Cu、Zn进行检测分析 ,4种重金属的检出范围依次为 0 0 1 3— 2 31mg kg ,1 0 5 2— 5 7 48mg kg ,42 1 3— 2 2 7 1 0mg kg和 9 95— 46 1 8mg kg .结合单污染指数评价法和表底土比较 2种方法进行污染评判 ,结果表明 ,土壤样品中重金属Cd的污染最为明显 ,其污染多集中在中度和重度水平 .对于重金属Pb、Zn和Cu的污染用单污染指数评判没有准确反映污染的真实情况 ,同时说明不同评判方法的精确性有较大的差距 .用表底土比较法对Pb、Cu、Zn 3种重金属进行评判 ,发现其在采样区域的污染水平仍非常明显 ,依次有 5 7 1 %,2 1 0 %和 5 3 3%的表土样品受到这 3种重金属不同程度的污染 .大部分受到污染的土壤样点大多集中在市内工业区附近土壤和城乡结合部菜地 相似文献
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G. R. Sajitha K. T. Augusti Regi Jose 《Indian journal of clinical biochemistry : IJCB》2016,31(3):260-269
Heavy metal pollution is a global public health challenge due to its stable and persistent environmental contamination. Of these lead is considered to be one of the most common ubiquitous and industrial pollutants and at low concentration it exerts extensive damages to the tissues. Daily feeding of lead acetate solution (Dose: 10 mg/kg/day) to normal rats for a month adversely altered the parameters of blood, serum and tissues, viz; RBC, WBC, Hb, ?- ALAD (Delta amino levulinic acid dehydratase), Pb content, lipids, oxidized lipids (TBARS), vitamins C and E and GSH levels and activities of AST, ALT and antioxidant enzymes viz; catalase, GR, Gpx and SOD. In order to study whether antioxidants have any effect to counteract the toxicity of lead we have selected comparatively better active allium fractions for the study viz: polar fraction of garlic (PFG) and polar fraction of onion (PFO). On feeding of these active fractions of garlic and onion oils i.e. their polar fractions and vitamin E (Dose 100 mg/kg/day) separately for a month along with or without lead acetate to rats each nutraceutical and vitamin E counteracted the adverse effects of Pb significantly (p ≤ 0.05). Their effects are in the order of PFG > PFO > Vitamin E. All these results point out that garlic and onion oils contain natural disulfoxide compounds which act as antioxidant and anti toxic to lead compounds. Their comparative differences in action may be due to the presence and position of double bonds and disulfide oxide bonds in their molecules. i.e., in PFG the allyl disulfide oxide group is present and in PFO saturated methyl and propyl groups and unsaturated propenyl group are present in place of allyl groups. The former group confers a better antioxidant activity on PFG, while the latter groups confer a lesser activity on PFO. 相似文献
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K. Rohini M. Surekha Bhat P. S. Srikumar A. Mahesh Kumar 《Indian journal of clinical biochemistry : IJCB》2016,31(3):332-335
The aim of the study was the assessment of hematological parameters in pulmonary tuberculosis patients. Forty patients diagnosed with tuberculosis were recruited from the Institute of Thoracic Medicine on the basis of history, clinical examination, chest radiography, sputum examination and related laboratory parameters and were compared with age and sex matched healthy volunteers (n = 40). Hematological parameters and CRP in tuberculosis patients were determined. The mean values for serum hemoglobin level, RBC count and platelet count in PTB was found to be less (p < 0.001). Erythrocyte sedimentation rate (ESR), plasma C-reactive protein, WBC count in PTB subjects was increased (p < 0.001 for ESR & CRP, p < 0.05 for WBCs) and all were statistically significant. This study demonstrated that serum hemoglobin level, RBC count and platelet count was decreased in tuberculosis patients whereas ESR, CRP and WBC count was increased when compared with healthy controls. 相似文献
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A. S. Thakur G. P. Littaru S. Moesgaard C. Dan sindberg Y. Khan C. M. Singh 《Indian journal of clinical biochemistry : IJCB》2013,28(2):185-188
The study has been undertaken as number of sickle cell patients in Chhattisgarh tribal population is 23.7 %. The Co enzyme Q10 is a strong antioxidant and energy producing compound. The patients were divided into three groups group A homozygous (SS), group B heterozygous (AS) and group C controls for TBARS study. The age group is 10–55 years and 200 mg of CoQ10 was given to A and B groups. The hematological parameters, C reactive protein as well as RBC TBARS level were performed by usual and standard techniques. The results were obtained as 25.37 % increased RBC level in group A and 23.24 % in group B. The increased hemoglobin level was observed as 16.73 % in group A and 10.7 % in group B. In case of WBC it was observed increased 24.38 % in group A and 12.0 % in group B. C-reactive protein was observed 7.8 times decreased in group A and 1.54 times in group B. The RBC TBARS level was also found decreased 48 % in group A and 51 % in group B as compared to group C. During the supplementation of coenzyme Q10 the pain caused by vaso-occlusive events has reduced. This significant increase in hematological parameters as well as decreased C-reactive protein and TBARS level suggest that the Q10 should be included in the diet of sickle cell patients. 相似文献
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几丁质对肿瘤及免疫作用的实验研究 总被引：4，自引：0，他引：4
采用微晶几丁质，观察其对小鼠肿瘤的抑制作用及对免疫功能和细胞毒性的影响。结果显示：经灌胃途径给予剂量为４００ｍｇ／ｋｇ的几丁质制剂，具有延缓小鼠腹水瘤生和作用；对小鼠实体瘤Ｓ１８０生长具有抑制作用，其抑瘤率达３３％。研究还表明，几丁质对小鼠腹腔巨噬细胞的吞噬功能具有明显的促进作用；对人二倍体２ＢＳ细胞和小鼠骨髓瘤ＳＰ２／０细胞地直接 相似文献
