核糖对大强度耐力训练大鼠血清酶活性和血清尿素、血糖、肌肝糖原含量的影响   总被引：1，自引：0，他引：1  

熊正英  曲洪刚  刘海斌 《天津体育学院学报》2006,21(5):420-422

目的：探讨核糖对大强度耐力训练大鼠血清生化指标影响的机理．为核糖作为运动补剂提供试验依据。方法：选取24只大鼠．随机分为安静组、训练组和核糖加药组，训练组与训练加药组参照洪平等的训练模型进行3周的训练，最后一次训练进行一次性力竭运动，力竭后取血并进行样本处理。内容：利用ACE全自动生化分析仪，通过测定与运动训练相关的血清酶，血清尿素（BU）、血糖（Glu）浓度和肌肝糖原含量，研究了核糖对重要脏器的保护作用以及对糖、蛋白质代谢、大鼠跑台至力竭时间的影响。结论：核糖可保护重要器官免受运动的损伤；可促进运动中糖异生作用．有利于维持运动过程中血糖水平的稳定：可明显延缓大鼠运动疲劳的发生，使大鼠运动能力明显提高。  相似文献   

Isolation and physiological characteristics of a premature senescence mutant in rice(Oryza sativa L.)     

李付振  金松恒  胡国成  付亚萍  斯华敏  蒋德安  孙宗修 《Journal of Zhejiang University. Science. B》2005,(8)

INTRODUCTION Leaf senescence is a kind of programmed cell death (PCD) in plants that is highly controlled by a genetically programmed process involving degrada-tion of cellular structures, decline of photosynthesis massive generation of active oxygen species (AOS) and mobilization of nutrients (Nam, 1997; Lim et al.,2003). Many environmental factors have been shown to play a role in causing the photosynthetic decline to lead to premature senescence. Leaves exposed to ultraviolet radia…  相似文献   

Isolation and physiological characteristics of a premature senescence mutant in rice （Oryza sativa L.）     

Li FZ  Jin SH  Hu GC  Fu YP  Si HM  Jiang DA  Sun ZX 《Journal of Zhejiang University. Science. B》2005,6(8):803-811

A rice pse(t) (premature senescence, tentatively) mutant line, was isolated from 4500 independent T-DNA inserted transgenic lines. The symptoms of premature senescence appeared more severely than those of the control plants (Zhonghua 11,japonica) at the last development stage. To characterize the mutant and provide basic information on the candidate genes by mapping to a physical region of 220-kb, experiments were carried out in two phytotrons under controlled temperature of 24 ℃ and28 ℃, respectively. The content of chlorophyll, soluble protein and MDA (malondialdehyde), net photosynthesis, the antioxidant enzyme activities of SOD (superoxide dismuase) (EC 1.15.1.1) and POD (peroxidase) (EC 1.11.1.7) and the peptidase activities of leaves were measured from top to bottom according to the leaf positions at the flowering stage. Compared with the control plant,the mutant showed the following characteristics: (1) Higher net photosynthesis rate (Pn) appeared in the 1 st and 2nd leaves, contents of chlorophyll and soluble protein were also higher in the 1 st leaf; (2) The activities of SOD, POD and peptidase were higher according to the leaf position from top to bottom; (3) The symptom of premature senescence was accelerated in the mutant at 28℃treatment. The MDA content and the SOD and POD activities between the 24 ℃ and 28 ℃ treatment mutants were not significantly different. Content of chlorophyll and soluble protein of leaves mutant decreased rapidly at 28 ℃ treatment. The results show that pse(t) is sensitive to high temperature. The probable function of PSE(T) is discussed.  相似文献   

Antioxidant activity ofcurculigo orchioides in carbon tetrachloride—induced hepatopathy in rats     

