桐花树叶多酚提取物的抗氧化活性及其影响因子     

黄晓冬  李裕红  黄晓昆  黄晓玲 《泉州师范学院学报》2014,(2):1-6

采用总抗氧化能力体系、羟自由基清除体系、螯合铁离子能力体系测定评价桐花树叶多酚提取物的抗氧化活性,同时在DPPH·自由基清除体系下进行该多酚提取物自由基清除能力的影响因子分析．结果表明：桐花树叶多酚提取物的总抗氧化能力为38U／mg,清除羟自由基的IC50为0．008mg／mL,螯合铁离子能力的IC50约为42mg／mL,表现出较强的抗氧化性能;在DPPH·自由基清除体系下,考察添加食品添加剂或金属离子对桐花叶多酚提取物清除自由基活性的影响,结果显示：苯甲酸钠、柠檬酸与Mn^2＋、Cu^2＋、Ca^2＋、Fe^3＋等金属离子表现为抑制作用,香兰素与K^＋、Na^＋、Mg^2＋、Al^3＋、Zn^2＋等金属离子表现为增效作用．  相似文献   

巫山神茶水提物的体外抗氧化及抗突变效果     

周露  赵欣  李贵节  付利平 《常熟理工学院学报》2014,(4):75-77

对购买的巫山神茶进行体外抗氧化及抗突变效果评价.通过体外实验分析,研究巫山神茶的功能性效果.研究结果表明,高浓度的巫山神茶水提取物比低浓度茶具有更好的羟基自由基和DPPH自由基清除能力.1000 mg/kg浓度巫山神茶水提取物也表现出最好的抗突变效果.综合分析得出,一定浓度的巫山神茶水提取物具有较好的体外功能性效果.  相似文献   

商洛何首乌提取物的抗氧化活性研究     

贾朝 《商洛师范专科学校学报》2014,(4):15-18

为了研究商洛何首鸟提取物抗氧化能力。采用DPPH、超氧阴离子、亚硝酸基和总还原能力等方法对商洛何首乌提取物和二苯乙烯苷进行体外抗氧化活性研究。结果表明：商洛何首鸟提取物具有抗氧化活性,并且何首乌提取物抗氧化活性高于二苯乙烯苷的抗氧化活性。  相似文献   

枇杷根与枇杷叶的抗氧化活性比较     

曹红云  洪燕萍  邱丰艳 《衡水学院学报》2013,(4)

试验测定枇杷根与枇杷叶中的黄酮、总酚的含量及抗氧化活性的大小。结果表明在枇杷叶、枇杷根皮、枇杷根木质部3个部位中,根皮的黄酮、总酚含量最高,根木质部较低、叶最低。通过DPPH法和FRAP法抗氧化能力的测定,枇杷根皮抗氧化活性最强、根木质部较弱、叶最差。黄酮、总酚含量与自由基清除能力及总还原能力均呈极显著的正相关。  相似文献   

三唑酮对蚕豆离体叶片中三种抗氧化酶活性的影响     

段辉国  张轩波  向利红  谭春华  杨汉波  李清 《内江师范学院学报》2009,24(6):26-28

以蚕豆叶片为试材,对其进行黑暗诱导衰老,研究了三唑酮处理对蚕豆离体叶片过氧化物酶（POD）、超氧化歧化酶（SOD）、过氧化氢酶（CAT）活性的影响．结果显示,在蚕豆离体叶片衰老过程中,POD活性不断升高,特别是在48h之前十分明显,SOD,CAT活性在48h之前上升,然后有所下降;经三唑酮处理蚕豆离体叶片,三种抗氧化酶活性在处理的前期上升速率明显加快,而且维持上升的时间更长．在整过处理过程中三种抗氧化酶活性均远高于对照组．表明三唑酮处理蚕豆离体叶片后,可以通过提高并维持三种抗氧化酶的活性,增强蚕豆叶片抗氧化能力,进而延缓其衰老进程．  相似文献   

