作物AFLP差异片段的回收与克隆     

张宁洁 《毕节学院学报》2009,27(4)

通过高低油酸花生的种植、DNA的提取、双酶切、连接、预扩增、再扩增、制胶及差异片段的回收、纯化、克隆等一系列操作,摸索出一套AFLP分子标记下的分子克隆技术,适合于花生,也适合于其他经济作物.  相似文献   

最小支撑树的DNA凝胶电泳算法     

江智兰  周金凤 《人天科学研究》2013,(3):58-60

DNA计算是解决困难问题的一种很重要的方法。应用DNA计算解决图论中的最小支撑树问题。利用DNA的热力学特性,根据边的权长不同,给它们设计不同溶解温度的DNA链。根据温度的不同,电泳时DNA分子的形状不同,电泳的速度也不同,从而根据电泳速度分离出最小支撑树的所有边。在这里给出了5个顶点的赋权图为例来求它的最小支撑树,说明了该方法的简便性。  相似文献   

生物传感器技术与DNA计算结合的应用研究     

闾素红  余华  惠向晖 《科技通报》2013,(10)

与目前压电基因传感器技术和DNA计算理论相结合,设计了一种基于压电基因传感器DNA计算的方法,并把此方法应用到可满足性问题的解决上,详细论述了计算的过程,并编写程序模拟和验证算法的可行性。与以前的DNA计算方法相比,该方法具有运算和检测解的同时性,计算的准确性和并行性以及计算过程的连续性等优点,说明将传感器技术和DNA计算相结合的生物高科技技术,在DNA计算及其应用中有着巨大的使用价值。  相似文献   

人类新基因HBVDNAPTP1结合蛋白的生物信息学分析     

伦永志  刘为国  朱莹  张国元  毛小强  司红敏  王琳 《大连大学学报》2006,27(6):80-83

[目的]应用生物信息学分析人类新基因乙型肝炎病毒(HBV)DNA聚合酶(Polym erase)反式调节蛋白(HBVDNAPTP1)结合蛋白(HBVDNAPTP1BP).[方法]利用生物信息学技术分析HBVDNAPTP1BP基因的染色体定位与组织表达,以及编码蛋白的化学物理性质与结构特征.[结果]HBVDNAPTP1BP基因染色体定位于1号染色体短臂3区5带1亚带,可在组织中低表达,但在多个组织中无表达.HBVD-NAPTP1BP的相对分子量为11 905.6,理论pI为7.72,不同条件下的消光系数为14 230 M-1cm-1或13 980M-1cm-1(280 nm),在体外哺乳动物网状细胞中的半寿期为30 h,并且无卷曲螺旋区域,但具有3个较强的疏水区域.HBVDNAPTP1BP无特殊二级结构,仅有1个蛋白激酶C磷酸化位点,不具有跨膜螺旋结构,无信号肽序列,定位于细胞核中.[结论]应用生物信息学对HBVDNAPTP1BP进行了分析,为进一步研究其生物学功能及其在乙型肝炎、肝细胞癌中的作用机制提供了依据和线索.  相似文献   

DNA双螺旋结构动态演示仪设计     

王宇  乔怡涛  张楠  黄志东 《辽宁科技学院学报》2019,21(2):13-14

针对现有DNA教具缺陷,分析DNA螺旋结构特征,提出一种DNA双螺旋结构动态演示仪。运用3D打印技术制作形状特殊的零部件,引入特殊的尼龙线配合球头副辅助DNA运动,最终制作出DNA双螺旋结构动态演示仪。经实际授课使用,证明该演示仪能够辅助提高教学效果。  相似文献   

Isolation and identification of Sclerotinia stem rot causal pathogen in Arabidopsis thaliana     

Wang AR  Lin WW  Chen XT  Lu GD  Zhou J  Wang ZH 《Journal of Zhejiang University. Science. B》2008,9(10):818-822

A new stem rot disease is found to occur naturally on Arabidopsis plants in greenhouses of Fuzhou, China. In order to identify its pathogen, we conducted a series of fimgal isolation and purification, plant reinoculation, and ascus and ascospore induction from the sclerotia. The isolate caused typical water-soaked lesions after reinoeulation and produced sclerotia both on Arabidopsis plants and culture medium plates, and the sclerotia could be induced to produce discal apothecia and 8 binucleate ascospores per ascus. These disease symptom and fungal morphology data revealed that the fungus Sclerotinia sclerotiorum (Lib.) de Bary was the pathogen for Arabidopsis stem rot. To confirm this, we further amplified its large subunit ribosomal DNA (LSU rDNA) by polymerase chain reaction (PCR), and compared the sequence with the known LSU rDNA sequences in GenBank. The results show that the sequence shares the highest identities with the LSU rDNAs of different S. sclerotiorum strains. Taking all these data together, we concluded that the fungus that caused the Arabidopsis stem rot is S. sclerotiorum (Lib.) de Bary. This is the first report that Arabidopsis is naturally infected by S. sclerotiorum.  相似文献   

