Molecular Characterization of Iranian Patients with Possible Familial Hypercholesterolemia     

Farrokhi E  Shayesteh F  Asadi Mobarakeh S  Roghani Dehkordi F  Ghatreh Samani K  Hashemzadeh Chaleshtori M 《Indian journal of clinical biochemistry : IJCB》2011,26(3):244-248

Familial hypercholesterolemia (FH) is an autosomal dominant disorder of lipoprotein metabolism caused mainly by mutations in the low-density lipoprotein receptor (LDLR) and apolipoprotein B 100 (APOB) genes. Until now, the molecular basis of FH has been demonstrated in detail in many populations, but there is still very limited Molecular data concerning FH in Iran. The aim of this study was to characterize the LDLR and APOB gene mutations in an Iranian population. A total of 30 non-related Iranian possible FH subjects were studied. Diagnosis of FH was based on the Dutch Lipid Clinic Network diagnostic criteria. All samples were initially tested for three common APOB gene mutations including R3500Q, R3500 W and R3531C using PCR-RFLP assay. Subsequently, promoter and coding region of the LDLR gene was screened by PCR-SSCP analysis and positive results were confirmed by DNA sequencing. Four previously reported polymorphisms 1413G > A, 1725C > T, 1773T > C and 2140 + 5G > A were found in ~17% (5/30) of population studied. Moreover, no variation was found in APOB gene. Our data indicated that LDLR and APOB gene mutations have not contribution to possible FH in Iranian population studied here. However, we examined three common APOB mutations and LDLR in only 30 patients, and to determine the role of these genes in developing FH in Iran, more FH samples and populations needed to be investigated for the mutations of the related genes.  相似文献   

Medical education and COVID-19 pandemic: a crisis management model towards an evolutionary pathway     

Karimian  Zahra  Farrokhi  Majid Reza  Moghadami  Mohsen  Zarifsanaiey  Nahid  Mehrabi  Manoosh  Khojasteh  Laleh  Salehi  Nasim 《Education and Information Technologies》2022,27(3):3299-3320

Education and Information Technologies - The COVID-19 crisis has had a profound effect on higher education, especially medical education due to its sensitive nature, dealing with people’s...  相似文献   

Nonlinear model-predictive control with disturbance rejection property using adaptive neural networks     

Bahareh Vatankhah  Mohammad Farrokhi 《Journal of The Franklin Institute》2017,354(13):5201-5220

In this paper, a method is proposed to reject disturbances in the model predictive control (MPC) strategy. In addition, uncertainties in the system parameters (i.e., internal disturbances) are considered as well. To achieve these goals, adaptive neural networks are designed as the predictor model and as the nonlinear disturbance observer, respectively. The disturbances are rejected via the optimization problem of the MPC. Stability of the closed-loop system is studied based on the Input-to-State Stability method. The proposed method is applied to the pH neutralization process and CSTR system and its effectiveness in optimal rejection of the disturbances and satisfying the system constrains is compared with the feed-forward control method.  相似文献   

Comparison of the effects of acute and chronic psychological stress on metabolic features in rats     

Fatemeh Rostamkhani  Homeira Zardooz  Saleh Zahediasl  Babak Farrokhi 《Journal of Zhejiang University. Science. B》2012,13(11):904-912

This study was aimed to compare the effects of acute and chronic psychological stress on metabolic factors. Forty-two male Wistar rats were divided into control and stressed groups. Stress was applied by a communication box acutely (1 d) and chronically (15 and 30 d). Blood sampling was carried out by retro-orbital-puncture method. The plasma levels of glucose, cholesterol, triglyceride, insulin, and corticosterone were measured. In addition, feed and water intake, latency to eat and drink, adrenal and body weights were determined. Acute and chronic psychological stress did not significantly change basal plasma corticosterone levels. However, immediately (1 min) after acute exposure to stress, plasma corticosterone level increased compared to that before stress exposure. Acute stress increased plasma insulin levels significantly. Fifteen days of stress exposure resulted in plasma glucose increase. Chronic stress significantly increased feed intake, latency to eat, and adrenal weight compared to acute stress. The body weights of both control and stressed groups increased markedly during the experiment. Homeostasis model assessment of insulin resistance (HOMA-IR) index did not change significantly in the stressed group. In conclusion, application of acute and chronic psychological stress leads to different metabolic and/or behavioral changes but the metabolic changes resulting from acute exposure to stress seem to be more pronounced.  相似文献