A Rethinking on the Benefits and Drawbacks of Common Antioxidants and a Proposal to Look for the Antioxidants in Allium Products as Ideal Agents: A Review     

K. T. Augusti  Regi Jose  G. R. Sajitha  Paul Augustine 《Indian journal of clinical biochemistry : IJCB》2012,27(1):6-20

This review article on the beneficial uses of Allium antioxidants tries to give some answers to the recent doubts raised by Singh et al. (Ind J Clin Biochem 25(3):225–243, 2010) against the claim of some researchers that Antioxidants (AOs) are miraculous molecules. Many people still believe that vitamins like A, C and E are the only true AOs that play important role in the corrections of metabolic derangements in life style diseases and hence all their faults are attributed to the failures of AOs as a class. This is quite unfair as there are many other natural AOs that do equal or even better AO action than the vitamins. Such is the case with the Allium S-alkyl sulfoxide aminoacids and their breakdown products viz, the various poly sulfides and their oxides e.g. allicin and ajoene type compounds which trap electrons mainly. It is true that antioxidant vitamins and β-carotene a precursor of Vitamin A bring about problems as prooxidant or as agents that block some metabolic pathways and gene expression. Again the argument that AOs cannot improve the level of antioxidant enzymes like SOD, catalase and glutathione Px is also not universal. Actually allium AOs can even spare the use of antioxidant vitamins in the body and enhance the action of antioxidant enzymes and supply of ATP and other nutrients to the tissues as the former are good vasodialators and promoters of membrane permeability. The use of AOs should be selective and moderate. Allium AOs satisfy the role of ideal AOs based on many of their invivo and invitro actions reported by the author and others. Their metabolits can regenerate them and recycle them for a sufficient time in the body. They have non antioxidant effects also such as antiplatelet, fibrinolytic, antiinflammatory, immunomodulatory, antiageing actions etc. Plant derived AOs may be more beneficial and better tolerated in their partially purified forms rather than in their absolutely purified forms as the accompanying principles have some protective and regulatory effects in general. This and other aspects of allium AOs are discussed in the paper.  相似文献   

模拟3500m不同低氧训练模式对大鼠力竭运动后心肌线粒体自由基代谢及呼吸链功能的影响     

李洁  邢良美 《上海体育学院学报》2012,(1):51-55

目的:探讨4种低氧训练模式对大鼠力竭运动后心肌线粒体自由基代谢及呼吸链功能的影响。方法:将雄性Wistar大鼠随机均分为5组,即低住低训组(LoLo)、高住高练组(HiHi)、高住低训组(HiLo)、低住高练组(LoHi)及高住高练低训组(HiHiLo)。以当地环境为基点作为常氧环境,采用低压氧舱模拟低氧环境。依实验方案,各组大鼠分别在常氧或/和低氧环境中居住及递增负荷训练5周,每周训练6 d。各组大鼠在最后1次训练后,在常氧环境中恢复3 d,力竭运动后即刻取心肌样本。用差速离心法提取心肌线粒体,分别测定丙二醛含量、超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶(catalase,CAT)活性及呼吸链酶复合体I～IV(CI～IV)活性。结果:与LoLo组相比,心肌组织中HiHi组GSH-Px及CAT活性均显著升高,HiLo组CAT活性显著升高,LoHi和HiHiLo组SOD、GSH-Px及CAT活性均显著升高,4种低氧训练模式MDA含量均显著降低。与LoLo组相比,HiHiLo组心肌线粒体SOD活性显著升高,HiHi、HiLo和HiHiLo组MDA含量均显著降低。与LoLo组相比,HiHi组心肌线粒体CIV活性显著升高,HiHiLo组CI、CII和CIV活性均显著提高,HiHi、LoHi及HiHiLo组CIII活性均显著降低。结论:HiHiLo可能是较好的低氧训练模式。  相似文献   

Protection of salvianolic acid B for human endothelial cells against hydrogen peroxide-induced oxidative damage     