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Shalvika Gupta Vijetha Shenoy Belle Ramya Kumbarakeri Rajashekhar Sushma Jogi RV Krishnananda Prabhu 《Indian journal of clinical biochemistry : IJCB》2018,33(4):445-449
Cholinesterases belongs to class hydrolases. There are two types acetylcholinesterase and butyryl cholinesterase. Acetylcholinesterase present in nerve endings and also in the RBC membrane. It helps to maintain the shape and size of RBCs. Any change in shape and size of RBCs may affect the activity of Acetylcholinesterase. Thus this study aimed to estimate RBCs Acetylcholinesterase enzyme activity in various types of anemias and correlate the RBCs Acetylcholinesterase enzyme activity with various hematological indices such as Erythrocyte Sedimentation Rate (ESR), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), Mean Corpuscular Volume (MCV), Red cell Distribution Width (RDW) etc. After obtaining ethical approval from Institutional ethics committee total of 100 samples were collected from Clinical Biochemistry laboratory, Kasturba Medical College, Manipal, Manipal University. 25 were having normal RBC indices, 12 with hemolytic anemia, 26 with microcytic anemia and 26 with macrocytic anemia based on peripheral smear report and RBC indices. Acetylcholinesterase were measured using Ellman’s method. RBC acetylcholinesterase activity was significantly increased in microcytic anemia (58.13 ± 5.4) and macrocytic anemia (76.87 ± 6.7) than normal group (37.62 ± 2.71). Also increased RBC acetylcholinesterase was seen in hemolytic anemia (48.11 ± 5.18) but the increase is not statistically significant. RBC acetylcholinesterase correlated negatively with hemoglobin (r = ?0.356, p = 0.001) and positively with RDW (r = 0.31, p = 0.003). To conclude RBC acetylcholinesterase activity can be used as one of the potential marker for various types of anemia. 相似文献
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Manisha De N. Banerjee G. Talukdar D. K. Bhattacharya 《Indian journal of clinical biochemistry : IJCB》2000,15(1):56-59
The aim of the present work is to understand the effect of low dose of hydroxyurea (HU) therapy on oxidative damage of RBC
membrane in non-transfused Eβ thalassaemia. HU was administered at a dose of 30 mg/kg/day for 90 consecutive days. The percentage
of spectrin and the level of malondialdehyde (MDA), fetal hemoglobin (HbF), hemoglobin (Hb) and packed cell volume (PCV) were
measured. The HbF level was significantly increased after 90 days of HU therapy. MDA level of RBC membrane was decreased.
There was no change in PCV, Hb and spectrin content of RBC membrane after HU therapy for 90 days in Eβ thalassaemia. Increment
of HbF in HU treated Eβ patient may have some role on the correction of oxidative damage of RBC membrane by inhibiting further
degradation of spectrin and by decreasing lipid peroxidation of red cell membrane. 相似文献
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The present study was undertaken to analyze the antioxidant (both enzymic and nonenzymic) activities of leaves of Ocimum sanctum hydroalcoholic extract against cadmium induced damage in albino rats. Oral administration of cadmium as CdCl2 (6.0 mg/kg body weight) led to significant elevation of lipid peroxidation (LPO) levels and significantly decreased Superoxide
Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPx), Reduced Glutathione (GSH) and Vitamin C (Ascorbate) levels.
Administration of Ocimum sanctum extract (100 mg/kg body weight, po) and (200 mg/kg body weight, po) before and after cadmium intoxication showed a significant
decrease in LPO levels and significant increase in SOD, CAT, GPx, GSH and Ascorbate levels. The results suggest that oral
administration of Ocimum sanctum extract provides significant protection against cadmium induced toxicity in Wistar albino rats. 相似文献
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Menon BR Rathi MA Thirumoorthi L Gopalakrishnan VK 《Indian journal of clinical biochemistry : IJCB》2010,25(4):401-404
The study was designed to evaluate the hepatoprotective activity of ethanolic extract of Bacopa monnieri in acute experimental liver injury induced by Nitrobenzene in rats. The extract at the dose of 200 mg/kg body weight was
administered orally once every day for 10 days. The increased serum marker enzymes, Aspartate transaminase, Alanine transaminase
and alkaline phosphatase were restored towards normalization significantly by the extract. Significant increase in SOD, CAT
and GPx was observed in extract treated liver injured experimental rats. Histopathological examination of the liver tissues
supported the hepatoprotection. It is concluded that the ethanolic extract of Bacopa monieri plant possess good hepatoprotective activity. 相似文献
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基于无套利利率模型的台风巨灾债券定价研究 总被引：2，自引：0，他引：2
我国是世界上遭受自然灾害损失最严重的国家之一，应该充分发挥保险业分散巨灾风险和补偿经济损失的作用。巨灾债券作为国外保险发达市场的一项金融创新产品，成功地提高了保险公司对巨灾风险的承保能力。本文利用非寿险精算技术，对我国1990年来损失在1亿元以上的台风损失以及次数分布进行拟舍，确定我国每年台风发生的总损失服从复合泊松-伽玛分布的聚合损失分布模型。随后结合无套利BDT利率期限结构模型以及转移概率参数，来匹配未来利率的变化过程，建立了我国巨灾债券短期利率离散形式的动态变化模型。在此基础上，完成了我国到期保证偿还型台风巨灾债券设计的定价研究。 相似文献