M. R. Venukumar  M. S. Latha 《Indian journal of clinical biochemistry : IJCB》2002,17(2):80-87

In this study antioxidant activity of methanol extract of rhizomes ofCurculigo orchioides (MEC) was investigated using carbon tetrachloride (CCl₄)-intoxicated rat liver as the experimental model. The hepatotoxic rats were administered MEC for 90 days (daily, orally at the dose of 70 mg per kg body weight). Lipid peroxidation (LPO) in CCl₄-intoxicated rats was evidenced by a marked increment in the levels of thiobarbituric acid reactive substances (TBARS) and diene conjugates (CD), and also a distinct diminution in glutathione (GSH) content in the liver. In CCl₄+MEC—treated rats these biochemical parameters attained an almost normal level. The decreased activity of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GRD) in CCl₄—intoxicated rats, and its retrieval towards near normalcy in CCl₄+MEC—administered rats revealed the efficacy of MEC in combating oxidative stress due to hepatic damage. Elevated level of glutathione transferase(GTS) observed in hepatotoxic rats too showed signs of retuming towards normalcy in MEC co-administered animals, thus corroborating the antioxidant efficacy of MEC. The findings provide a rationale for further studies on isolation of active principles and its pharmacological evaluation.  相似文献   

耐力训练及限食对老龄大鼠骨骼肌线粒体氧化及抗氧化水平影响的比较研究     

曾正中  文立  郝俊琴  吴志义  张勇 《体育科技文献通报》2014,(3):41-42,46

目的:观察长期耐力训练及限食对老龄大鼠骨骼肌线粒体氧化及抗氧化水平的影响,比较其单独及协同作用,为"线粒体衰老"学说提供一定的理论依据。方法:32只17月龄雄性SD大鼠分为4组:安静组(Control,C)、限食组(Caloric-Restricted,CR)、运动组(Exercise,E)和限食加运动组(Caloric-restricted and Exercise,E+CR),训练方式为跑台运动,中等运动强度(64%VO2max,15m/min,60分钟/天,每周5天),限食摄入的标准为正常摄入组的60%,共训练及限食12周,相同月龄对照组正常饲养。12周后于末次训练后取大鼠骨骼肌分别进行线粒体氧化损伤水平及抗氧化水平测定。结果:CR组、E组和CR+E组骨骼肌线粒体丙二醛(malondialdehyde,MDA)含量显著降低,锰超氧化物歧化酶(manganese-containing superoxide dismutase,MnSOD)活性显著增加,谷胱甘肽过氧化物酶(glutathion peroxidase,GSH-PX)活性显著增加;E组和CR+E组骨骼肌线粒体过氧化氢酶(catalase,CAT)活性显著增加。结论:耐力训练提高机体的抗氧化水平,同时使细胞机能节省化,耗氧量相对下降而减少了自由基的产生,限食减弱了机体的氧化应激,减少了自由基的产生,阻止了脂质过氧化,同时限食动员了一系列的适应性的网络防御机制,增加了抗氧化水平,加强了防御。耐力运动在机体抗氧化能力上明显强于限食作用,而耐力运动与限食的协同作用对某些抗氧化酶的影响强于其单独作用的影响。  相似文献   

植物水分胁迫与活性氧保护酶   总被引：3，自引：0，他引：3  

闫成仕 《常熟理工学院学报》2002,16(4):56-58

介绍了植物体活性氧酶促保护系统的组成及其对不同水分胁迫条件的响应，并对今后进一步的研究作出了展望。  相似文献   

尿路感染病原菌分布及大肠埃希菌的耐药性分析     

宋静静  张群智 《大理师专学报》2013,(12):39-42

目的：了解保山某院尿路感染病原菌的分布情况及大肠埃希菌的耐药情况,为临床合理使用抗生素提供依据。方法：用VITET-2Compact60全自动微生物分析仪鉴定细菌,K—B纸片扩散法做体外药敏试验,统计、分析细菌的检出率和药敏结果。结果：尿路感染病原菌占第一位的是大肠埃希菌（74．2％）,其次为肺炎克雷伯菌（10％）,次之为表皮葡萄球菌（4.3％）;病原菌在临床各科室分布情况为泌尿科45．7％、康复科20％、ICU14.3％;52株大肠埃希菌对13种抗生素的耐药率居前三位的分别为：氨苄西林98％、复方新诺明76．9％、庆大霉素65．4％,52株大肠埃希菌对厄他培南、亚胺培南均敏感;52株大肠埃希菌中产ESBLsIg／29株占55．8％,非产ESBLs的23株占44．2％。结论：医院尿路感染病原菌以大肠埃希菌为主,科室分布以泌尿科为首,尿路感染大肠埃希菌的耐药情况较严重,医院应加强其耐药性监测,医生应依据体外药敏试验结果合理选择抗生素。  相似文献   

