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INTRODUCTION A number of abiotic stresses have been shown to induce programmed cell death (PCD) responses in plant cells. High temperatures (e.g. 55 篊) induce nuclear condensation and DNA fragmentation in cultured carrot, cucumber and Arabidopsis cells (McCabe et al., 1997; McCabe and Leaver, 2000), and also mitochondrial cytochrome c release in cucumber cotyledons (Balk et al., 1999). The PCD pathway also appears to be activated by ozone. Ozone induces an oxidative burst with acc… 相似文献
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Suspension-cultured apple fruit cells (Malus pumila Mill. cv. Braeburn) were exposed to a low oxygen atmosphere to test whether programmed cell death (PCD) has a role in cell dysfunction and death under hypoxic conditions. Protoplasts were prepared at various times after low oxygen conditions were established, and viability tested by triple staining with fluorescein diacetate (FDA), propidium iodide (PI) and Hoechst33342 (HO342). DNA breakdown and phosphatidylserine exposure on the plasma membrane were observed using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and annexin V binding. About 30% of protoplasts from cells after 48 h under low oxygen showed an increased accumulation of HO342, indicating increased membrane permeability. Positive TUNEL and annexin V results were also only obtained with protoplasts from cells under low oxygen. The results suggest that apple cell death under low oxygen is at least partially PCD mediated, and may explain tissue breakdown under controlled atmosphere (low oxygen) conditions in apple fruit. 相似文献
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鸭梨果实采后生理及其气调贮藏气体组分研究 总被引：5，自引：0，他引：5
鸭梨果实采后在２０±１℃时，随着其后熟进程，ＡＣＣ含量和乙烯释放量急剧增加，分别于采后第１５天和第２１天达到峰值，同时伴随出现呼吸跃变．采后果实的果心组织不仅对ＣＯ＿２极为敏感，而且还对低氧敏感．两者导致组织伤害的症状相同，但集中表现的时间不同．ＣＯ＿２伤害多出现在贮藏前期，而低氧伤害则主要在贮藏中、后期出现．鸭梨果实气调贮藏的适宜气体组分为７％～１０％Ｏ＿２和０％Ｃ０＿２． 相似文献
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