基于三链DNA结构的全错位排列问题算法     

孙侠  殷志祥  赵前进  许峰 《滁州学院学报》2012,14(2):18-20

目前,一种新型的DNA计算模型——三链DNA计算模式正越来越受到人们的关注。已经证实,DNA单链能在RecA蛋白的介导下与同源的双链DNA匹配成稳定的三链DNA结构,利用此三链核酸提取目的DNA序列是完全可行的。文章提出了全错位排列问题的基于三链DNA的计算模型,由于表示可能解的链都是双链,彼此不会错配,也不会形成发夹结构,这样就大大降低了编码复杂度和计算错误率。  相似文献   

Fungal diversity in adult date palm (Phoenix dactylifera L.) revealed by culture-dependent and culture-independent approaches     

Ines Ben Chobba  Amine Elleuch  Imen Ayadi  Lamia Khannous  Ahmed Namsi  Frederique Cerqueira  Noureddine Drira  Néji Gharsallah  Tatiana Vallaeys 《Journal of Zhejiang University. Science. B》2013,14(12):1084-1099

Endophytic flora plays a vital role in the colonization and survival of host plants, especially in harsh environments, such as arid regions. This flora may, however, contain pathogenic species responsible for various troublesome host diseases. The present study is aimed at investigating the diversity of both cultivable and non-cultivable endophytic fungal floras in the internal tissues (roots and leaves) of Tunisian date palm trees (Phoenix dactylifera). Accordingly, 13 isolates from both root and leaf samples, exhibiting distinct colony morphology, were selected from potato dextrose agar (PDA) medium and identified by a sequence match search wherein their 18S–28S internal transcribed spacer (ITS) sequences were compared to those available in public databases. These findings revealed that the cultivable root and leaf isolates fell into two groups, namely Nectriaceae and Pleosporaceae. Additionally, total DNA from palm roots and leaves was further extracted and ITS fragments were amplified. Restriction fragment length polymorphism (RFLP) analysis of the ITS from 200 fungal clones (leaves: 100; roots: 100) using HaeIII restriction enzyme revealed 13 distinct patterns that were further sequenced and led to the identification of Alternaria, Cladosporium, Davidiella (Cladosporium teleomorph), Pythium, Curvularia, and uncharacterized fungal endophytes. Both approaches confirmed that while the roots were predominantly colonized by Fusaria (members of the Nectriaceae family), the leaves were essentially colonized by Alternaria (members of the Pleosporaceae family). Overall, the findings of the present study constitute, to the authors’ knowledge, the first extensive report on the diversity of endophytic fungal flora associated with date palm trees (P. dactylifera).  相似文献   

DNA序列数形表示的研究     

胡金玉  胡应平 《科技广场》2008,22(1):42-43

DNA序列的数形表示是用数学方法和计算机处理方法分析生物分子序列首先要解决的问题。序列表示的优劣会对最终分析结果有直接的影响。本文介绍了现有几种DNA序列数形表示方法。  相似文献   

DNA分子标记技术在水生动物遗传多样性研究中的应用   总被引：1，自引：0，他引：1  

王忠华  董西征  钱国英 《科技通报》2008,24(5)

简要阐述了几种常见DNA分子标记技术,如限制性片断长度多态性(restriction fragment length polymorphism,RFLP)、随机扩增多态性DNA(random amplified polymorphie DNA,RAPD)、扩增片断长度多态性(amplified fragment length polymorphism,AFLP)、简单重复序列或微卫星标记(simpie sequencerepeat,SSR)、内部简单重复序列(inter-simple sequence repeat,ISSR)和单核苷酸多态性(single nucleotidepelymorphisms,SNP)等的基本原理、技术上的优缺点及其在水生动物遗传多样性研究中的应用,包括遗传多样性的鉴定、品种亲缘关系与分类的研究和种质资源的鉴定等.最后,对DNA分子标记技术在水生动物遗传多样性应用中的存在问题进行了简单的讨论.  相似文献