裸子植物的生化系统学(三)——从种子蛋白多肽和针叶过氧化物酶探讨红豆杉科的系统位置     

胡志昂  王洪新  刘长江  潜忠兴 《中国科学院研究生院学报》1986,24(4):260-263

 SDS聚丙烯酰胺凝胶电泳揭示红豆杉属和白豆杉的种子蛋白主要多肽的分子量为31、   22和20千道尔顿(K)。穗花杉的多肽谱与上述两属相差较大，没有22K多肽，代之以一个33K   主要多肽。榧树属和穗花杉有很多共同的多肽，但没有44K这个红豆杉科多数种类共有的中   等含量的多肽，并出现一个36K主要多肽。三尖杉属一些种的多肽谱十分接近红豆杉属，竹   柏的种子蛋白与上述分类群也有一定程度的近似。     红豆杉科各属之间针叶过氧化物酶谱差别很大，但三尖杉属一些种与红豆杉属却有些类   似。     两种蛋白质资料一致说明红豆杉科内的进化趋势是从红豆杉属、经白豆杉属和穗花杉属   至榧树属。红豆杉属和三尖杉属之间蛋白质的近似，说明红豆杉科和三尖杉科之间的关系，可 能通过红豆杉属相联系的,述资料也说明红豆杉科应置于松柏目之下。  相似文献   

分子伴侣、蛋白质聚集和大分子拥挤环境对蛋白质折叠的影响(英)     

李剑  王志珍 《中国科学院研究生院学报》2002,(2)

研究了细胞内存在的分子伴侣、蛋白质聚集和大分子拥挤环境对蛋白质折叠的影响.首先,发现分子伴侣GroEL与底物蛋白的结合有 半位 "和 全位"两种模式,它是由底物蛋白的分子形状、分子大小以及与GroEL的相互作用性质决定的.接着,发现两种不同的蛋白质一起复性时相互不干扰,提示细胞内蛋白质折叠可能不受其他蛋白聚集的影响;后又发现α 乳清蛋白的前熔球态不仅是分子伴侣也是蛋白质聚集体的作用对象.最后,研究大分子拥挤环境对蛋白质折叠热力学和动力学的影响,揭示了这种影响的复杂性和多样性.  相似文献   

乳腺癌耐受蛋白介导5-氟脲嘧啶的耐受及机制探讨     

袁建辉  贺智敏  吕辉  余艳辉  陈主初 《深圳特区科技》2005,(Z1):106-112

AIM To filtrate breast cancer resistance protein(BCRP)-mediated resistance agents and investigate the mechanism,so as to provide valuable datum for optimization clinical chemotherapy scheme to tumor with evaluation marker of BCRP expression. METHODS MTT assay was used to filtrate BCRP-mediated resistance agents with PA317/Tet-on/TRE-BCRP cell of different expression levels of BCRP after treated with different concentration anticancer agents. High performance liquid chromatography(HPLC) was applied to measure relative dose of intracellular retention resistance agents. Nuclear DNA fluorescence dye,Hochest 33258, staining and flow cytometry were adopted to detect apoptotic cells after treated with drugs. RESULTS There were shown increasing durg-resistance to 5-fluorouracil,methotrexate, doxirubicin, pirarubicin,etoposide and mitoxantrone followed with increasing expression of BCRP on PA317/Tet-on/TRE-BCRP cells(P＜0.05, n=3),but shown sensitive to paclitaxel, cisplatin, vincristine, mitomycin and vindesine. There also was shown significant negative correlation between the intracellular retention dose of 5-fluorouracil with different expression of BCRP(r=-0.885, P＜0.05, n=3).There were shown parallel results of that decreasing cellular apoptotic rate with increasing cellular expression of BCRP after treated with 5-fluorouracil by fluorescence dye staining and flow cytometry(P＜0.05, n=3),and also shown significate rise of the apoptotic rate of BCRP expression cells after treated with Ko143 (P＜0.05, n=3). Every group of cells could be different extently blocked in phase of G0/G1 treated with 5-fluorouracil. CONCLUSION Resistance of 5-fluorouracil could be especially mediated by conjugated with BCRP and acted as drug exclude-pump substrate. Cellular ability resistant to 5-fluorouracil-induced apoptosis could be reinforced by BCRP expression.  相似文献   

玉兰减数分裂观察及染色体构型分析     

李建强  何子灿 《中国科学院研究生院学报》2003,41(4):362-368

采用去壁低渗方法，观察研究了玉兰Magnolia denudata有丝分裂和减数分裂的细胞学特征。实验结果证实玉兰存在两种染色体倍性，即2n=4x=76和2n=6x=114。通常，在木兰属甚至整个木兰科每个物种只具有一种染色体数目。玉兰有丝分裂间期核为复杂染色中心型，其中期染色体较小。玉兰在减数分裂中期I的构型表现出多样性，其中最主要的特点是比同源多倍体预期的二价体出现的频率更高些，其次是在减数分裂中期I可以观察到1或2个环状和（或）链状六价体。这些特征与同源异源六倍体或部分的异源六倍体种北美红杉Sequ  相似文献   

羊皮下脚料中明胶和水解蛋白的提取研究   总被引：1，自引：0，他引：1  

赵宏樵  王方国 《科技通报》1995,11(3):171-174

采用化学及生物化学处理方法，从羊皮下脚料中提取明胶和水解蛋白，通过正交多因子试验，确定最佳化学反应条件及分离方法，试验结果证明：明胶的提取率为８％，水解蛋白的提取率为２５％。  相似文献   

Effect of thiazide drug compound on the testicular protein and cholesterol contents of albino rat     

