Yangxueqingnao particles inhibit rat vascular smooth muscle cell proliferation induced by lysophosphatidic acid   总被引：1，自引：0，他引：1  

Cai W  Xu Y  Chen JZ  Huang SR  Lu ZY  Wang ZK 《Journal of Zhejiang University. Science. B》2005,6(9):892-896

Objective: To observe the effect of Yangxueqingnao particles on rat vascular smooth muscle cell (VSMC) proliferation induced by lysophosphatidic acid (LPA). Methods: The amount of3H-TdR (3H-thymidine) admixed in cultured rat VSMC was measured and mitogen-activated protein kinase (MAPK) activity and lipid peroxidation end product malondialdehyde (MDA)content of the VSMC were assayed. Results: 1×10-9, 1×10-8, 1×10-7 mol/L LPA in a concentration dependent manner, induced the amount of 3H-TdR admixed, MAP kinase activity, and MDA content of the cultured rat VSMC to increase. However, 5%, 10%,and 15% Yangxueqingnao serum preincubation resulted in a decrease of 23.0%, 42.0%, and 52.0% (P＜0.01) respectively in the amount of 3H-TdR admixed, a decline in VSMC MAP kinase activity of 13.9% (P＜0.05), 29.6% (P＜0.01), and 48.9% (P＜0.01)respectively, and also, a decrease in MDA content of VSMC of 19.4%, 24.7%, and 43.2% (P＜0.01) respectively, in the 1×10-7mol/L LPA-treated VSMC. Conclusions: LPA activates the proliferation and lipid peroxidation of VSMC in a concentration dependent manner. The LPA-induced VSMC proliferation is related to the activity of MAP kinases, enzymes involved in an intracellular signalling pathway. The results of the present study showed that Yangxueqingnao particles can effectively inhibit LPA-induced VSMC proliferation, MAP kinase activation, and reduce lipid peroxidative lesion.  相似文献   

意大利蜜蜂(Apis mellifera L.)飞翔肌的发育     

叶巧真  卢爱平  张景强 《中山大学学报论丛》1995,(2)

全变态昆虫肌肉的转化与脊椎动物肌肉损伤修复有很大的相似之处。本文以意大利蜜蜂飞翔肌的发育阐明这一观点。两者的相似性表现为:①参与肌肉修复和成蜂飞翔肌形成的成肌细胞来源于胚细胞;②飞翔肌的发育过程中肌肉的转化和肌肉损伤修复均有幼虫残余肌丝或损伤部位残余肌丝和成肌细胞融合,成肌细胞与成肌细胞之间的融合等现象。这些结果为全变态昆虫肌肉的转化作为了解脊椎动物肌肉再生知识的便利模型的可行性,提供了理论依据。  相似文献   

碱胁迫对叶片延迟发光的实验研究   总被引：1，自引：0，他引：1  

王红梅 《实验技术与管理》2011,(10):52-55

选用绿宝石喜林芧叶片为材料,用BPCL型微弱发光测量仪测量了叶片经高浓度NaOH溶液处理后的延迟发光情况。结果发现:同一个叶片随着处理时间的延长,其延迟发光最初值和最后100s的光子数都先有一个小幅度的上升,然后又迅速下降;不同的叶片在相同的处理时间下,随着NaOH质量分数的增加,延迟发光最初值和最后100s的光子数都呈现上升的趋势,NaOH质量分数越大,上升得越快,随着处理时间的延长,上升的趋势变得越来越缓,最后各曲线越来越接近,且趋于平稳。整个实验表明,延迟发光可以规律性地反应碱胁迫对叶片的伤害程度,这为生物物理方法测量外界胁迫对叶片的影响提供了参考。  相似文献   

Rapid phenotypic evolution with shallow genomic differentiation during early stages of high elevation adaptation in Eurasian Tree Sparrows     

Yanhua Qu  Chunhai Chen  Ying Xiong  Huishang She  Yong E Zhang  Yalin Cheng  Shane DuBay  Dongming Li  Per G P Ericson  Yan Hao  Hongyuan Wang  Hongfeng Zhao  Gang Song  Hailin Zhang  Ting Yang  Chi Zhang  Liping Liang  Tianyu Wu  Jinyang Zhao  Qiang Gao  Weiwei Zhai  Fumin Lei 《国家科学评论(英文版)》2020,7(1):113