张俊刚  赵广荣  刘金玲  季祥武 《天津大学学报(英文版)》2009,15(6):434-439

Salvianolic acid B(Sal B) is an active component of traditional Chinese medicine Salvia miltiorrhiza and is used to treat vascular diseases. To better understand its mechanism, the antioxidant capacities of Sal B was evaluated with human endothelial cells under oxidative stress. Human endothelial cells were pretreated with Sal B for 12 h followed by hydrogen peroxide for another 12 h. Production of reactive oxygen species (ROS), activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), and concentration of glu-tathione were measured: Protective effect of Sal B on the endothelial cells from hydrogen peroxide-induced damage ' was observed, and ROS production in the cells was found significantly inhibited. Sal B remarkably enhanced the activities of antioxidant enzymes SOD, CAT and GPX. Furthermore, Sal B up-regulated the intracellular glutathione concentration. The results indicate that Sal B protected endothelial cells from oxidative stress by improving the redox status of the cells through enhancing the antioxidant enzyme activities and increasing the reductive glutathione concentration after the oxidative challenge.  相似文献   

Oxidative stress in elite athletes training at moderate altitude and at sea level     

Josefa León-López  Carmen Calderón-Soto  Matías Pérez-Sánchez  Belén Feriche  Xavier Iglesias  Diego Chaverri 《European Journal of Sport Science》2018,18(6):832-841

Using a controlled parallel group longitudinal trial design, we investigated the effects of different training interventions on the prooxidant/antioxidant status of elite athletes: living and training at moderate altitude for 3 (Hi-Hi3) and 4 weeks (Hi-Hi), and for 4 weeks too, living high and training high and low (Hi-HiLo) and living and training at sea level (Lo-Lo). From 61 swimmers, 54 completed the study. Nitrites, carbonyls, and lipid peroxidation (LPO) levels were assessed in plasma. Enzymatic antioxidants glutathione peroxidase (GPx) and glutathione reductase (GRd), and non-enzymatic antioxidants total glutathione (GST), reduced glutathione (GSH) and oxidized glutathione (GSSG) were analysed in the erythrocyte fraction. At the end of the intervention, nitrites levels were similar in all altitude groups but higher than in the Lo-Lo controls (P?=?.02). Hi-HiLo had greater GPx activity than Hi-Hi and Hi-Hi3 during most of the intervention (P?≤?.001). GRd activity was higher in Lo-Lo than in Hi-Hi at the end of the training camp (P?≤?.001). All groups showed increased levels of LPO, except Lo-Lo, and carbonyls at the end of the study (P?≤?.001). Training at altitude for 3 or 4 weeks drives oxidative stress leading to cellular damage mainly by worsening the antioxidant capacities. The GSSG/GSH ratio appears to be related to perceived exertion and fatigue. The stronger antioxidant defence showed by the Hi-HiLo group suggests an inverse relationship between redox alterations and performance. Further studies are required to investigate the role of oxidative stress in acclimatization, performance, and health.  相似文献   

Evaluation of hypoglycemic and antioxidant effect of<Emphasis Type="Italic">Ocimum sanctum</Emphasis>     

Jyoti Sethi  Sushma Sood  Shashi Seth  Anjana Talwar 《Indian journal of clinical biochemistry : IJCB》2004,19(2):152-155

Ocimum sanctum leaves have been traditionally used in treatment of diabetes mellitus. Dietary supplementation of fresh tulsi leaves in a dose of 2 gm/kg BW for 30 days led to significant lowering of blood glucose levels in test group. Intake ofOcimum sanctum also led to significant increase in levels of superoxide dismutase, reduced glutathione and total thiols, but marked reduction in peroxiodised lipid levels as compared to untreated control group. The leaves were found to possess both superoxide and hydroxyl free radical scavenging action. The present observations establish the efficacy ofOcimum sanctum leaves in lowering blood glucose levels and antioxidant property appears to be predominantly responsible for hypoglycemic effect.  相似文献