Hemlata Yadav  P. K. Singh 《Indian journal of clinical biochemistry : IJCB》2003,18(2):206-208

In this study the effect of thiazide diuretic compound on the protein and cholesterol contents in the testes of albino rats as the experimental model. The drug thiazide was administered orally daily for 10,20 and 30 days at the dose of 100mg/kg body weight. Total protein decrease in the testes of rats were evidenced may be due to the side effects of thiazide drug compound which is linked with the hyponatremia and protein metabolism. An elevated level of cholesterol contents observed in thiazide treated rats also revealed that the side effect of drug compound thiazide and also may be due to the stimulation of catecholamine which is stimulated therefore, the biochemical estimation such as protein and cholesterol in the testes after the thiazide treatment determined the effectiveness of diuretic drug compound would provide clinical evidences of their side effects.  相似文献   

虫媒登革病毒的研究——Ⅰ.Den 2病毒非结构蛋白NS1从感染细胞膜中的分离     

许琳  陈焕辉  刘乐和  郭辉玉 《中山大学学报论丛》1995,(2)

应用Con-A亲和层析方法从两株Den2病毒(New Guinea C株和从中国海南省分离的H-87株)感染的C6/36细胞膜中分离的糖蛋白,发现了一种感染细胞中特有的蛋白质。经SDS-PAGE证明该蛋白分子量约46000D,分析其为Den 2病毒的非结构蛋白NS1。并利用Western blot筛选出针对该蛋白4种单克隆抗体。  相似文献   

LONG-TERM INHIBITION OF Na~ /H~ EXCHANGE ATTENUATES CARDIAC REMODELING AFTER MYOCARDIAL INFARCTION IN RATS     

XIA Qin-gui  *L  Oliver Chung  Heidi Spitznagel  Thomas Unger 《Journal of Zhejiang University. Science. B》2001,(4)

ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅＮａ /Ｈ ｅｘｃｈａｎｇｅｒ (ＮＨＥ)ｉｓａｐＨ ｒｅｇｕｌａｔｏｒｙｐｒｏｔｅｉｎｐｒｅｓｅｎｔｉｎｔｈｅｐｌａｓｍａｍｅｍ ｂｒａｎｃｅｏｆｃａｒｄｉｏｍｙｏｃｙｔｅｓａｎｄｏｔｈｅｒｃｅｌｌｔｙｐｅｓ.ＡｌｔｈｏｕｇｈｓｅｖｅｒａｌｉｓｏｆｏｒｍｓｏｆＮＨＥｈａｖｅｂｅｅｎｄｅ ｃｒｉｂｅｄ,ｔｈｅｐｒｅｄｏｍｉｎａｎｔｉｓｏｆｏｒｍｉｎｔｈｅｈｅａｒｔｉｓｔｈｅｕｂｉｑｕｉｔｏｕｓＮＨＥ 1 ,ｗｈｉｃｈｕｎｄｅｒｃｅｎｔａｉｎｐｈｙｓｉｏｌｏｇｉｃａｌｃｏｎｄｉｔｉ…  相似文献   

植物中Ca2+调节的蛋白激酶的研究进展   总被引：1，自引：0，他引：1  

萧蓓蕾  冯建英  贾海慧  李冬梅 《宜春学院学报》2006,28(4):113-119

钙(Ca2 )是多种信号途径的第二信使,蛋白质磷酸化与脱磷酸化是信号传递的主要途径.蛋白激酶主要催化蛋白质的磷酸化作用,参与细胞信号识别与转导.Ca2 调节的蛋白激酶与细胞Ca2 信号进一步传递有密切的关系.  相似文献   

Basic fibroblast growth factor alleviates brain injury following global ischemia reperfusion in rabbits   总被引：9，自引：0，他引：9  

Zhang M  Ma YF  Gan JX  Jiang GY  Xu SX  Tao XL  Hong A  Li JK 《Journal of Zhejiang University. Science. B》2005,6(7):637-643

The aim of this study was to explore the protective effect of basic fibroblast growth factor (bFGF) on brain injury following global ischemia reperfusion and its mechanisms. Brain injury following global ischemia was induced by four vessels occlusion and systemic hypotension. Twenty-four rabbits were randomized into three groups: group A, only dissection of vessels; group B, intravenous infusion of normal saline after reperfusion for 6 h; group C, 30 μg/kg bFGF injected intravenously at the onset of reperfusion, then infused with 10 μg/(kg·h) for 6 h. Serum neuron specific enolase (NSE), S-100B, tumor necrosis factor-α(TNF-α), interleukin-1 (IL-1), interleukin-8 (IL-8) were measured before ischemia, 30 min after ischemia, 0.5, 1, 3, 6 h after reperfusion. Brain water content was determined and cerebral histopathological damages were compared. NSE and S-100B were increased 1 h after reperfusion and reached their peaks 6 h after reperfusion, but were much higher in group B than those in group C 3, 6 h after reperfusion. In groups B and C, TNF-a was increased after ischemia and IL-1 and IL-8 were increased significantly 0.5 h after reperfusion, then reached their peaks 6 h, 3 h, 6 h after reperfusion respectively. TNF-a and IL-8 at the time points of 1 h and 3 h and IL-1 at 3 h and 6 h in group C were correspondingly lower than those in group B. These indices in group A were nearly unchanged. There were less severe cerebral histopathological damages in group C compared with group B, but no difference in brain water content. It could be concluded that bFGF alleviates brain injury following global ischemia and reperfusion by down-regulating expression of inflammatory factors and inhibiting their activities.  相似文献