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961.
962.
963.
Shun-zhi Liu Hong Yan Wei-kun Hou Peng Xu Juan Tian Li-fang Tian Bo-feng Zhu Jie Ma She-min Lu 《Journal of Zhejiang University. Science. B》2009,10(8):609-618
Objective: To investigate the relationships between endothelial nitric oxide synthases (eNOS) G894T and 27 bp-variable number tandem repeat (VNTR) gene polymorphisms and osteoporosis in the postmenopausal women of Chinese Han nationality. Methods: In the present study, 281 postmenopausal women from Xi'an urban area in West China were recruited, and divided into osteoporosis, osteopenia, and normal groups according to the diagnostic criteria of osteoporosis proposed by World Health Organization (WHO). The bone mineral density (BMD) values of lumbar vertebrae and left hips were determined by QDR-2000 dual energy X-ray absorptiometry. Blood samples were tested for plasma biochemical indicators including testosterone, estradiol, calcitonin, osteocalcin, and procollagen type I amino-terminal propeptide by enzyme-linked immunosorbent assay (ELISA), tartrate-resistant acid phosphatase by spectrophotometric method, and the content of nitric oxide by Griess method. Genome DNA was extracted from whole blood, and G894T polymorphism of eNOS gene was analyzed by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and 27 bp-VNTR polymorphism of eNOS gene was genotyped by PCR method. Then the relationships between genotypes and biochemical indicators, genotypes and osteoporosis, and haplotypes and osteoporosis were analyzed. Results: The average BMD values of the femoral neck, ward's triangle and lumbar vertebrae 1~4 (L1~L4) in the subjects with T/T genotype in eNOS G894T locus were significantly higher than those in the subjects with G/T and G/G genotypes (P<0.05). The average BMD of the femoral neck in the subjects with a/a genotype of eNOS 27 bp-VNTR locus was evidently higher than that in the subjects with b/b genotype (P<0.05). The plasma testosterone and osteocalcin concentrations in the subjects of eNOS G894T G/T genotype were evidently higher than those in the subjects of other genotypes (P<0.05); the plasma estradiol concentration in the subjects of eNOS 27 bp-VNTR a/a genotype was obviously higher than that in the subjects of b/b genotype (P<0.01). eNOS G/G homozygous frequencies in osteoporosis women, osteopenia women, and normal women were 85.37%, 76.38%, and 83.87%, respectively (P>0.05). 0% osteoporosis woman, 0.79% osteopenia women, and 3.23% normal women were eNOS a/a homozygous (P<0.05). The frequencies of eNOS 27 bp-VNTR a allele were 5.33% in the osteoporosis group, 10.24% in the osteopenia group, and 16.13% in the normal group (P<0.05, odds ratio (OR)=0.29, 95% confidence interval (CI)=0.11~0.77), suggesting that a/a genotype and a allele might have protective effects on osteoporosis. The haplotype analysis showed that G-b was 87.7% (214/244) in the osteoporosis group (P<0.05, OR=2AS, 95% CI=1.18~5.18). G-a was 5.3% (13/244) in the osteoporosis group (P<0.05, OR=0.29, 95% CI=0.11~0.77). G-b was a risk factor for osteoporosis, and G-a a protective factor. Conclusion: eNOS G894T G/T genotype influenced the plasma testosterone and osteocalcin concentrations, and T/T genotype influenced BMD. eNOS 27 bp-VNTR a/a genotype increased plasma estradiol concentration to have a protective effect on osteoporosis. 相似文献
964.
965.
This paper empirically investigates the economies of scale and economies of scope for the Chinese research universities by employing the flexible fixed cost quadratic (FFCQ) function. The empirical results show that both economies of scale and economies of scope exist in the Chinese higher education system and support the commonly belief that universities as multi-product organizations. 相似文献
966.
情报研究是一项极其复杂的知识活动,对情报价值的定量化研究具有一定的难度,本文较为全面地阐述了国内外情报价值定量化研究的现状和热点,重点对情报价值量化理论与量化方法进行全面综述. 相似文献
967.
摘要:通过绘制科学计量学研究前沿知识图谱,界定出七大国际科学计量学研究前沿领域:1、引文理论、科学交流、基础科研评价;2、科学知识图谱理论与方法、网络计量学;3、科学技术合作;4、科学计量学经典概率分布、文献计量学定律、信息计量学;5、科学计量学指标与国家科研绩效评价;6、知识的新产品--科技动力学;7、科学与技术的关系。其中前沿领域1、2、4属于理论与方法研究领域,而前沿领域3、5、6、7属于应用研究领域。经过比较发现科学计量学更加侧重于理论与方法研究。 相似文献
968.
图书馆产业运作模式探讨 总被引:1,自引:0,他引:1
从可持续发展的角度,论述了图书馆开展产业运作的必要性以及产业运作的模式和需要处理好的几种关系。 相似文献
969.
970.
为了使防盗报警系统具有智能化和增加安全性,该系统利用凌阳16位单片机SPCE061A芯片对特定人短语音具有较好的语音识别能力,并且采用反混叠滤波、预加重和动态时间规整技术,使系统具有防干扰能力强,成本低,语音识别好等优点,从大量实验知道,该系统防盗报警的准确率和可靠性都较好。 相似文献