Synthesis of Gold Nanoparticles Coated with Aspartic Acid and Their Conjugation with FVIII Protein and FVIII Antibody     

Maryam Farahnak Zarabi  Ali Farhangi  Samaneh Khademi Mazdeh  Zahra Ansarian  Davod Zare  Mohammad Reza Mehrabi  Azim Akbarzadeh 《Indian journal of clinical biochemistry : IJCB》2014,29(2):154-160

Carboxylate-modified gold nanoparticles (GNPs) were synthesized in a simple one-step process based on the reduction of tetrachloroauric acid by aspartic acid in water. GNPs were identified by UV–Vis spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy. Conjugation of protein molecules with functionalized nanoparticles was performed through electrostatic interaction. The GNP–protein conjugates were characterized by gel electrophoresis. The interaction between functionalized GNPs and protein molecules lead to conformational transition of protein structure after conjugation of protein with GNPs. This process was investigated by fluorescence spectroscopy and circular dichroism spectroscopy.  相似文献   

Immunoisolated Transplantation of Purified Langerhans Islet Cells in Testis Cortex of Male Rats for Treatment of Streptozotocin Induced Diabetes Mellitus     

Ali Farhangi  Dariush Norouzian  Mohammad Reza Mehrabi  Mohsen Chiani  Zahra Saffari  Maryam Farahnak  Azim Akbarzadeh 《Indian journal of clinical biochemistry : IJCB》2014,29(4):406-417

The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult Wistar rats via comparison of changes in body weight, consumption of food, volume of water, urine and levels of glucose, insulin and C-peptide in serum, between normal and diabetic rats. Intra-venous injection of 60 mg/kg dose of streptozotocin in 250–300 g (75–90 days) adult Wistar rats makes pancreas swell and causes degeneration in Langerhans islet β-cells and induces experimental diabetes mellitus in 2–4 days. For a microscopic study of degeneration of Langerhans islet β-cells of diabetic rats, biopsy from pancreas tissue of diabetic and normal rats, staining and comparison between them, were done. In this process, after collagenase digestion of pancreas, islets were isolated, dissociated and identified by dithizone method and then with enzymatic procedure by DNase and trypsin, the islet cells changed into single cells and β-cells were identified by immune fluorescence method and then assayed by flow-cytometer. Donor tissue in each step of work was prepared from 38 adult male Wistar rats weighted 250–300 g (75–90 days). Transplantation was performed in rats after 2–4 weeks of diabetes induction. In this study, the levels of insulin, C-peptide and glucose in diabetic rats reached to normal range as compared to un-diabetic rats in 20 days after transplantation of islet cells. Transplantation was performed under the cortex of testis as immunoisolated place for islet cells transplantation.  相似文献   

Different effects of L-arginine on morphine tolerance in sham and ovariectomized female mice     

Karami R  Hosseini M  Khodabandehloo F  Khatami L  Taiarani Z 《Journal of Zhejiang University. Science. B》2011,12(12):1016-1023

Objective  

The roles of gonadal hormones and nitric oxide (NO) on the analgesic effects of morphine, tolerance to morphine, and their interactions have been widely investigated. In the present study, the effect of l-arginine (an NO precursor) on morphine tolerance in sham and ovariectomized (OVX) female mice was investigated.  相似文献   

Medical education and COVID-19 pandemic: a crisis management model towards an evolutionary pathway     

Karimian  Zahra  Farrokhi  Majid Reza  Moghadami  Mohsen  Zarifsanaiey  Nahid  Mehrabi  Manoosh  Khojasteh  Laleh  Salehi  Nasim 《Education and Information Technologies》2022,27(3):3299-3320

Education and Information Technologies - The COVID-19 crisis has had a profound effect on higher education, especially medical education due to its sensitive nature, dealing with people’s...  相似文献   

The effects of teaching self-determination skills on the externalizing behaviors of students with emotional behavioral disorders     

Zahra Mansurnejad  Amir Ghamarani  Ahmad Yarmmohamadiyan 《Emotional and Behavioural Difficulties》2019,24(2):196-203

The purpose of the present study was to examine the effects of teaching self- determination skills on the externalizing behaviors of students with emotional and behavioral disorders. The Research method was quasi experimental with pre and post- test and control group. To achieve our goals, thirty students who received high scores in the Child Behavior Checklist (CBCL) were selected and randomly assigned to either the experimental group (N = 15) or the control group (N = 15). Students in the experimental group participated in 16 sessions (1 session per week) receiving Self determination curriculum (Field and Hoffman’s model) intervention, and the control group did not receive any training. The Child Behavior Checklist (CBCL) by participants was completed before and after intervention. MANOVA was used for data analysis. The findings showed that the effects of teaching self- determination skills on decreasing the externalizing behaviors of students with emotional and behavioral disorders was significant. The emerged finding can have clinical applications in prevention and treatment of student’s behavioral disorders. Implications and future directions for research and practice are discussed.  相似文献   

