Single cell kinase signaling assay using pinched flow coupled droplet microfluidics     

Ramesh Ramji  Ming Wang  Ali Asgar S. Bhagat  Daniel Tan Shao Weng  Nitish V. Thakor  Chwee Teck Lim  Chia-Hung Chen 《Biomicrofluidics》2014,8(3)

Droplet-based microfluidics has shown potential in high throughput single cell assays by encapsulating individual cells in water-in-oil emulsions. Ordering cells in a micro-channel is necessary to encapsulate individual cells into droplets further enhancing the assay efficiency. This is typically limited due to the difficulty of preparing high-density cell solutions and maintaining them without cell aggregation in long channels (>5 cm). In this study, we developed a short pinched flow channel (5 mm) to separate cell aggregates and to form a uniform cell distribution in a droplet-generating platform that encapsulated single cells with >55% encapsulation efficiency beating Poisson encapsulation statistics. Using this platform and commercially available Sox substrates (8-hydroxy-5-(N,N-dimethylsulfonamido)-2-methylquinoline), we have demonstrated a high throughput dynamic single cell signaling assay to measure the activity of receptor tyrosine kinases (RTKs) in lung cancer cells triggered by cell surface ligand binding. The phosphorylation of the substrates resulted in fluorescent emission, showing a sigmoidal increase over a 12 h period. The result exhibited a heterogeneous signaling rate in individual cells and showed various levels of drug resistance when treated with the tyrosine kinase inhibitor, gefitinib.  相似文献   

Information Needs: Developing Personas of Humanities Scholars     

Mohammad Khaled Al-Shboul 《The Journal of Academic Librarianship》2014,40(5):500-509

A persona represents a group of target users that share common behavioural characteristics. The personas method, an approach to systems design, has been receiving significant attention from practitioners. However, only anecdotal evidence currently exists for the effectiveness of personas and there have been criticisms about its validity as a scientific approach to research. This paper attempts to demonstrate how incorporating personas may lead to better understand the information needs of humanities scholars. Humanities scholars in an advanced ICT environment in the Hashemite Kingdom of Jordan are sampled as a case. Previous studies show that the humanities scholars have a significant negative relation with ICT use; they are using it less than scientists and social scientists, and they demonstrate a significantly higher use of library facilities than other academics. There is also a lack of up-to-date international research on the humanities scholars' information needs that takes into account recent rapid increases in the availability of ICT infrastructure, especially the Internet. As such, the objective of this study is to understand the information needs of humanities scholars and the effect of the electronic environment on their information seeking behaviour using personas. This study is conducted within a conceptual framework based on an integration of existing models of information-seeking behaviour, along with additional new elements representing the information context environment, such as languages, decision to seek and format of information resources. The four personas that were uncovered in this study may be able to effectively communicate the actual information needs of the humanities scholars through the personal narrative, name, and face, which continuously will remind the academic library of what their users really want and need from their services. This study also lays the foundation for future research by identifying variables of interest, and building construct validity through the themes of information needs that emerged.  相似文献   

Defending recommender systems by influence analysis     

Mohammad Amin Morid  Mehdi Shajari  Ali Reza Hashemi 《Information Retrieval》2014,17(2):137-152

Collaborative filtering (CF) is a popular method for personalizing product recommendations for e-commerce applications. In order to recommend a product to a user and predict that user’s preference, CF utilizes product evaluation ratings of like-minded users. The process of finding like-minded users forms a social network among all users and each link between two users represents an implicit connection between them. Users having more connections with others are the most influential users. Attacking recommender systems is a new issue for these systems. Here, an attacker tries to manipulate a recommender system in order to change the recommendation output according to her wish. If an attacker succeeds, her profile is used over and over again by the recommender system, making her an influential user. In this study, we applied the established attack detection methods to the influential users, instead of the whole user set, to improve their attack detection performance. Experiments were conducted using the same settings previously used to test the established methods. The results showed that the proposed influence-based method had better detection performance and improved the stability of a recommender system for most attack scenarios. It performed considerably better than established detection methods for attacks that inserted low numbers of attack profiles (20–25 %).  相似文献   