Known as the ‘third polar region’, the Qinghai-Tibet Plateau represents one of the harshest highland environments in the world and yet a number of organisms thrive there. Previous studies of birds, animals and humans have focused on well-differentiated populations in later stages of phenotypic divergence. The adaptive processes during the initial phase of highland adaptation remain poorly understood. We studied a human commensal, the Eurasian Tree Sparrow, which has followed human agriculture to the Qinghai-Tibet Plateau. Despite strong phenotypic differentiation at multiple levels, in particular in muscle-related phenotypes, highland and lowland populations show shallow genomic divergence and the colonization event occurred within the past few thousand years. In a one-month acclimation experiment investigating phenotypic plasticity, we exposed adult lowland tree sparrows to a hypoxic environment and did not observe muscle changes. Through population genetic analyses, we identified a signature of polygenic adaptation, whereby shifts in allele frequencies are spread across multiple loci, many of which are associated with muscle-related processes. Our results reveal a case of positive selection in which polygenic adaptation appears to drive rapid phenotypic evolution, shedding light on early stages of adaptive evolution to a novel environment.  相似文献   

投掷运动员体成分的调查分析   总被引：1，自引：0，他引：1  

吕新颖 《北京体育大学学报》1999,(3)

通过对受试者体成分皮褶厚度的测定,并对其派生指标的比较发现：投掷选手皮脂较厚,其中重器械投掷选手达到肥胖标准。分析认为,这是由于其肌肉体积逐渐增大的同时,总伴随着皮下脂肪逐渐堆积的结果。肥胖不仅影响运动的效果,对身体健康也不利。建议投掷选手在促使肌肉体积增大的同时应积极采取消除脂肪的措施。  相似文献   

跨栏技术的运动解剖学分析     

潘春梅  金丽颖 《山西师大体育学院学报》1995,(1)

跨栏运动员在提高短跑速度的同时,如何改进跨栏技术?本文针对摆动腿、起跨腿、摆臂三方面的问题,从运动解剖学角度进行分析,提出加强后蹬肌群、肩带肌群和盆带肌群在力量、柔韧性和灵活性等方面的训练,从而提高全身动作的协调性,促进跨栏技术的提高。  相似文献   

停训和减量训练对骨骼肌的影响   总被引：1，自引：0，他引：1  

周思红  周里 《西安体育学院学报》1998,(4)

笔者着重从运动员停训和减量训练而导致对骨骼肌的影响进行综述,从而使体育科研工作者利用停训和减量训练理论,去科学地进行指导训练和比赛。  相似文献   

不同方式的力量练习对血尿某些生化指标的影响     

张蕴琨  蒋晓玲 《体育与科学》1998,19(4):39-41

本文观察了两种不同方式的力量练习对血清CK、Mb和尿3-MH水平及肌肉酸痛的影响。结果显示：第二次练习的负荷递增方式为“阶梯式”,其练习后血清CK、Mb水平和尿3－MH排泄量的升高幅度明显低于第一次“斜坡式”练习后,且恢复较快,肌肉酸痛程度也有所降低。提示：适宜的训练方式可能减轻由于高强度力量训练引起的骨骼肌纤维损伤。  相似文献   

机械牵拉刺激对大鼠骨骼肌卫星细胞增殖的影响     

史仍飞  危小焰  卞玉华 《体育科学》2007,27(5):74-76

目的：探讨机械拉伸刺激对骨骼肌卫星细胞增殖能力的影响。方法：通过Flexercell细胞拉伸力学装置对骨骼肌卫星细胞施加5％、15％和25％拉伸应变加载实验，用CCK-8法检测细胞受机械刺激后的增殖变化，采用BCA蛋白测定试剂盒测定细胞总蛋白含量。结果：骨骼肌卫星细胞受机械张力刺激后，发现卫星细胞增殖能力增强，蛋白含量增加，其中，15％拉伸应变率的促增殖与对照组有显著性差异（P〈0．01）。结论：机械刺激可以促进体外卫星细胞的增殖能力，细胞增殖能力与细胞所受刺激的负荷大小有关。  相似文献   

健脾补糖对过度训练脾虚大鼠骨骼肌GLUT4和P38活性的影响   总被引：2，自引：0，他引：2  

衣雪洁  张庆荣  于莹 《北京体育大学学报》2007,30(9):1212-1215

为了探讨健脾补糖对过度训练大鼠骨骼肌的糖转运和MAPK转导通路中P38活性的影响.通过6周递增大负荷游泳训练,观察过度训练和健脾补糖对胃肠的吸收功能,骨骼肌的糖转运、储备和P38活性的影响.研究表明:1)过度训练脾虚的大鼠的骨骼肌MAPK信号转导系统的P38途径虽然被激活.但骨骼肌的葡萄糖转运和糖储存被低血糖和胰岛素所抑制.因此没有明显改善骨骼肌的肌糖原储备.2)健脾补糖从改善胃肠功能、增加能源物质的摄入、提高抗氧化能力等多靶点激活了P38,使骨骼肌对葡萄糖的转运速度与合成速度均加快,在末次运动后24 h就使肌糖原达到了明显的超量恢复状态.  相似文献