Enzyme activity and myoglobin concentration in rat myocardium and skeletal muscles after passive intermittent simulated altitude exposure     

Santi Esteva  Pere Panisello  Joan Ramon Torrella  Teresa Pagés 《Journal of sports sciences》2013,31(6):633-640

Abstract

We studied the effect of intermittent hypobaric hypoxia exposure on lactate dehydrogenase and citrate synthase activities, together with myoglobin content, of rat myocardium, tibialis anterior, and diaphragm muscles. The intermittent hypoxia exposure programme consisted of daily 4-h sessions in a hypobaric chamber (5000 m) over a period of 22 days. Samples were taken at the end of the programme, and 20 and 40 days later, and compared with those of control animals. In myocardium, lactate dehydrogenase activity was significantly depressed in animals 20 days post-exposure (314.6 ± 15.3 IU · g^?1) compared with control animals (400 ± 14.3 IU · g^?1), while citrate synthase activity and myoglobin concentration showed a significant stepwise increase from control animals (88.2 ± 3.6 IU · g^?1 and 4.38 ± 0.13 μm · mg^?1) to animals 20 days (104.7 ± 3.7 IU · g^?1 and 5.01 ± 0.17 μm · mg^?1) and 40 days post-exposure (108.8 ± 6.5 IU · g^?1 and 5.11 ± 0.22 μm · mg^?1). In contrast, no differences were found in diaphragm and tibialis anterior muscles. Our results show that intermittent hypobaric hypoxia exposure increased the oxidative character of myocardium even 20 days after the hypoxic stimulus has ceased, and that this effect lasts for more than 40 days for citrate synthase activity and myoglobin concentration. These findings support our previous results on skeletal and cardiac muscle capillarization after passive intermittent simulated altitude exposure, thus providing morphofunctional and biochemical evidence for increased cardiac aerobic efficiency.  相似文献   

Physical Activity as a Natural Antioxidant Booster and its Effect on a Healthy Life Span     

Helaine M. Alessio  Eileen R. Blasi 《Research quarterly for exercise and sport》2013,84(4):292-302

Abstract

The recent Surgeon General's report (U.S. Department of Health and Human Services, 1996) promotes regular physical activity to enhance health. A possible mechanism by which physical activity contributes to a healthy life span is via enhanced antioxidant status. The purpose of this paper is to summarize findings from human studies on life span, health, antioxidants, and the effectiveness of physical activity as a natural antioxidant booster. Epidemiological studies concur that some antioxidants are inversely related to mortality. A single bout of exercise elevates some antioxidant enzyme and coenzyme activities. Regular physical activity enhances some antioxidants; however, strenuous exercise may diminish others. Results generally show that antioxidants play a mediating role in the way in which physical activity positively affects a healthy life span.  相似文献   

A biochemical study on variability of Superoxide dismutase,Catalase and Glutathione peroxidase in dry cleaners     

S. Anbhazhagan  Santosh S. Winkins 《Indian journal of clinical biochemistry : IJCB》2008,23(2):198-199

Several chemicals which induce cytotoxicity are broadly classified into three types and they are organophosphate, organochlorine and carbomate compounds. Perchloroethylene has been clearly identified as a carcinogen in experimental animals and it’s a very common carcinogen used for dry clean purpose. Hence, in the present study, blood samples from dry cleaners were taken, Superoxide dismutase, Catalase and Glutathione peroxidase activity was estimated in blood samples of dry cleaners. There was a significant increase in the activity of Catalase, Glutathione Peroxidase, Superoxide Dismutase in dry cleaners of chronically exposed to perchloroethylene as compared to controls. The increased activities of antioxidant enzymes may be a compensatory regulation in response to increased oxidative stress induced by perchloroethylene.  相似文献