Exploring the uses of mobile phones to support informal learning     

Ieda M. Santos  Nagla Ali 《Education and Information Technologies》2012,17(2):187-203

This paper explores how a group of undergraduate students in the United Arab Emirates (UAE) use their mobile phones to perform informal learning activities related to the content of their courses outside the classroom. The paper also addresses the usefulness of informal learning activities to support students’ learning. The study adopts an exploratory case study design and uses multiple methods of data collection including questionnaires, interviews and diary entries. Main findings suggest that students performed informal learning activities mostly from home, interacting mainly with classmates. It also shows that students were in control of their informal learning activities without teachers’ input. However, it was found that students used only a limited number of applications but these were considered useful to their learning. The paper contributes to a discussion of the implications of training and instructor support to help students to take more advantage of mobile phone applications to support informal learning. Recommendations for further research are discussed.  相似文献   

Understanding the rise of e-participation in non-democracies: Domestic and international factors     

Joachim Åström  Martin Karlsson  Jonas Linde  Ali Pirannejad 《Government Information Quarterly》2012

While it has often been suggested that information and communication technologies (ICTs) provide an important means of increasing citizen participation (which is at the core of democratic government), few commentators have expected non-democracies to create online environments in which citizens can take an active part in political processes. In recent years, however, some non-democracies have begun to outperform countries with long-standing democratic traditions in terms of e-participation development. According to the 2010 United Nations (UN) e-government survey, Bahrain outranks France, Kazakhstan beats Sweden and Malaysia ranks higher than Germany. This article sets out to understand the recent rise of e-participation initiatives in non-democracies. Drawing on comparative longitudinal data from the UN e-government surveys, we tested the assertion that international drivers of change are competing with the dominant focus on domestic factors, especially in the non-democratic world, and are influencing the patterns of reform. The empirical analysis demonstrated important differences between the drivers of change in democratic and non-democratic countries and found economic globalization to be the strongest predictor of e-participation initiatives in non-democratic countries. In conclusion, we argue that economic globalization alters the context of e-participation and necessitates a re-examination of many of its premises and tenets.  相似文献   

中国足协处罚权研究          下载免费PDF全文

邓小兵  王阿丽 《河北体育学院学报》2021,35(6):36-43

中国足协的处罚权是其参与体育治理的重要手段,相较于法定性强、形态有限的行政处罚, 更具专业性和针对性。为了保障其良性运转,维护相对人的诉讼权利,司法权的适度介入已成为理论界的共识。但我国传统的行政主体理论不能回应这种社会公权力发展的现实需要,并对其作出合理解释。因此,亟需重构我国的行政主体理论,赋予中国足协行政主体地位,构建司法审查规则,即法院审查足协处罚权时应当遵循仲裁优先原则、穷尽内部救济原则、法律审查原则;在审查范围上,除了审查足协处罚行为,还应当附带审查处罚规则。  相似文献   

21st Century experiences in the development of school-based management policy and practices in Indonesia     

Bandur  Agustinus  Hamsal  Mohammad  Furinto  Asnan 《Educational Research for Policy and Practice》2022,21(1):85-107

Educational Research for Policy and Practice - Since 2001, Indonesian schools have implemented a mandatory school-based management (SBM) policy for better quality education in general and more...  相似文献   

Combining management training and technical training in an international setting     

William Fisher  Ali Kamel Ali 《Performance Improvement》1992,31(9):27-31

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Interactive cube for effective demonstration of virtual periodic table     

Ali  Numan  Ullah  Sehat  Raees  Muhammad 《Education and Information Technologies》2022,27(2):1635-1654

Education and Information Technologies - Virtual Periodic Table (VPT) is an effective software tool to present elements in a conceivable three-dimensional (3D) mode. VPT is used to assist users in...  相似文献   

Multimodal learning analytics—In-between student privacy and encroachment: A systematic review     

Paul Prinsloo  Sharon Slade  Mohammad Khalil 《British journal of educational technology : journal of the Council for Educational Technology》2023,54(6):1566-1586

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