Scallywags – an evaluation of a service targeting conduct disorders at school and at home     

M.A. Broadhead  A. Hockaday  M. Zahra  P.J. Francis  C. Crichton 《Educational Psychology in Practice》2009,25(2):167-179

Conduct disorder (CD) is one of the most common childhood psychiatric disorders. Some research has focused on reducing conduct difficulties through parent training programmes. However, there has been limited research focusing on early intervention services that deal with emotional and/or conduct problems in a community setting. The aim of this study was to evaluate the overall effectiveness of the Scallywags service, a multi‐component, early intervention scheme, offering support in the educational and home setting. A total of 411 children, aged 3 to 7 years, was included in this study. Two behaviour inventories were used as the outcome measures. Parents and teachers completed these pre, post, and six months after the intervention. Results demonstrate significant decreases in child conduct problems after the six months intervention, with this change maintained six months later. This study adds to growing research illustrating the importance of multi‐component, early intervention schemes to reduce child conduct problems.  相似文献   

Reduction of the non-uniform illumination using nonlocal variational models for document image analysis     

Fatim Zahra Ait Bella  Mohammed El Rhabi  Abdelilah Hakim  Amine Laghrib 《Journal of The Franklin Institute》2018,355(16):8225-8244

In this paper, we investigate two new reflectance and illumination decomposition models based on a nonlocal partial differential equation (PDE) applied to text images. Taking into consideration the higher regularity level of the illumination compared to the reflectance, we propose a nonlocal PDE which deals with repetitive structures and textures that characterize the text image much better compared to the classical local PDEs. The aim of this approach is to use the repetitive features of the reflectance to efficiently extract it from the non-uniform illumination. This idea is motivated by extending the range of application of the nonlocal operators to such a problem. Numerical experiments on both grayscale and color text images show the performance and strength of the proposed nonlocal PDE.  相似文献   

Hands-on engagement online: using a randomised control trial to estimate the impact of an at-home lab kit on student attitudes and achievement in a MOOC     

Jennifer DeBoer  Casey Haney  S. Zahra Atiq  Casey Smith  David Cox 《European Journal of Engineering Education》2019,44(1-2):234-252

ABSTRACT

A shortcoming of Massive Open Online Courses (MOOCs) is the lack of substantive integration of hands-on activities into online classrooms. We conducted a randomised control trial (RCT) in a MOOC where we sent the treatment group at-home lab kits with which they could perform experiments in their own homes. In our intervention research, we found that, at the end of the course, students in the treatment group had significantly higher exam scores and levels of self-efficacy to perform in the topic area than their control group counterparts. Students’ growth in self-concept was not significantly different. Findings have implications for distance courses in engineering and other science, technology, engineering, and math areas that traditionally have laboratory components. Furthermore, our findings illustrate the potential amplifying effect of this learning environment configuration for engineering instructors. Finally, this study illustrates how the dispersed nature of MOOCs facilitates RCTs and a recursive process linking theory and practice.  相似文献   

“Throwing salt on wounds”: Covid-19 and a curriculum of embodiment     

Kasamali  Zahra 《Prospects》2021,51(1-3):103-116

PROSPECTS - The Covid-19 pandemic certainly amplifies the extent to which curriculum is adaptable, responsive, and proactive. These vulnerabilities, while daunting, can perhaps be welcomed as an...  相似文献   

茉莉酸对油菜(Brassica napus L.)受镉毒害的缓解作用（英文）     

Essa ALI  Nazim HUSSAIN  Imran Haider SHAMSI  Zahra JABEEN  Muzammil Hussain SIDDIQUI  Li-xi JIANG 《Journal of Zhejiang University. Science. B》2018,(2)

目的:本研究目的在于了解:(1)喷施外源茉莉酸对受到镉胁迫油菜的作用;(2)是否茉莉酸能够通过增强气体交换,从而保护受到氧化胁迫的地上部分组织的叶绿体,进而通过减少镉的吸收来维持离子平衡;(3)是否通过喷施茉莉酸来对具有减缓镉毒害效应的抗氧化酶的活性进行调节。创新点:茉莉酸能够调节响应胁迫的抗氧化酶的活性,从而通过保护叶绿体免受活性氧(ROS)伤害而提高光合产物的能力,最大限度地缓解油菜植株受到的镉毒害。方法:(1)叶片气体交换;(2)叶片光合色素分析;(3)丙二醛与抗氧化酶活性分析;(4)营养成分分析;(5)透射电镜亚细胞水平观察。结论:茉莉酸对于植物受镉毒害的缓解作用的机理在于减少叶片中镉的积累,从而减轻氧化胁迫过程中产生的ROS对于膜系统的损害程度。  相似文献